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Caspase 14 Antibody (MA5-29082) in ICC/IF
Immunofluorescence staining of Human Caspase 14 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Caspase 14 Monoclonal Antibody (5) (Product # MA5-29082, 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®594-conjugat... View More
Product Details
MA5-29082
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
05
Immunogen
Recombinant Human Caspase-14/CASP14 protein (Ser2-Gln242)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785010
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human Caspase-14/CASP14.
Target Information
Caspase 14 encodes a member of the cysteine-aspartic acid protease (caspase) family, which plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. Diseases associated with CASP14 include Ichthyosis, Congenital, Autosomal Recessive 12 and Autosomal Recessive Congenital Ichthyosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apoptosis-related cysteine protease; CASP-14; caspase 14, apoptosis-related cysteine peptidase; caspase 14, apoptosis-related cysteine protease; Caspase-14; Caspase14
Gene Aliases: CASP14
UniProt ID: (Human) P31944
Entrez Gene ID: (Human) 23581
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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