Invitrogen

Caspase 3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

11 References

View all (56) Caspase 3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Caspase 3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 21

Caspase 3 Antibody (PA5-77887) in ICC/IF

Immunofluorescence analysis of Caspase-3 was performed using 70% confluent log phase NTERA-2 cl.D1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Caspase 3 Polyclonal Antibody (Product # PA5-77887) at 1:200 dilution in 0.1% BSA, incubated at 4 de... View More

Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Caspase 3 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-77887

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2735574

Product Specific Information

Positive Control: HeLa, HeLa (30 µM Cisplatin treatment for 24 hr), PC-12 (30uM cisplatin treatment for 24h), PC-12, Rat2, C8D30, NIH-3T3, Raw264.7, C2C12, Jurkat, Raji, NCI-H929, K562, THP-1, HL-60

Predicted Reactivity: Dog (89%), Cat (87%), Pig (88%), Rabbit (86%), Chimpanzee (99%), Bovine (84%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Oleuropein confers neuroprotection against rotenone-induced model of Parkinson's disease via BDNF/CREB/Akt pathway.

Scientific reports

Singh R,Zahra W,Singh SS,Birla H,Rathore AS,Keshri PK,Dilnashin H,Singh S,Singh SP

Published figure using Caspase 3 polyclonal antibody (Product # PA5-77887) in Western Blot

Sat Feb 11 00:00:00 EST 2023

Protective effect of astaxanthin and metformin in the liver of rats in which the polycystic ovary syndrome model was formed by giving letrozole.

Iranian journal of basic medical sciences

Taştan Bal T,Akaras N,Demir Ö,Ugan RA

PA5-77887 was used in Immunohistochemistry-immunofluorescence to evaluate the effects of astaxanthin on liver tissue in rats with polycystic ovary syndrome.

Wed Jun 07 00:00:00 EDT 2023

Passively-targeted mitochondrial tungsten-based nanodots for efficient acute kidney injury treatment.

Bioactive materials

Huang Q,Yang Y,Zhao T,Chen Q,Liu M,Ji S,Zhu Y,Yang Y,Zhang J,Zhao H,Nan Y,Ai K

PA5-77887 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to provide a new way to passively target mitochondria for AKI, and give inspiration for the treatment of many major diseases closely related to mitochondria, such as myocardial infarction and cerebral infarction.

Wed Mar 01 00:00:00 EST 2023

Silver nanoparticles induced hepatoxicity via the apoptotic/antiapoptotic pathway with activation of TGFβ-1 and α-SMA triggered liver fibrosis in Sprague Dawley rats.

Environmental science and pollution research international

Assar DH,Mokhbatly AA,Ghazy EW,Elbialy ZI,Gaber AA,Hassan AA,Nabil A,Asa SA

PA5-77887 was used in Immunohistochemistry to conclude that the hepatotoxic impact of AgNPs may be regulated by two mechanisms, implying the apoptotic/antiapoptotic pathway via raising BAX and inhibiting Bcl-2 expression levels in a dose-dependent manner.

Tue Nov 01 00:00:00 EDT 2022

Silver nanoparticles induced hepatoxicity via the apoptotic/antiapoptotic pathway with activation of TGFβ-1 and α-SMA triggered liver fibrosis in Sprague Dawley rats.

Environmental science and pollution research international

Assar DH,Mokhbatly AA,Ghazy EW,Elbialy ZI,Gaber AA,Hassan AA,Nabil A,Asa SA

Tue Nov 01 00:00:00 EDT 2022

Cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathy.

BMC neuroscience

Liu P,Li X,Liu J,Zhang H,You Z,Zhang J

PA5-77887 was used in Immunohistochemistry to show that inhibition of α2δ2 could enhance axonal recovery in anterior horn of damaged spinal cord induced by CSM after surgical decompression, providing a potential method for promoting axon regeneration following surgery.

Fri Jul 01 00:00:00 EDT 2022

Protective Effects of Taif Rosewater Against Testicular Impairment Induced By Lead Intoxication In Rats.

Andrologia

Matuq Al-Yasi H,El-Shazly SA,Ahmed EF,Hasan Alamer K,Hessini KY,Attia HA,Alkafafy ME,Mohamed AA,Hassan FA

Published figure using Caspase 3 polyclonal antibody (Product # PA5-77887) in Immunohistochemistry

Thu Jul 01 00:00:00 EDT 2021

Retinal Protection from LED-Backlit Screen Lights by Short Wavelength Absorption Filters.

Cells

Sanchez-Ramos C,Bonnin-Arias C,Blázquez-Sánchez V,Aguirre-Vilacoro V,Cobo T,García-Suarez O,Perez-Carrasco MJ,Alvarez-Peregrina C,Vega JA

PA5-77887 was used in Immunohistochemistry to assess the effects of light emitted by LED tablet screens on pigmented rat retinas with and without optical filters.

