Invitrogen

Caspase 3 Monoclonal Antibody (3CSP01 (7.1.44))

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

15 References

View all (56) Caspase 3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

Caspase 3 Antibody (MA5-11516) in IHC (P)

Formalin-fixed, paraffin-embedded human tonsil stained with Caspase 3 antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and nuclear staining of lymphoid cells.

Caspase 3 Antibody in Immunoprecipitation (IP)

Caspase 3 Antibody in Immunohistochemistry (IHC)

Caspase 3 Antibody in Immunocytochemistry (ICC/IF)

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Product Details

MA5-11516

Applications

Tested Dilution

Publications

Western Blot (WB)

1:25-1:50

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

1:25-1:50

Product Specifications

Species Reactivity

Human

Published species

Human, Lizard, Rabbit, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3CSP01 (7.1.44)

Immunogen

Recombinant full length human Caspase 3 protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10983525

Product Specific Information

MA5-11516 targets Caspase 3 in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Human samples.

The MA5-11516 immunogen is recombinant full length human Caspase 3 protein.

Target Information

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Ameliorative Role of Eugenol against Silver Nanoparticles-Induced Hepatotoxicity in Male Wistar Rats.

Oxidative medicine and cellular longevity

Yousef HN,Ibraheim SS,Ramadan RA,Aboelwafa HR

Published figure using Caspase 3 monoclonal antibody (Product # MA5-11516) in Immunohistochemistry

Thu Sep 22 00:00:00 EDT 2022

Human Islet Microtissues as an In Vitro and an In Vivo Model System for Diabetes.

International journal of molecular sciences

Mir-Coll J,Moede T,Paschen M,Neelakandhan A,Valladolid-Acebes I,Leibiger B,Biernath A,Ämmälä C,Leibiger IB,Yesildag B,Berggren PO

Published figure using Caspase 3 monoclonal antibody (Product # MA5-11516) in Immunocytochemistry

Thu Feb 11 00:00:00 EST 2021

Human Islet Microtissues as an In Vitro and an In Vivo Model System for Diabetes.

International journal of molecular sciences

Mir-Coll J,Moede T,Paschen M,Neelakandhan A,Valladolid-Acebes I,Leibiger B,Biernath A,Ämmälä C,Leibiger IB,Yesildag B,Berggren PO

Published figure using Caspase 3 monoclonal antibody (Product # MA5-11516) in Immunocytochemistry

Thu Feb 11 00:00:00 EST 2021

Therapeutic Potentials of Selected Antihypertensive Agents and Their Fixed-Dose Combinations Against Trastuzumab-Mediated Cardiotoxicity.

Frontiers in pharmacology

Olorundare OE,Adeneye AA,Akinsola AO,Ajayi AM,Agede OA,Soyemi SS,Mgbehoma AI,Okoye II,Albrecht RM,Ntambi JM,Crooks PA

Published figure using Caspase 3 monoclonal antibody (Product # MA5-11516) in Immunohistochemistry

Wed Apr 28 00:00:00 EDT 2021

The protective effects of carnosine in alcohol-induced hepatic injury in rats.

Toxicology and industrial health

Baykara B,Micili SC,Tugyan K,Tekmen I,Bagriyanik H,Sonmez U,Sonmez A,Oktay G,Yener N,Ozbal S

MA5-11516 was used in immunohistochemistry to study the mechanisms underlying the protective effects of carnosine in a rat model of alcohol-induced liver damage

Sat Feb 01 00:00:00 EST 2014

Treatment with thyroxine restores myelination and clinical recovery after intraventricular hemorrhage.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Vose LR,Vinukonda G,Jo S,Miry O,Diamond D,Korumilli R,Arshad A,Zia MT,Hu F,Kayton RJ,La Gamma EF,Bansal R,Bianco AC,Ballabh P

MA5-11516 was used in immunohistochemistry and western blot to study the mechanisms by which thyroxine promotes myelination and neurological recovery in a rabbit model of intraventricular hemhorrage

Wed Oct 30 00:00:00 EDT 2013

Survival, integration, and differentiation of unrestricted somatic stem cells in the heart.

Cell transplantation

Ding Z,Burghoff S,Buchheiser A,Kögler G,Schrader J

MA5-11516 was used in immunohistochemistry to study the ability of unrestricted somatic stem cells to survive and differentiate following trans-coronary injection in a rat model

Tue Apr 08 00:00:00 EDT 2014

Erythropoietin prevents lymphoid apoptosis but has no effect on survival in experimental sepsis.

Pediatric research

Köroğlu TF,Yılmaz O,Gökmen N,Tuğyan K,Baskın H,Eğrilmez MY

MA5-11516 was used in immunohistochemistry to study the lack of effect of EPO on survival in a rat model of sepsis despite its ability to inhibit lymphoid apoptosis

Thu Aug 01 00:00:00 EDT 2013

Captopril and telmisartan treatments attenuate cadmium-induced testicular toxicity in rats.

