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Invitrogen
Caspase 3 Recombinant Superclonal Antibody (22HCLC)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
Caspase 3 Antibody (710431) in ICC/IF
Immunofluorescent analysis of Caspase-3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Caspase-3 Recombinant Rabbit Polyclonal Antibody (Product # 710431) at a dilution of 1:500 in 1% BSA and incubated for 3 h... View More
Product Details
710431
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
22HCLC
Immunogen
Peptide corresponding to amino acids 30-43 of human Casp3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532716
Product Specific Information
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apopain; Apopain precursor; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase3; CPP-32; Cysteine protease CPP32; ICE3; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1
Gene Aliases: CASP3; CPP32; CPP32B; SCA-1
UniProt ID: (Human) P42574
Entrez Gene ID: (Human) 836
protease binding
aspartic-type endopeptidase activity
cysteine-type endopeptidase activity
cyclin-dependent protein serine/threonine kinase inhibitor activity
death receptor binding
protein binding
peptidase activity
cysteine-type endopeptidase activator activity involved in apoptotic process
phospholipase A2 activator activity
protein complex binding
cysteine-type endopeptidase activity involved in apoptotic process
cysteine-type endopeptidase activity involved in execution phase of apoptosis
protease
response to hypoxia
B cell homeostasis
apoptotic DNA fragmentation
proteolysis
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
cellular component disassembly involved in execution phase of apoptosis
cellular response to DNA damage stimulus
heart development
sensory perception of sound
learning or memory
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
response to UV
response to glucose
response to X-ray
regulation of macroautophagy
protein processing
hippocampus development
neuron differentiation
keratinocyte differentiation
erythrocyte differentiation
platelet formation
negative regulation of B cell proliferation
response to cobalt ion
response to estradiol
response to lipopolysaccharide
glial cell apoptotic process
response to tumor necrosis factor
response to nicotine
hippo signaling
wound healing
response to drug
response to hydrogen peroxide
T cell homeostasis
negative regulation of apoptotic process
response to amino acid
positive regulation of neuron apoptotic process
cell fate commitment
negative regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of activated T cell proliferation
response to antibiotic
neurotrophin TRK receptor signaling pathway
response to glucocorticoid
neuron apoptotic process
cellular response to organic cyclic compound
apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
execution phase of apoptosis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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