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Cathepsin B Polyclonal Antibody

Cathepsin B Antibody in Immunocytochemistry (ICC/IF)

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Cathepsin B Antibody (12216-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using Cathepsin B antibody (Product # 12216-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Cathepsin B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cathepsin B Antibody in Western Blot (WB)

Cathepsin B Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

12216-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 35 publications 35 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

View 7 publications 7 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cathepsin B Fusion Protein Ag2898 (1-339 aa encoded by BC010240)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MWQLWASLC CLLVLANARS RPSFHPVSDE LVNYVNKRNT TWQAGHNFYN VDMGYLKRLC GTFLGGPKPP QRVMFTEDLK LPASFDAREQ WPQCPTIKEI RDQGSCGSCW AFGAVEAISD RICIHTNAHV SVEVSAEDLL TCCGSMCGDG CNGGYPAEAW NFWTRKGLVS GGLYESHVGC RPYSIPPCEH HVNGSRPPCT GEGDTPKCSK ICEPGYSPTY KQDKHYGYNS YSVSNSEKDI MAEIYKNGPV EGAFSVYSDF LLYKSGVYQH VTGEMMGGHA IRILGWGVEN GTPYWLVANS WNTDWGDNGF FKILRGQDHC GIESEVVAGI PRTDQYWEKI (1-339 aa encoded by BC010240)

Target Information

Cathepsin B is a lysosomal cysteine protease that has been implicated in the pathogenesis of rheumatoid arthritis, muscular dystrophy and tumor metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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International journal of oncology

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Evocalcet Rescues Secondary Hyperparathyroidism-driven Cortical Porosity in CKD Male Rats.

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Hasegawa T,Tokunaga S,Yamamoto T,Sakai M,Hongo H,Kawata T,Amizuka N

Sat Feb 11 00:00:00 EST 2023

De novo lipogenesis fuels adipocyte autophagosome and lysosome membrane dynamics.

Nature communications

Rowland LA,Guilherme A,Henriques F,DiMarzio C,Munroe S,Wetoska N,Kelly M,Reddig K,Hendricks G,Pan M,Han X,Ilkayeva OR,Newgard CB,Czech MP

Mon Mar 13 00:00:00 EDT 2023

Mechanistic Insights into the Role of OPN in Mediating Brain Damage via Triggering Lysosomal Damage in Microglia/Macrophage.

Cells

Gai C,Zhao Y,Xin D,Li T,Cheng Y,Jiang Z,Song Y,Liu D,Wang Z

Thu Mar 09 00:00:00 EST 2023

Mechanistic Insights into the Role of OPN in Mediating Brain Damage via Triggering Lysosomal Damage in Microglia/Macrophage.

Cells

Gai C,Zhao Y,Xin D,Li T,Cheng Y,Jiang Z,Song Y,Liu D,Wang Z

Thu Mar 09 00:00:00 EST 2023

The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study.

BMC cancer

E Y,Yu Q,Sun T,Xue H,Zhao XR,Zheng HC

Thu Jun 08 00:00:00 EDT 2023

The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study.

BMC cancer

E Y,Yu Q,Sun T,Xue H,Zhao XR,Zheng HC

Thu Jun 08 00:00:00 EDT 2023

Mycobacterium tuberculosis resides in lysosome-poor monocyte-derived lung cells during chronic infection.

bioRxiv : the preprint server for biology

Zheng W,Chang IC,Limberis J,Budzik JM,Zha BS,Howard Z,Chen L,Ernst JD

Sun May 28 00:00:00 EDT 2023

Cathepsin B mediates the lysosomal-mitochondrial apoptosis pathway in arsenic-induced microglial cell injury.

Human & experimental toxicology

Zhang Z,Pi R,Jiang Y,Ahmad M,Luo H,Luo J,Yang J,Sun B

Wed May 10 00:00:00 EDT 2023

Cathepsin B mediates the lysosomal-mitochondrial apoptosis pathway in arsenic-induced microglial cell injury.

Human & experimental toxicology

Zhang Z,Pi R,Jiang Y,Ahmad M,Luo H,Luo J,Yang J,Sun B

Wed May 10 00:00:00 EDT 2023

The cell type dependent sorting of CD9- and CD81 to extracellular vesicles can be exploited to convey tumor sensitive cargo to target cells.

