Invitrogen

Caspase 3 (Cleaved Asp175) Polyclonal Antibody

14 Published Figures

18 References

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Caspase 3 (Cleaved Asp175) Antibody in Immunocytochemistry (ICC/IF)

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Caspase 3 (Cleaved Asp175) Antibody (PA5-114687) in ICC/IF

Immunocytochemistry analysis of Caspase 3 (Cleaved Asp175) in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114687) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 ... View More

Caspase 3 (Cleaved Asp175) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Caspase 3 (Cleaved Asp175) Antibody in Western Blot (WB)

Caspase 3 (Cleaved Asp175) Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-114687

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

View 1 publication 1 publication

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CASP3(Accession P42574), corresponding to amino acid residues C163-M182.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2899323

Product Specific Information

Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.

Target Information

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Propofol synergizes with circAPBB2 to protect against hypoxia/reoxygenation-induced oxidative stress, inflammation, and apoptosis of human cardiomyocytes.

Immunity, inflammation and disease

Jin C,Yuan S,Piao L,Ren M,Liu Q

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Western Blot

Tue Aug 01 00:00:00 EDT 2023

CircRANBP17 modulated KDM1A to regulate neuroblastoma progression by sponging miR-27b-3p.

Open medicine (Warsaw, Poland)

Zhao L,Fan J,Zhang C,Zhang Z,Dong J

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Western Blot

Wed Mar 22 00:00:00 EDT 2023

Granulocyte-colony stimulating factor ameliorates di-ethylhexyl phthalate-induced cardiac muscle injury via stem cells recruitment, Desmin protein regulation, antifibrotic and antiapoptotic mechanisms.

Journal of molecular histology

Abdelrahman SA,Khattab MA,Youssef MS,Mahmoud AA

PA5-114687 was used in Immunohistochemistry to examine in depth the effect of DEHP on the histological and biochemical structure of the cardiac muscle in adult male albino rats and the mechanisms underlying the possible ameliorative effect of G-CSF.

Tue Aug 01 00:00:00 EDT 2023

miR-506-3p Relieves Neuropathic Pain following Brachial Plexus Avulsion via Mitigating Microglial Activation through Targeting the CCL2-CCR2 Axis.

Developmental neuroscience

Jin X,Zheng W,Chi S,Cui T,He W

PA5-114687 was used in Immunohistochemistry to show that miR-506-3p had neuroprotective and inflammation-fighting functions in the tBPI rat model via CCL2/CCR2/NF-κB axis.

Fri Mar 10 00:00:00 EST 2023

ADAM metallopeptidase domain 10 knockdown enables podocytes to resist high glucose stimulation by inhibiting pyroptosis via MAPK pathway.

Experimental and therapeutic medicine

Sui C,Zhou D

PA5-114687 was used in Western Blotting to demonstrate that ADAM10 knockdown suppressed the inflammation, apoptosis and pyroptosis of HG-stimulated podocytes by blocking the MAPK signaling pathway.

Thu Jun 01 00:00:00 EDT 2023

miR-340-5p inhibits pancreatic acinar cell inflammation and apoptosis via targeted inhibition of HMGB1.

Experimental and therapeutic medicine

Gao Y,Wang L,Niu Z,Feng H,Liu J,Sun J,Gao Y,Pan L

PA5-114687 was used in Western Blot to present that miR-340-5p may be a useful strategy in AP via upregulation of HMGB1 and subsequent promotion of inflammation and apoptosis.

Tue Feb 01 00:00:00 EST 2022

Purine nucleoside phosphorylase deficiency induces p53-mediated intrinsic apoptosis in human induced pluripotent stem cell-derived neurons.

Scientific reports

Tsui M,Biro J,Chan J,Min W,Dobbs K,Notarangelo LD,Grunebaum E

PA5-114687 was used in Immunocytochemistry-immunoflourescence to establish a potential mechanism for the neurological defects observed in PNP-deficient patients and reinforce the critical role that PNP has for neuronal viability.

Tue May 31 00:00:00 EDT 2022

Purine nucleoside phosphorylase deficiency induces p53-mediated intrinsic apoptosis in human induced pluripotent stem cell-derived neurons.

