Invitrogen

Caspase 3 Monoclonal Antibody (74T2)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

13 References

View all (56) Caspase 3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 18

Caspase 3 Antibody (43-7800) in IHC (P)

Immunohistochemistry analysis of Caspase-3 showing staining in the cytoplasm of paraffin-embedded human lung adenocarcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at roo... View More

Caspase 3 Antibody in Immunoprecipitation (IP)

Caspase 3 Antibody in Immunocytochemistry (ICC/IF)

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Product Details

43-7800

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Horse, Human, Mouse, Non-human primate, Rat

Published species

Horse, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

74T2

Immunogen

Recombinant protein derived from the N-terminus of human Caspase 3 protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533540

Target Information

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mucosal Recovery after Intestinal Transplantation in the Rat: A Sequential Histological and Molecular Assessment.

European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes

Bagge J,Padma AM,Casselbrant A,Hellström M,Oltean M

43-7800 was used in Immunohistochemistry to suggest that a near-complete morphologic recovery from a moderate mucosal injury occurs within the first 24 hours after intestinal transplantation.

Mon Jun 26 00:00:00 EDT 2023

2-Methoxyestradiol combined with ascorbic acid facilitates the apoptosis of chronic myeloid leukemia cells via the microRNA-223/Fms-like tyrosine kinase 3/phosphatidylinositol-3 kinase/protein kinase B axis.

Bioengineered

Zhang S,Yu H,Li J,Fan J,Chen J

Published figure using Caspase 3 monoclonal antibody (Product # 43-7800) in Western Blot

Tue Feb 01 00:00:00 EST 2022

Metabolic Profiling, Chemical Composition, Antioxidant Capacity, and In Vivo Hepato- and Nephroprotective Effects of Sonchus cornutus in Mice Exposed to Cisplatin.

Antioxidants (Basel, Switzerland)

Elhady SS,Abdelhameed RFA,Mehanna ET,Wahba AS,Elfaky MA,Koshak AE,Noor AO,Bogari HA,Malatani RT,Goda MS

43-7800 was used in Immunohistochemistry to investigate the potential protective effects of Sonchus cornutus extract against cisplatin-induced hepatic and renal toxicity in experimental mice.

Fri Apr 22 00:00:00 EDT 2022

Compatibility of ingredients of Danshen (Radix Salviae Miltiorrhizae) and Honghua (Flos Carthami) and their protective effects on cerebral ischemia-reperfusion injury in rats.

Experimental and therapeutic medicine

Wan H,Yang Y,Li Z,Cheng L,Ding Z,Wan H,Yang J,Zhou H

43-7800 was used in Immunohistochemistry to study the protective effects of the effective ingredients in the Chinese medicines, Danshen and Honghua in cerebrovascular disease.

Sun Aug 01 00:00:00 EDT 2021

The Ganglioside Monosialotetrahexosylganglioside Protects Auditory Hair Cells Against Neomycin-Induced Cytotoxicity Through Mitochondrial Antioxidation: An in vitro Study.

Frontiers in cellular neuroscience

Li Y,Li A,Wang C,Jin X,Zhang Y,Lu L,Wang SL,Gao X

43-7800 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest that GM1 could reduce ROS aggregation, maintain mitochondrial function, and improve HC viability in the presence of neomycin, possibly through mitochondrial antioxidation.

Fri Oct 15 00:00:00 EDT 2021

The Ganglioside Monosialotetrahexosylganglioside Protects Auditory Hair Cells Against Neomycin-Induced Cytotoxicity Through Mitochondrial Antioxidation: An in vitro Study.

Frontiers in cellular neuroscience

Li Y,Li A,Wang C,Jin X,Zhang Y,Lu L,Wang SL,Gao X

Fri Oct 15 00:00:00 EDT 2021

Antibody-drug nanoparticle induces synergistic treatment efficacies in HER2 positive breast cancer cells.

Scientific reports

Abedin MR,Powers K,Aiardo R,Barua D,Barua S

43-7800 was used in Western Blot to engineer antibody-drug nanoparticles by synthesizing pure anti-cancer drug nanorods in the core of nanoparticles with a therapeutic monoclonal antibody.

Thu Apr 01 00:00:00 EDT 2021

Antibody-drug nanoparticle induces synergistic treatment efficacies in HER2 positive breast cancer cells.

Scientific reports

Abedin MR,Powers K,Aiardo R,Barua D,Barua S

43-7800 was used in Western Blot to engineer antibody-drug nanoparticles by synthesizing pure anti-cancer drug nanorods in the core of nanoparticles with a therapeutic monoclonal antibody.

Thu Apr 01 00:00:00 EDT 2021

Iron oxides nanoparticles (IOs) exposed to magnetic field promote expression of osteogenic markers in osteoblasts through integrin alpha-3 (INTa-3) activation, inhibits osteoclasts activity and exerts anti-inflammatory action.