Drug delivery

Zuppone S,Zarovni N,Vago R

Fri Dec 01 00:00:00 EST 2023

3, 14, 19-Triacetyl Andrographolide alleviates the cognitive dysfunction of 3 × Tg-AD mice by inducing initiation and promoting degradation process of autophagy.

Phytotherapy research : PTR

Zhang H,Xie X,Shi J,Zhao Q,Luo D,Hao J,Zhou Y,Han G,Pang X

Wed Feb 01 00:00:00 EST 2023

TFEB coordinates autophagy and pyroptosis as hepatotoxicity responses to ZnO nanoparticles.

The Science of the total environment

Pei X,Liu D,Li J,Li L,Ding X,Zhang W,Li Z,Xu G,Li C,Li D

Mon Mar 20 00:00:00 EDT 2023

In addition to its endosomal escape effect, platycodin D also synergizes with ribosomal inactivation protein to induce apoptosis in hepatoma cells through AKT and MAPK signaling pathways.

Chemico-biological interactions

Lei J,Zhao J,Long MY,Cao XW,Wang FJ

Thu Sep 01 00:00:00 EDT 2022

Blockade of Autophagy Prevents the Progression of Hyperuricemic Nephropathy Through Inhibiting NLRP3 Inflammasome-Mediated Pyroptosis.

Frontiers in immunology

Hu Y,Shi Y,Chen H,Tao M,Zhou X,Li J,Ma X,Wang Y,Liu N

Tue May 03 00:00:00 EDT 2022

Polystyrene micro(nano)plastics damage the organelles of RBL-2H3 cells and promote MOAP-1 to induce apoptosis.

Journal of hazardous materials

Liu L,Liu B,Zhang B,Ye Y,Jiang W

Thu Sep 15 00:00:00 EDT 2022

Methylmercury induces lysosomal membrane permeabilization through JNK-activated Bax lysosomal translocation in neuronal cells.

Toxicology letters

Tianji L,Dingbang H,Xiao C,Xiaojing M,Fei Z,Bin W

Tue Mar 01 00:00:00 EST 2022

Cathepsin L-containing exosomes from α-synuclein-activated microglia induce neurotoxicity through the P2X7 receptor.

NPJ Parkinson's disease

Jiang T,Xu C,Gao S,Zhang J,Zheng J,Wu X,Lu Q,Cao L,Yang D,Xu J,Chen X

Thu Oct 06 00:00:00 EDT 2022

Lysosomal dysfunction is associated with NLRP3 inflammasome activation in chronic unpredictable mild stress-induced depressive mice.

Behavioural brain research

Li MM,Wang X,Chen XD,Yang HL,Xu HS,Zhou P,Gao R,Zhang N,Wang J,Jiang L,Liu N

Fri Aug 26 00:00:00 EDT 2022

Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy.

Aging cell

Sun J,Li Y,Yang X,Dong W,Yang J,Hu Q,Zhang C,Fang H,Liu A

Sat Jan 01 00:00:00 EST 2022

Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy.

Aging cell

Sun J,Li Y,Yang X,Dong W,Yang J,Hu Q,Zhang C,Fang H,Liu A

Sat Jan 01 00:00:00 EST 2022

Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.

Redox biology

Ouyang S,Li H,Lou L,Huang Q,Zhang Z,Mo J,Li M,Lu J,Zhu K,Chu Y,Ding W,Zhu J,Lin Z,Zhong L,Wang J,Yue P,Turkson J,Liu P,Wang Y,Zhang X

Wed Jun 01 00:00:00 EDT 2022

1,3-dichloro-2-propanol induced hepatic lipid accumulation by inhibiting autophagy via AKT/mTOR/FOXO1 pathway in mice.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Fan Y,Lu J,Liu J,Zhang R,Yu Z,Guan S

Mon Nov 01 00:00:00 EDT 2021

Cordycepin inhibits cell senescence by ameliorating lysosomal dysfunction and inducing autophagy through the AMPK and mTOR-p70S6K pathway.

