Invitrogen

Caspase 3 Monoclonal Antibody (CPP32 4-1-18)

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

17 References

View all (56) Caspase 3 antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 17

Caspase 3 Antibody (MA1-16843) in IHC (P)

Immunohistochemical analysis of Caspase 3 in immersion fixed paraffin-embedded sections of human bladder. Samples were incubated in Caspase 3 monoclonal antibody (Product # MA1-16843) using a dilution of 1:300 for 1 hour at room temperature followed by the anti-mouse IgG VisUCyte HRP polymer. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).

Caspase 3 Antibody in Immunohistochemistry (IHC)

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Product Details

MA1-16843

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

2 µg/mL

Immunoprecipitation (IP)

2 µg/ mg lysate

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Insect, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

CPP32 4-1-18

Immunogen

Full-length recombinant human Caspase 3 protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568479

Product Specific Information

Suggested positive control: antigen standard for CASP3 (transient overexpression lysate), HEK 293 whole cell lysate.

Target Information

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats.

Frontiers in nutrition

Long X,Wu H,Zhou Y,Wan Y,Kan X,Gong J,Zhao X

MA1-16843 was used in Western Blotting to show that L. fermentum SCHY34 showed a great biological scavenging and potential effect on alleviating the toxicity of lead ions.

Sat Jul 16 00:00:00 EDT 2022

Moringa oleifera alcoholic extract protected stomach from bisphenol A-induced gastric ulcer in rats via its anti-oxidant and anti-inflammatory activities.

Environmental science and pollution research international

Abo-Elsoud RAEA,Ahmed Mohamed Abdelaziz S,Attia Abd Eldaim M,Hazzaa SM

Published figure using Caspase 3 monoclonal antibody (Product # MA1-16843) in Immunohistochemistry

Thu Sep 01 00:00:00 EDT 2022

Infarct-preconditioning exosomes of umbilical cord mesenchymal stem cells promoted vascular remodeling and neurological recovery after stroke in rats.

Stem cell research & therapy

Ye YC,Chang ZH,Wang P,Wang YW,Liang J,Chen C,Wang JJ,Sun HT,Wang Y,Li XH

MA1-16843 was used in Western Blotting to investigate if exosomes secreted in response to infarction microenvironment could have further therapeutic effects.

Thu Jul 28 00:00:00 EDT 2022

Limonoids from Guarea guidonia and Cedrela odorata: Heat Shock Protein 90 (Hsp90) Modulator Properties of Chisomicine D.

Journal of natural products

Bellone ML,Muñoz Camero C,Chini MG,Dal Piaz F,Hernandez V,Bifulco G,De Tommasi N,Braca A

Published figure using Caspase 3 monoclonal antibody (Product # MA1-16843) in Western Blot

Fri Mar 26 00:00:00 EDT 2021

Recombinant human bone morphogenetic protein 2 and 7 inhibit the degeneration of intervertebral discs by blocking the Puma-dependent apoptotic signaling.

International journal of biological sciences

Xie S,Zhao C,Chen W,Li G,Xiong Z,Tang X,Zhang F,Xiao H

MA1-16843 was used in Western Blot to investigate whether recombinant human bone morphogenetic proteins (rhBMPs) function in the prevention of intervertebral disc degeneration.

Fri Mar 25 00:00:00 EDT 2022

Recombinant human bone morphogenetic protein 2 and 7 inhibit the degeneration of intervertebral discs by blocking the Puma-dependent apoptotic signaling.

International journal of biological sciences

Xie S,Zhao C,Chen W,Li G,Xiong Z,Tang X,Zhang F,Xiao H

MA1-16843 was used in Western Blot to investigate whether recombinant human bone morphogenetic proteins (rhBMPs) function in the prevention of intervertebral disc degeneration.

Fri Mar 25 00:00:00 EDT 2022

Therapeutic effects of syringaldehyde on spinal cord ischemia in rabbits.