Scientific reports

Tsui M,Biro J,Chan J,Min W,Dobbs K,Notarangelo LD,Grunebaum E

Tue May 31 00:00:00 EDT 2022

Penta-O-Galloyl-β-D-Glucose in Pistacia integerrima Targets AMPK-ULK1 and ERK/STAT3 Signaling Axes to Induce ROS-Independent Autophagic Cell Death in Human Lung Cancer Cells.

Frontiers in pharmacology

Balkrishna A,Mulay VP,Verma S,Srivastava J,Lochab S,Varshney A

PA5-114687 was used in Immunocytochemistry, Immunohistochemistry (PFA fixed), Western Blot to propose PGG as a potential AMPK activator and STAT3 inhibitor that can be exploited further in developing adjuvant chemotherapeutics against lung cancer.

Sat Aug 06 00:00:00 EDT 2022

Penta-O-Galloyl-β-D-Glucose in Pistacia integerrima Targets AMPK-ULK1 and ERK/STAT3 Signaling Axes to Induce ROS-Independent Autophagic Cell Death in Human Lung Cancer Cells.

Frontiers in pharmacology

Balkrishna A,Mulay VP,Verma S,Srivastava J,Lochab S,Varshney A

Sat Aug 06 00:00:00 EDT 2022

Yi-Shen-Hua-Shi granules inhibit diabetic nephropathy by ameliorating podocyte injury induced by macrophage-derived exosomes.

Frontiers in pharmacology

Liang M,Zhu X,Zhang D,He W,Zhang J,Yuan S,He Q,Jin J

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Immunohistochemistry

Wed Dec 21 00:00:00 EST 2022

Survival of Stem Cells and Progenitors in the Intestine Is Regulated by LPA₅-Dependent Signaling.

Cellular and molecular gastroenterology and hepatology

Liang Z,He P,Han Y,Yun CC

PA5-114687 was used in Immunocytochemistry, Immunohistochemistry (PFA fixed), Western Blot to identify LPA5 receptor as a potent regulator of the survival of stem cells and transit-amplifying cells in the intestine.

Mon Jun 20 00:00:00 EDT 2022

Nickel's Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs.

Toxics

Mortoglou M,Manić L,Buha Djordjevic A,Bulat Z,Đorđević V,Manis K,Valle E,York L,Wallace D,Uysal-Onganer P

PA5-114687 was used in ELISA to indicate that the environmental pollutant Ni plays an important role in the progression of PDAC by promoting an association between miRs and Ni exposure during PDAC pathogenesis.

Mon Mar 21 00:00:00 EDT 2022

Ameliorative Effect of Curcumin Nanoparticles against Monosodium Iodoacetate-Induced Knee Osteoarthritis in Rats.

Mediators of inflammation

Hamdalla HM,Ahmed RR,Galaly SR,Naguib IA,Alghamdi BS,Ahmed OM,Farghali A,Abdul-Hamid M

PA5-114687 was used in Immunocytochemistry, Immunohistochemistry (PFA fixed), Western Blot to demonstrate that CUR NPs provides effective protection against MIA-induced OA and knee joint histological deteriorated changes due to its anti-inflammatory, antioxidant, and antiapoptotic properties.

Fri Dec 30 00:00:00 EST 2022

Pharmacological Disruption of Phosphorylated Eukaryotic Initiation Factor-2α/Activating Transcription Factor 4/Indian Hedgehog Protects Intervertebral Disc Degeneration via Reducing the Reactive Oxygen Species and Apoptosis of Nucleus Pulposus Cells.

Frontiers in cell and developmental biology

Bao J,Qian Z,Liu L,Hong X,Che H,Wu X

PA5-114687 was used in Immunohistochemistry, Western Blot to investigate the impact of phosphorylation of eukaryotic initiation factor-2α (eIF2α)/activating transcription factor 4 (ATF4)/Indian hedgehog (Ihh) signalling in intervertebral disc degeneration.