FEBS open bio

Zuo SQ,Li C,Liu YL,Tan YH,Wan X,Xu T,Li Q,Wang L,Wu YL,Deng FM,Tang B

Fri Oct 01 00:00:00 EDT 2021

Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.

Microorganisms

Yuan L,Chu B,Chen S,Li Y,Liu N,Zhu Y,Zhou D

Tue Nov 16 00:00:00 EST 2021

Curcumin analogue AI-44 alleviates MSU-induced gouty arthritis in mice via inhibiting cathepsin B-mediated NLRP3 inflammasome activation.

International immunopharmacology

Gu Y,Zhu Y,Deng G,Liu S,Sun Y,Lv W

Thu Apr 01 00:00:00 EDT 2021

Curcumin analogue AI-44 alleviates MSU-induced gouty arthritis in mice via inhibiting cathepsin B-mediated NLRP3 inflammasome activation.

International immunopharmacology

Gu Y,Zhu Y,Deng G,Liu S,Sun Y,Lv W

Thu Apr 01 00:00:00 EDT 2021

MEHP induces pyroptosis and autophagy alternation by cathepsin B activation in INS-1 cells.

Environmental science and pollution research international

Jiang L,Qiu T,Yao X,Chen H,Yao K,Sun X,Yang G,Jiang L,Zhang C,Wang N,Zhang H,Liu X

Wed Dec 01 00:00:00 EST 2021

CTSB Knockdown Inhibits Proliferation and Tumorigenesis in HL-60 Cells.

International journal of medical sciences

Peng S,Yang Q,Li H,Pan Y,Wang J,Hu P,Zhang N

Mon Oct 18 00:00:00 EDT 2021

TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.

Cell death & disease

Hu J,Ding X,Tian S,Chu Y,Liu Z,Li Y,Li X,Wang G,Wang L,Wang Z

Mon Apr 12 00:00:00 EDT 2021

Sodium-Glucose Co-transporter-2 Inhibitor of Dapagliflozin Attenuates Myocardial Ischemia/Reperfusion Injury by Limiting NLRP3 Inflammasome Activation and Modulating Autophagy.

Frontiers in cardiovascular medicine

Yu YW,Que JQ,Liu S,Huang KY,Qian L,Weng YB,Rong FN,Wang L,Zhou YY,Xue YJ,Ji KT

Fri Jan 28 00:00:00 EST 2022

Autophagy Contributes to the Rapamycin-Induced Improvement of Otitis Media.

Frontiers in cellular neuroscience

Xie D,Zhao T,Zhang X,Kui L,Wang Q,Wu Y,Zheng T,Ma P,Zhang Y,Molteni H,Geng R,Yang Y,Li B,Zheng QY

Wed Feb 16 00:00:00 EST 2022

A proteomic approach towards understanding the pathogenesis of Mooren's ulcer.

Experimental eye research

Chi H,Hao W,Qi X,Zhang T,Dong Y,Gao H,Wei C,Shi W

Thu Apr 01 00:00:00 EDT 2021

Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.

Nature

Zhang J,Velmeshev D,Hashimoto K,Huang YH,Hofmann JW,Shi X,Chen J,Leidal AM,Dishart JG,Cahill MK,Kelley KW,Liddelow SA,Seeley WW,Miller BL,Walther TC,Farese RV,Taylor JP,Ullian EM,Huang B,Debnath J,Wittmann T,Kriegstein AR,Huang EJ

Tue Dec 01 00:00:00 EST 2020

Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.

Nature

Tue Dec 01 00:00:00 EST 2020

Cilostazol alleviate nicotine induced cardiomyocytes hypertrophy through modulation of autophagy by CTSB/ROS/p38MAPK/JNK feedback loop.

International journal of biological sciences

Wang SY,Ni X,Hu KQ,Meng FL,Li M,Ma XL,Meng TT,Wu HH,Ge D,Zhao J,Li Y,Su GH

Wed Nov 10 00:00:00 EST 2021

Nicotine induces cardiac toxicity through blocking mitophagic clearance in young adult rat.