Saudi medical journal

Malçok ÜA,Aras AB,Şehitoğlu MH,Akman T,Yüksel Y

MA1-16843 was used in Immunohistochemistry to investigate the effects of syringaldehyde (SA) on the antioxidant and oxidant system in spinal cord ischemia (SCI).

Wed Apr 01 00:00:00 EDT 2020

Therapeutic effects of syringaldehyde on spinal cord ischemia in rabbits.

Saudi medical journal

Malçok ÜA,Aras AB,Şehitoğlu MH,Akman T,Yüksel Y

MA1-16843 was used in Immunohistochemistry to investigate the effects of syringaldehyde (SA) on the antioxidant and oxidant system in spinal cord ischemia (SCI).

Wed Apr 01 00:00:00 EDT 2020

The dual anti-inflammatory and antioxidant activities of natural honey promote cell proliferation and neural regeneration in a rat model of colitis.

Acta histochemica

Nooh HZ,Nour-Eldien NM

MA116843 was used in immunohistochemistry - paraffin section to test the effect of honey in a rat model of ulcerative colitis

Fri Jul 01 00:00:00 EDT 2016

Caspase 3 expression and plasma level of Fas ligand as apoptosis biomarkers in inflammatory endotoxemic lung injury.

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie

Fodor RŞ,Georgescu AM,Grigorescu BL,Cioc AD,Veres M,Cotoi OS,Fodor P,Copotoiu SM,Azamfirei L

MA116843 was used in immunohistochemistry to determine if the immunohistochemical expression of a pulmonary apoptosis marker and plasma level of Fas ligand correlates with the dose- and time-dependent severity of lung injury

Mon May 01 00:00:00 EDT 2017

Single-cell microinjection assay indicates that 7-hydroxycoumarin induces rapid activation of caspase-3 in A549 cancer cells.

Experimental and therapeutic medicine

Soto-Nuñez M,Díaz-Morales KA,Cuautle-Rodríguez P,Torres-Flores V,López-González JS,Mandoki-Weitzner JJ,Molina-Guarneros JA

MA1-16843 was used in western blot to test if 7-hydroxycoumarin induces changes in caspase-3 activity in human lung carcinoma cells

Sun Nov 01 00:00:00 EDT 2015

Single-cell microinjection assay indicates that 7-hydroxycoumarin induces rapid activation of caspase-3 in A549 cancer cells.

Experimental and therapeutic medicine

Soto-Nuñez M,Díaz-Morales KA,Cuautle-Rodríguez P,Torres-Flores V,López-González JS,Mandoki-Weitzner JJ,Molina-Guarneros JA

MA1-16843 was used in western blot to test if 7-hydroxycoumarin induces changes in caspase-3 activity in human lung carcinoma cells

Sun Nov 01 00:00:00 EDT 2015

Apoptosis in the pathogenesis of Nosema ceranae (Microsporidia: Nosematidae) in honey bees (Apis mellifera).

Environmental microbiology reports

Higes M,Juarranz Á,Dias-Almeida J,Lucena S,Botías C,Meana A,García-Palencia P,Martín-Hernández R

MA1-16843 was used in immunohistochemistry - paraffin section to study apoptosis in ventricular cells infected with N. ceranae

Thu Aug 01 00:00:00 EDT 2013

Exendin-4 improves hepatocyte injury by decreasing proliferation through blocking NGF/TrkA in diabetic mice.

Peptides

Gezginci-Oktayoglu S,Sacan O,Yanardag R,Karatug A,Bolkent S

MA1-16843 was used in immunohistochemistry to study the mechanism by which exendin-4 decreases proliferation and improves hepatocyte injury in diabetic mice

Tue Feb 01 00:00:00 EST 2011

Antiapoptotic effect of angiotensin-II type-1 receptor blockade in renal tubular cells of hyperoxaluric rats.

Urological research

Tunçdemir M,Demirkesen O,Oztürk M,Atukeren P,Gümüştaş MK,Turan T

MA1-16843 was used in immunohistochemistry to study the anti-apoptotic effect of an angiotensin-II type-1 receptor blocker in renal epithelial cells of hyperoxaluric rats

Thu Apr 01 00:00:00 EDT 2010

Effect of vitamin C in reducing the toxicity of endosulfan in liver in rabbits.

Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie

Mor F,Ozmen O

MA1-16843 was used in immunohistochemistry to investigate the protective effect of vitamin C from endosulfan toxicity in liver

Fri Jan 01 00:00:00 EST 2010

Effects of Z-FA.FMK on D-galactosamine/tumor necrosis factor-alpha-induced kidney injury and oxidative stress in mice : effects of Z-FA.FMK on TNF-alpha-mediated kidney injury.

Molecular and cellular biochemistry

Gezginci-Oktayoglu S,Tunali S,Yanardag R,Bolkent S

MA1-16843 was used in western blot to examine the protective effect of cathepsin B inhibitor benzyloxicarbonyl-L-phenylalanyl-alanine-fluoromethylketone against kidney injuries

Fri Feb 01 00:00:00 EST 2008

Expression of caspase-3-dependent apoptosis in mural thrombi leukocytes.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Chu PH,Jung SM,Yeh CH,Wu HH,Shiu TF,She HC,Tseng NM

MA1-16843 was used in immunohistochemistry to study caspase-3-dependent apoptosis in mural thrombi leukocytes

Sat Nov 01 00:00:00 EDT 2008

Decreased proliferation precedes growth factor changes after physeal irradiation.

Clinical orthopaedics and related research

Damron TA,Horton JA,Naqvi A,Margulies B,Strauss J,Grant W,Farnum CE,Spadaro JA

MA1-16843 was used in immunohistochemistry to examine the earlier sequential relationship of proliferative, growth factor, and histomorphometric changes after irradiation leading to the 1-week nadir

Sat May 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: Apopain; Apopain precursor; CASP-3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase3; CPP-32; CPP32beta; Cysteine protease CPP32; ICE3; IRP; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; yama protein

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Gene Aliases: A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama

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UniProt ID: (Human) P42574, (Mouse) P70677, (Rat) P55213

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Entrez Gene ID: (Human) 836, (Mouse) 12367, (Rat) 25402

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Function(s)

protease binding aspartic-type endopeptidase activity cysteine-type endopeptidase activity cyclin-dependent protein serine/threonine kinase inhibitor activity death receptor binding protein binding peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process phospholipase A2 activator activity protein complex binding cysteine-type endopeptidase activity involved in apoptotic process cysteine-type endopeptidase activity involved in execution phase of apoptosis cysteine-type peptidase activity hydrolase activity protease

Process(es)

response to hypoxia B cell homeostasis apoptotic DNA fragmentation proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cellular component disassembly involved in execution phase of apoptosis cellular response to DNA damage stimulus heart development sensory perception of sound learning or memory activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c response to UV response to glucose response to X-ray regulation of macroautophagy protein processing hippocampus development neuron differentiation keratinocyte differentiation erythrocyte differentiation platelet formation negative regulation of B cell proliferation response to cobalt ion response to estradiol response to lipopolysaccharide glial cell apoptotic process response to tumor necrosis factor response to nicotine hippo signaling wound healing response to drug response to hydrogen peroxide T cell homeostasis negative regulation of apoptotic process response to amino acid positive regulation of neuron apoptotic process cell fate commitment negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of activated T cell proliferation response to antibiotic neurotrophin TRK receptor signaling pathway response to glucocorticoid neuron apoptotic process cellular response to organic cyclic compound apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand execution phase of apoptosis response to wounding intracellular signal transduction positive regulation of apoptotic process positive regulation of catalytic activity negative regulation of cell cycle cellular response to organic substance release of cytochrome c from mitochondria extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to oxidative stress response to organic substance response to metal ion response to organic cyclic compound regulation of apoptotic DNA fragmentation

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Caspase 3 Monoclonal Antibody (CPP32 4-1-18)

Caspase 3 Antibody (MA1-16843) in IHC (P)

Product Details

MA1-16843

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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