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Caspase 14 Antibody (PA5-121009) in WB
Western blot analysis of Caspase 14 in extracts of various cell lines. Samples were incubated with Caspase 14 Polyclonal antibody (Product # PA5-121009) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-121009
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-242 of human Caspase-14 (NP_036246.1).
Conjugate
Form
Liquid
Concentration
1.22 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914581
Product Specific Information
Positive test controls include: HepG2, 293T. The target is usually found in the following locations: Cytoplasm, Nucleus.
Immunogen sequence: MSNPRSLEEE KYDMSGARLA LILCVTKARE GSEEDLDALE HMFRQLRFES TMKRDPTAEQ FQEELEKFQQ AIDSREDPVS CAFVVLMAHG REGFLKGEDG EMVKLENLFE ALNNKNCQAL RAKPKVYIIQ ACRGEQRDPG ETVGGDEIVM VIKDSPQTIP TYTDALHVYS TVEGYIAYRH DQKGSCFIQT LVDVFTKRKG HILELLTEVT RRMAEAELVQ EGKARKTNPE IQSTLRKRLY LQ
Target Information
Caspase 14 encodes a member of the cysteine-aspartic acid protease (caspase) family, which plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. Diseases associated with CASP14 include Ichthyosis, Congenital, Autosomal Recessive 12 and Autosomal Recessive Congenital Ichthyosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apoptosis-related cysteine protease; CASP-14; caspase 14, apoptosis-related cysteine peptidase; caspase 14, apoptosis-related cysteine protease; Caspase-14; Caspase14; MICE; Mini-ICE
Gene Aliases: CASP14; MICE; mini-ICE
UniProt ID: (Human) P31944, (Mouse) O89094
Entrez Gene ID: (Human) 23581, (Mouse) 12365, (Rat) 299587
proteolysis
apoptotic process
epidermis development
keratinization
cornification
activation of cysteine-type endopeptidase activity involved in apoptotic process
intrinsic apoptotic signaling pathway in response to DNA damage
cell differentiation
extrinsic apoptotic signaling pathway in absence of ligand
biological_process
Performance Guarantee
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