Life sciences

Jia G,Meng Z,Liu C,Ma X,Gao J,Liu J,Guo R,Yan Z,Christopher T,Lopez B,Liu W,Dai H,Lau WB,Jiao X,Zhao J,Wang ZX,Cao J,Wang Y

Tue Sep 15 00:00:00 EDT 2020

Heat shock factor 4 regulates lysosome activity by modulating the αB-crystallin-ATP6V1A-mTOR complex in ocular lens.

Biochimica et biophysica acta. General subjects

Cui X,Feng R,Wang J,Du C,Pi X,Chen D,Li J,Li H,Zhang J,Zhang J,Mu H,Zhang F,Liu M,Hu Y

Sun Mar 01 00:00:00 EST 2020

Inhibition of PI3K/mTOR increased the sensitivity of hepatocellular carcinoma cells to cisplatin via interference with mitochondrial-lysosomal crosstalk.

Cell proliferation

Sheng J,Shen L,Sun L,Zhang X,Cui R,Wang L

Wed May 01 00:00:00 EDT 2019

GILT restricts the cellular entry mediated by the envelope glycoproteins of SARS-CoV, Ebola virus and Lassa fever virus.

Emerging microbes & infections

Chen D,Hou Z,Jiang D,Zheng M,Li G,Zhang Y,Li R,Lin H,Chang J,Zeng H,Guo JT,Zhao X

Wed Jan 15 00:00:00 EST 2020

Dietary eicosapentaenoic acid normalizes hippocampal omega-3 and 6 polyunsaturated fatty acid profile, attenuates glial activation and regulates BDNF function in a rodent model of neuroinflammation induced by central interleukin-1β administration.

European journal of nutrition

Dong Y,Xu M,Kalueff AV,Song C

Wed Aug 01 00:00:00 EDT 2018

European journal of nutrition

Dong Y,Xu M,Kalueff AV,Song C

Wed Aug 01 00:00:00 EDT 2018

Alantolactone induces apoptosis and improves chemosensitivity of pancreatic cancer cells by impairment of autophagy-lysosome pathway via targeting TFEB.

Toxicology and applied pharmacology

He R,Shi X,Zhou M,Zhao Y,Pan S,Zhao C,Guo X,Wang M,Li X,Qin R

Mon Oct 01 00:00:00 EDT 2018

Alantolactone induces apoptosis and improves chemosensitivity of pancreatic cancer cells by impairment of autophagy-lysosome pathway via targeting TFEB.

Toxicology and applied pharmacology

He R,Shi X,Zhou M,Zhao Y,Pan S,Zhao C,Guo X,Wang M,Li X,Qin R

Mon Oct 01 00:00:00 EDT 2018

Promotion of autophagosome-lysosome fusion via salvianolic acid A-mediated SIRT1 up-regulation ameliorates alcoholic liver disease.

RSC advances

Shi X,Sun R,Zhao Y,Fu R,Wang R,Zhao H,Wang Z,Tang F,Zhang N,Tian X,Yao J

Wed May 30 00:00:00 EDT 2018

The role of NLRP3-CASP1 in inflammasome-mediated neuroinflammation and autophagy dysfunction in manganese-induced, hippocampal-dependent impairment of learning and memory ability.

Autophagy

Wang D,Zhang J,Jiang W,Cao Z,Zhao F,Cai T,Aschner M,Luo W

Thu May 04 00:00:00 EDT 2017

The role of NLRP3-CASP1 in inflammasome-mediated neuroinflammation and autophagy dysfunction in manganese-induced, hippocampal-dependent impairment of learning and memory ability.

Autophagy

Wang D,Zhang J,Jiang W,Cao Z,Zhao F,Cai T,Aschner M,Luo W

Thu May 04 00:00:00 EDT 2017

Adipocyte-derived exosomes promote lung cancer metastasis by increasing MMP9 activity via transferring MMP3 to lung cancer cells.

Oncotarget

Wang J,Wu Y,Guo J,Fei X,Yu L,Ma S

Tue Oct 10 00:00:00 EDT 2017

Green Tea Polyphenols, Mimicking the Effects of Dietary Restriction, Ameliorate High-Fat Diet-Induced Kidney Injury via Regulating Autophagy Flux.