Fri Nov 19 00:00:00 EST 2021

Neuroprotective Potential of Bone Marrow-Derived Mesenchymal Stem Cells Following Chemotherapy.

Biomedicines

Sherif IO,Al-Shaalan NH,Sabry D

PA5-77887 was used in Immunohistochemistry to investigate the use of bone marrow-derived mesenchymal stem cells (BM-MSCs) for its therapeutic potential against cisplatin-induced chemobrain as well as various models of brain damage.

Tue Jun 29 00:00:00 EDT 2021

Neuroprotective Potential of Bone Marrow-Derived Mesenchymal Stem Cells Following Chemotherapy.

Biomedicines

Sherif IO,Al-Shaalan NH,Sabry D

Tue Jun 29 00:00:00 EDT 2021

Phytoestrogen genistein hinders ovarian oxidative damage and apoptotic cell death-induced by ionizing radiation: co-operative role of ER-β, TGF-β, and FOXL-2.

Scientific reports

Haddad YH,Said RS,Kamel R,Morsy EME,El-Demerdash E

PA5-77887 was used in Immunohistochemistry to investigate the molecular influence of genistein (GEN) on the ovarian reserve of rats exposed to ϒ-radiation.

Tue Aug 11 00:00:00 EDT 2020

Phytoestrogen genistein hinders ovarian oxidative damage and apoptotic cell death-induced by ionizing radiation: co-operative role of ER-β, TGF-β, and FOXL-2.

Scientific reports

Haddad YH,Said RS,Kamel R,Morsy EME,El-Demerdash E

PA5-77887 was used in Immunohistochemistry to investigate the molecular influence of genistein (GEN) on the ovarian reserve of rats exposed to ϒ-radiation.

Tue Aug 11 00:00:00 EDT 2020

Role of opioid signaling in kidney damage during the development of salt-induced hypertension.

Life science alliance

Golosova D,Palygin O,Bohovyk R,Klemens CA,Levchenko V,Spires DR,Isaeva E,El-Meanawy A,Staruschenko A

PA5-77887 was used in Western Blotting to study the role of opioid receptors (ORs) in intracellular calcium ([Ca2+]i) homeostasis in podocytes and its association with the development of renal damage.

Tue Dec 01 00:00:00 EST 2020

Role of opioid signaling in kidney damage during the development of salt-induced hypertension.

Life science alliance

Golosova D,Palygin O,Bohovyk R,Klemens CA,Levchenko V,Spires DR,Isaeva E,El-Meanawy A,Staruschenko A

Tue Dec 01 00:00:00 EST 2020

Bioinformatics

Protein Aliases: Apopain; Apopain precursor; CASP-3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase3; CPP-32; CPP32beta; Cysteine protease CPP32; ICE3; IRP; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; yama protein

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Gene Aliases: A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama

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UniProt ID: (Human) P42574, (Mouse) P70677, (Rat) P55213

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Entrez Gene ID: (Human) 836, (Mouse) 12367, (Rat) 25402

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Function(s)

protease binding aspartic-type endopeptidase activity cysteine-type endopeptidase activity cyclin-dependent protein serine/threonine kinase inhibitor activity death receptor binding protein binding peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process phospholipase A2 activator activity protein complex binding cysteine-type endopeptidase activity involved in apoptotic process cysteine-type endopeptidase activity involved in execution phase of apoptosis cysteine-type peptidase activity hydrolase activity protease

Process(es)

response to hypoxia B cell homeostasis apoptotic DNA fragmentation proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cellular component disassembly involved in execution phase of apoptosis cellular response to DNA damage stimulus heart development sensory perception of sound learning or memory activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c response to UV response to glucose response to X-ray regulation of macroautophagy protein processing hippocampus development neuron differentiation keratinocyte differentiation erythrocyte differentiation platelet formation negative regulation of B cell proliferation response to cobalt ion response to estradiol response to lipopolysaccharide glial cell apoptotic process response to tumor necrosis factor response to nicotine hippo signaling wound healing response to drug response to hydrogen peroxide T cell homeostasis negative regulation of apoptotic process response to amino acid positive regulation of neuron apoptotic process cell fate commitment negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of activated T cell proliferation response to antibiotic neurotrophin TRK receptor signaling pathway response to glucocorticoid neuron apoptotic process cellular response to organic cyclic compound apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand execution phase of apoptosis response to wounding intracellular signal transduction positive regulation of apoptotic process positive regulation of catalytic activity negative regulation of cell cycle cellular response to organic substance release of cytochrome c from mitochondria extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to oxidative stress response to organic substance response to metal ion response to organic cyclic compound regulation of apoptotic DNA fragmentation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Caspase 3 Polyclonal Antibody

Caspase 3 Antibody (PA5-77887) in ICC/IF

Product Details

PA5-77887

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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