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Caspase-2 Antibody (600-401-412) in WB
Affinity Purified antibody to Caspase-2 was used at a 1:750 dilution to detect rat caspase-2 in transfected human 292 cell lysates by Western blot. Asterisk indicates a 48 kDa caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. No reaction is observed against human caspase-2. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122) preceded color development using Amersh... View More
Product Details
600-401-412
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.
Conjugate
Form
Liquid
Concentration
0.44 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against rat and mouse caspase-2. The antibody detects long and short forms of the protein. This antibody reacts with mouse and rat caspase-2, but not caspase-2 from human sources. Cross reactivity with caspase-2 from other sources has not been determined. Rat and mouse caspase-2 show 94% homology. There is no significant homology to the human form.
Target Information
Caspase 2 gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CASP-2; Caspase-2; ICH-1 protease; ICH-1L; NEDD-2; Neural precursor cell expressed developmentally down-regulated protein 2; Protease ICH-1
Gene Aliases: CASP-2; Casp2; ICH-1; Ich1; NEDD-2; Nedd2
UniProt ID: (Mouse) P29594, (Rat) P55215
Entrez Gene ID: (Mouse) 12366, (Rat) 64314
luteolysis
neural retina development
proteolysis
apoptotic process
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
brain development
aging
intrinsic apoptotic signaling pathway in response to DNA damage
programmed cell death
protein processing
ectopic germ cell programmed cell death
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
cellular response to mechanical stimulus
apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of apoptotic signaling pathway
Performance Guarantee
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