Journal of nanobiotechnology

Marycz K,Sobierajska P,Roecken M,Kornicka-Garbowska K,Kępska M,Idczak R,Nedelec JM,Wiglusz RJ

Published figure using Caspase 3 monoclonal antibody (Product # 43-7800) in Western Blot

Tue Feb 18 00:00:00 EST 2020

Fe₃O₄ Magnetic Nanoparticles Under Static Magnetic Field Improve Osteogenesis via RUNX-2 and Inhibit Osteoclastogenesis by the Induction of Apoptosis.

International journal of nanomedicine

Marycz K,Sobierajska P,Wiglusz RJ,Idczak R,Nedelec JM,Fal A,Kornicka-Garbowska K

43-7800 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to reveal cytocompatibility and anti-inflammatory effects of fabricated nanoparticles.

Tue Oct 11 00:00:00 EDT 2022

5-Azacytydine and resveratrol reverse senescence and ageing of adipose stem cells via modulation of mitochondrial dynamics and autophagy.

Journal of cellular and molecular medicine

Kornicka K,Szłapka-Kosarzewska J,Śmieszek A,Marycz K

43-7800 was used in Western Blotting to evaluate whether treatment of ASCsEMS with a combination of 5-azacytydine and resveratrol would reverse aged phenotype of these cells.

Tue Jan 01 00:00:00 EST 2019

Diazoxide inhibits of ER stress‑mediated apoptosis during oxygen‑glucose deprivation in vitro and cerebral ischemia‑reperfusion in vivo.

Molecular medicine reports

Lei X,Lei L,Zhang Z,Cheng Y

Published figure using Caspase 3 monoclonal antibody (Product # 43-7800) in Western Blot

Fri Jun 01 00:00:00 EDT 2018

Excessive Endoplasmic Reticulum Stress Correlates with Impaired Mitochondrial Dynamics, Mitophagy and Apoptosis, in Liver and Adipose Tissue, but Not in Muscles in EMS Horses.

International journal of molecular sciences

Marycz K,Kornicka K,Szlapka-Kosarzewska J,Weiss C

Published figure using Caspase 3 monoclonal antibody (Product # 43-7800) in Western Blot

Sat Jan 06 00:00:00 EST 2018

Ultrastructural changes during osteogenic differentiation in mesenchymal stromal cells cultured in alginate hydrogel.

Cell & bioscience

Grzesiak J,Śmieszek A,Marycz K

437800 was used in immunocytochemistry to analyze changes occurring during the osteogenic differentiation of mesenchymal stem cells cultured in alginate hydrogel

Thu Oct 01 00:00:00 EDT 2020

Tunneling nanotubes: an alternate route for propagation of the bystander effect following oncolytic viral infection.

Molecular therapy oncolytics

Ady J,Thayanithy V,Mojica K,Wong P,Carson J,Rao P,Fong Y,Lou E

43-7800 was used in Immunohistochemistry to provide proof-of-principle demonstration of a previously unknown and alternative mechanism for inducing apoptosis in noninfected recipient cells.

Tue Sep 29 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Apopain; Apopain precursor; CASP-3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase-3-like protein; Caspase3; CPP-32; CPP32beta; Cysteine protease CPP32; cysteine protease; CPP32; apopain; ICE3; IRP; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; yama protein

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Gene Aliases: A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama

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UniProt ID: (Human) P42574, (Mouse) P70677, (Rat) P55213

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Entrez Gene ID: (Human) 836, (Horse) 100034083, (Mouse) 12367, (Rat) 25402

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Function(s)

protease binding aspartic-type endopeptidase activity cysteine-type endopeptidase activity cyclin-dependent protein serine/threonine kinase inhibitor activity death receptor binding protein binding peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process phospholipase A2 activator activity protein complex binding cysteine-type endopeptidase activity involved in apoptotic process cysteine-type endopeptidase activity involved in execution phase of apoptosis cysteine-type peptidase activity hydrolase activity protease

Process(es)

response to hypoxia B cell homeostasis apoptotic DNA fragmentation proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cellular component disassembly involved in execution phase of apoptosis cellular response to DNA damage stimulus heart development sensory perception of sound learning or memory activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c response to UV response to glucose response to X-ray regulation of macroautophagy protein processing hippocampus development neuron differentiation keratinocyte differentiation erythrocyte differentiation platelet formation negative regulation of B cell proliferation response to cobalt ion response to estradiol response to lipopolysaccharide glial cell apoptotic process response to tumor necrosis factor response to nicotine hippo signaling wound healing response to drug response to hydrogen peroxide T cell homeostasis negative regulation of apoptotic process response to amino acid positive regulation of neuron apoptotic process cell fate commitment negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of activated T cell proliferation response to antibiotic neurotrophin TRK receptor signaling pathway response to glucocorticoid neuron apoptotic process cellular response to organic cyclic compound apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand execution phase of apoptosis response to wounding intracellular signal transduction positive regulation of apoptotic process positive regulation of catalytic activity negative regulation of cell cycle cellular response to organic substance release of cytochrome c from mitochondria extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to oxidative stress response to organic substance response to metal ion response to organic cyclic compound regulation of apoptotic DNA fragmentation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Caspase 3 Monoclonal Antibody (74T2)

Caspase 3 Antibody (43-7800) in IHC (P)

Product Details

43-7800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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