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Caspase 9 (Cleaved Asp330) Antibody (PA5-77889) in WB
Western Blot using Caspase 9 (Cleaved Asp330) Polyclonal Antibody (Product # PA5-77889). Untreated (–) and treated (+) Jurkat whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 9 (Cleaved Asp330) Polyclonal Antibody (Product # PA5-77889) diluted at 1:500.
Product Details
PA5-77889
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Caspase 9 (cleaved Asp330). The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
2.73 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2735576
Product Specific Information
Positive Control: Jurkat (10 µM Etoposide treatment for 24 hr)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Caspase 9 (Cleaved Asp330) encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 25 kDa caspase-9 dominant negative protein; APAF-3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase-9; caspase-9 dominant negative form; caspase-9-carboxyl-terminal divergent; ICE-LAP6; ICE-like apoptotic protease 6; protein phosphatase 1, regulatory subunit 56
Gene Aliases: APAF-3; APAF3; Casp-9-CTD; CASP9; Casp9_v1; ICE-LAP6; MCH6; PPP1R56
UniProt ID: (Human) P55211
Entrez Gene ID: (Human) 842, (Rat) 58918
proteolysis
apoptotic process
cellular response to DNA damage stimulus
aging
intrinsic apoptotic signaling pathway in response to DNA damage
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
platelet formation
response to cobalt ion
response to estradiol
response to lipopolysaccharide
glial cell apoptotic process
cellular response to UV
signal transduction in response to DNA damage
positive regulation of neuron apoptotic process
response to antibiotic
cellular response to dexamethasone stimulus
extrinsic apoptotic signaling pathway in absence of ligand
regulation of response to DNA damage stimulus
activation of cysteine-type endopeptidase activity involved in apoptotic process
response to UV
response to organic cyclic compound
positive regulation of apoptotic process
cellular response to organic cyclic compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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