Fri Jun 25 00:00:00 EDT 2021

Frontiers in cell and developmental biology

Bao J,Qian Z,Liu L,Hong X,Che H,Wu X

Fri Jun 25 00:00:00 EDT 2021

Frontiers in cell and developmental biology

Bao J,Qian Z,Liu L,Hong X,Che H,Wu X

Fri Jun 25 00:00:00 EDT 2021

Frontiers in cell and developmental biology

Bao J,Qian Z,Liu L,Hong X,Che H,Wu X

Fri Jun 25 00:00:00 EDT 2021

LncRNA HOXA11-AS aggravates the keloid formation by targeting miR-148b-3p/IGFBP5 axis.

Biochemical and biophysical research communications

Wang J,Shen J

PA5-114687 was used in Western Blot to show keloid progression can be promoted by long non-coding RNA homeobox HOXA11-AS.

Fri Dec 03 00:00:00 EST 2021

Effects of lncRNA ANRIL-knockdown on the proliferation, apoptosis and cell cycle of gastric cancer cells.

Oncology letters

Hu X,Lou T,Yuan C,Wang Y,Tu X,Wang Y,Zhang T

PA5-114687 was used in Western Blotting to construct drug-resistant gastric cancer cell lines using the stepwise continuous selection method.

Sun Aug 01 00:00:00 EDT 2021

PAD4 inhibitor promotes DNA damage and radiosensitivity of nasopharyngeal carcinoma cells.

Environmental toxicology

Chen H,Wei L,Luo M,Wang X,Zhan Y,Mao Y,Huang C,Li J,Lu H

PA5-114687 was used in Immunocytochemistry, Immunohistochemistry (PFA fixed), Western Blot to determine the effects of YW3-56 on the radiosensitivity of NPC cells.

Mon Nov 01 00:00:00 EDT 2021

PAX8-AS1 knockdown facilitates cell growth and inactivates autophagy in osteoblasts via the miR-1252-5p/GNB1 axis in osteoporosis.

Experimental & molecular medicine

Huang C,Li R,Yang C,Ding R,Li Q,Xie D,Zhang R,Qiu Y

PA5-114687 was used in Western Blotting to determine the specific role of PAX8-AS1 in osteoporosis.

Sat May 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: Apopain; CASP-3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; CPP-32; CPP32beta; Cysteine protease CPP32; IRP; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; Yama; yama protein

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Gene Aliases: A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama

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UniProt ID: (Human) P42574, (Mouse) P70677, (Rat) P55213

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Entrez Gene ID: (Human) 836, (Mouse) 12367, (Rat) 25402

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Function(s)

protease binding aspartic-type endopeptidase activity cysteine-type endopeptidase activity cyclin-dependent protein serine/threonine kinase inhibitor activity death receptor binding protein binding peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process phospholipase A2 activator activity protein complex binding cysteine-type endopeptidase activity involved in apoptotic process cysteine-type endopeptidase activity involved in execution phase of apoptosis cysteine-type peptidase activity hydrolase activity protease

Process(es)

response to hypoxia B cell homeostasis apoptotic DNA fragmentation proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cellular component disassembly involved in execution phase of apoptosis cellular response to DNA damage stimulus heart development sensory perception of sound learning or memory activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c response to UV response to glucose response to X-ray regulation of macroautophagy protein processing hippocampus development neuron differentiation keratinocyte differentiation erythrocyte differentiation platelet formation negative regulation of B cell proliferation response to cobalt ion response to estradiol response to lipopolysaccharide glial cell apoptotic process response to tumor necrosis factor response to nicotine hippo signaling wound healing response to drug response to hydrogen peroxide T cell homeostasis negative regulation of apoptotic process response to amino acid positive regulation of neuron apoptotic process cell fate commitment negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of activated T cell proliferation response to antibiotic neurotrophin TRK receptor signaling pathway response to glucocorticoid neuron apoptotic process cellular response to organic cyclic compound apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand execution phase of apoptosis response to wounding intracellular signal transduction positive regulation of apoptotic process positive regulation of catalytic activity negative regulation of cell cycle cellular response to organic substance release of cytochrome c from mitochondria extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to oxidative stress response to organic substance response to metal ion response to organic cyclic compound regulation of apoptotic DNA fragmentation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Caspase 3 (Cleaved Asp175) Polyclonal Antibody

Caspase 3 (Cleaved Asp175) Antibody (PA5-114687) in ICC/IF

Product Details

PA5-114687

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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