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Invitrogen
Catalase Recombinant Rabbit Monoclonal Antibody (3H3L29)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
Catalase Antibody (702732) in WB
Western blot analysis was performed on Membrane enriched extracts (30 µg lysate) of MCF-7 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), K-562 (Lane 4), A549 (Lane 5), HL-60 (Lane 6), HEK-293 (Lane 7) and tissue extracts of Mouse Liver (Lane 8). The blots were probed with Anti-Catalase Recombinant Rabbit Monoclonal Antibody (Product # 702732, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 59 kDa band correspon... View More
Product Details
702732
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
3H3L29
Immunogen
Protein corresponding to human Catalse [aa1-aa527]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2716888
Product Specific Information
This antibody predicted to react with Monkey, Bovine, Pig, Sheep
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Catalase is a homotetrameric heme-containing enzyme present within the matrix of all peroxisomes. It carries out a dismutation reaction in which hydrogen peroxide is converted to water and oxygen. Human catalase has the last four amino acids (-KANL) at the extreme C-terminus for peroxisome targeting. The monomer of human catalase is 61.3 kDa in molecular size. Catalase has been implicated as an important factor in inflammation, mutagenesis, prevention of apoptosis, and stimulation of a wide spectrum of tumors. Loss of catalase leads to the human genetic disease, acatalasemia, or Takahara's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Catalase; catalase 1; CTA1; HGNC:1516; MGC138422; MGC138424
Gene Aliases: 2210418N07; Cas-1; Cas1; CAT; Cs-1
UniProt ID: (Human) P04040, (Mouse) P24270
Entrez Gene ID: (Human) 847, (Mouse) 12359
response to reactive oxygen species
osteoblast differentiation
ureteric bud development
response to hypoxia
purine nucleotide catabolic process
triglyceride metabolic process
aging
cholesterol metabolic process
aerobic respiration
response to light intensity
UV protection
response to ozone
response to lead ion
positive regulation of phosphatidylinositol 3-kinase signaling
response to activity
response to inactivity
hemoglobin metabolic process
negative regulation of NF-kappaB transcription factor activity
response to estradiol
response to insulin
response to vitamin A
response to vitamin E
response to L-ascorbic acid
response to drug
response to hydrogen peroxide
hydrogen peroxide catabolic process
negative regulation of apoptotic process
response to ethanol
response to cadmium ion
positive regulation of NF-kappaB transcription factor activity
protein tetramerization
protein homotetramerization
positive regulation of cell division
response to hyperoxia
response to fatty acid
cellular response to growth factor stimulus
response to phenylpropanoid
cellular oxidant detoxification
kidney development
response to oxidative stress
oxidation-reduction process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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