Fundamental & clinical pharmacology

Fouad AA,Jresat I

MA5-11516 was used in immunohistochemistry to study the mechanism by which captopril and telmisartan protect against testicular toxicity induced by cadmium exposure

Mon Apr 01 00:00:00 EDT 2013

Bioengineering functional copolymers. XXI. Synthesis of a novel end carboxyl-trithiocarbonate functionalized poly(maleic anhydride) and its interaction with cancer cells.

Bioorganic & medicinal chemistry

Rzayev ZM,Tűrk M,Söylemez EA

MA5-11516 was used in immunocytochemistry to study the synthesis of a novel bioengineering copolymer with anti-tumor activity and its interaction with cancer cells

Wed Aug 15 00:00:00 EDT 2012

Clinicopathological significance of cathepsin D expression in non-small cell lung cancer is conditional on apoptosis-associated protein phenotype: an immunohistochemistry study.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Fan C,Lin X,Wang E

MA5-11516 was used in immunohistochemistry to study the influence of the phenotype of apoptosis-associated proteins on the clinical significance of cathepsin D expression in non-small cell lung cancer

Wed Aug 01 00:00:00 EDT 2012

Baicalin induces apoptosis in leukemia HL-60/ADR cells via possible down-regulation of the PI3K/Akt signaling pathway.

Asian Pacific journal of cancer prevention : APJCP

Zheng J,Hu JD,Chen YY,Chen BY,Huang Y,Zheng ZH,Liu TB

MA5-11516 was used in western blot to study the role of the PI3 kinase-Akt signaling pathway in the mechanism by which baicalin induces apoptosis in drug-resistant human myeloid leukemia cells

Wed Jan 30 00:00:00 EST 2013

Notch signaling change in pulmonary vascular remodeling in rats with pulmonary hypertension and its implication for therapeutic intervention.

PloS one

Qiao L,Xie L,Shi K,Zhou T,Hua Y,Liu H

MA5-11516 was used in immunohistochemistry to study the role of Notch signaling in pulmonary vascular remodeling in a rat hypoxia-induced model of pulmonary hypertension

Mon Jun 03 00:00:00 EDT 2013

Bioengineering functional copolymers. XIV. Synthesis and interaction of poly(N-isopropylacrylamide-co-3,4-dihydro-2H-pyran-alt-maleic anhydride)s with SCLC cancer cells.

Bioorganic & medicinal chemistry

Türk M,Rzayev ZM,Khalilova SA

MA5-11516 was used in immunocytochemistry to synthesize novel supramolecularlly structured copolymers with anti-cancer activities and study their effects on human lung small cell carcinoma cells

Mon Nov 15 00:00:00 EST 2010

Cadmium induces apoptosis in the pituitary gland of Podarcis sicula.

Annals of the New York Academy of Sciences

Ferrandino I,Favorito R,Annunziata M,Grimaldi MC

MA5-11516 was used in immunohistochemistry to investigate the toxic effect of Cadmium on the pituitary gland of Podarcis sicula

Wed Apr 01 00:00:00 EDT 2009

Increased apoptosis in human osteoarthritic cartilage corresponds to reduced cell density and expression of caspase-3.

Arthritis and rheumatism

Sharif M,Whitehouse A,Sharman P,Perry M,Adams M

MA5-11516 was used in immunohistochemistry to investigate cell death in osteoarthritic cartilage and the role of caspase-3 during this process

Sun Feb 01 00:00:00 EST 2004

Bioinformatics

Protein Aliases: Apopain; Apopain precursor; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase3; CPP-32; Cysteine protease CPP32; ICE3; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1

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Gene Aliases: CASP3; CPP32; CPP32B; SCA-1

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UniProt ID: (Human) P42574

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Entrez Gene ID: (Human) 836

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Function(s)

protease binding aspartic-type endopeptidase activity cysteine-type endopeptidase activity cyclin-dependent protein serine/threonine kinase inhibitor activity death receptor binding protein binding peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process phospholipase A2 activator activity protein complex binding cysteine-type endopeptidase activity involved in apoptotic process cysteine-type endopeptidase activity involved in execution phase of apoptosis protease

Process(es)

response to hypoxia B cell homeostasis apoptotic DNA fragmentation proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cellular component disassembly involved in execution phase of apoptosis cellular response to DNA damage stimulus heart development sensory perception of sound learning or memory activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c response to UV response to glucose response to X-ray regulation of macroautophagy protein processing hippocampus development neuron differentiation keratinocyte differentiation erythrocyte differentiation platelet formation negative regulation of B cell proliferation response to cobalt ion response to estradiol response to lipopolysaccharide glial cell apoptotic process response to tumor necrosis factor response to nicotine hippo signaling wound healing response to drug response to hydrogen peroxide T cell homeostasis negative regulation of apoptotic process response to amino acid positive regulation of neuron apoptotic process cell fate commitment negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of activated T cell proliferation response to antibiotic neurotrophin TRK receptor signaling pathway response to glucocorticoid neuron apoptotic process cellular response to organic cyclic compound apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand execution phase of apoptosis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Caspase 3 Monoclonal Antibody (3CSP01 (7.1.44))

Caspase 3 Antibody (MA5-11516) in IHC (P)

Product Details

MA5-11516

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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