Nutrients

Xie X,Yi W,Zhang P,Wu N,Yan Q,Yang H,Tian C,Xiang S,Du M,Getachew Assefa E,Zuo X,Ying C

Sun May 14 00:00:00 EDT 2017

Green Tea Polyphenols, Mimicking the Effects of Dietary Restriction, Ameliorate High-Fat Diet-Induced Kidney Injury via Regulating Autophagy Flux.

Nutrients

Xie X,Yi W,Zhang P,Wu N,Yan Q,Yang H,Tian C,Xiang S,Du M,Getachew Assefa E,Zuo X,Ying C

Sun May 14 00:00:00 EDT 2017

A novel acridine derivative, LS-1-10 inhibits autophagic degradation and triggers apoptosis in colon cancer cells.

Cell death & disease

Fu W,Li X,Lu X,Zhang L,Li R,Zhang N,Liu S,Yang X,Wang Y,Zhao Y,Meng X,Zhu WG

Thu Oct 05 00:00:00 EDT 2017

A novel acridine derivative, LS-1-10 inhibits autophagic degradation and triggers apoptosis in colon cancer cells.

Cell death & disease

Fu W,Li X,Lu X,Zhang L,Li R,Zhang N,Liu S,Yang X,Wang Y,Zhao Y,Meng X,Zhu WG

Thu Oct 05 00:00:00 EDT 2017

NDRG1 inhibition sensitizes osteosarcoma cells to combretastatin A-4 through targeting autophagy.

Cell death & disease

Wang H,Li W,Xu J,Zhang T,Zuo D,Zhou Z,Lin B,Wang G,Wang Z,Sun W,Sun M,Chang S,Cai Z,Hua Y

Thu Sep 14 00:00:00 EDT 2017

NDRG1 inhibition sensitizes osteosarcoma cells to combretastatin A-4 through targeting autophagy.

Cell death & disease

Wang H,Li W,Xu J,Zhang T,Zuo D,Zhou Z,Lin B,Wang G,Wang Z,Sun W,Sun M,Chang S,Cai Z,Hua Y

Thu Sep 14 00:00:00 EDT 2017

Expression profile of cathepsins indicates the potential of cathepsins B and D as prognostic factors in breast cancer patients.

Oncology letters

Sun T,Jiang D,Zhang L,Su Q,Mao W,Jiang C

Fri Jan 01 00:00:00 EST 2016

Expression profile of cathepsins indicates the potential of cathepsins B and D as prognostic factors in breast cancer patients.

Oncology letters

Sun T,Jiang D,Zhang L,Su Q,Mao W,Jiang C

Fri Jan 01 00:00:00 EST 2016

Expression of cystatin C and its effect on EC9706 cells in esophageal carcinoma.

International journal of clinical and experimental pathology

Yan Y,Zhou K,Wang L,Zhou Y,Chen X,Fan Q

Wed Oct 05 00:00:00 EDT 2016

Expression of cystatin C and its effect on EC9706 cells in esophageal carcinoma.

International journal of clinical and experimental pathology

Yan Y,Zhou K,Wang L,Zhou Y,Chen X,Fan Q

Wed Oct 05 00:00:00 EDT 2016

Host-derived, pore-forming toxin-like protein and trefoil factor complex protects the host against microbial infection.

Proceedings of the National Academy of Sciences of the United States of America

Xiang Y,Yan C,Guo X,Zhou K,Li S,Gao Q,Wang X,Zhao F,Liu J,Lee WH,Zhang Y

Tue May 06 00:00:00 EDT 2014

Host-derived, pore-forming toxin-like protein and trefoil factor complex protects the host against microbial infection.

Proceedings of the National Academy of Sciences of the United States of America

Xiang Y,Yan C,Guo X,Zhou K,Li S,Gao Q,Wang X,Zhao F,Liu J,Lee WH,Zhang Y

Tue May 06 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: amyloid precursor protein secretase; APP secretase; APPS; Cathepsin B; Cathepsin B1; cysteine protease

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Gene Aliases: APPS; CB; CPSB; CTSB

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UniProt ID: (Human) P07858, (Mouse) P10605

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Entrez Gene ID: (Human) 1508, (Mouse) 13030

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Function(s)

cysteine-type endopeptidase activity serine-type endopeptidase activity protein binding collagen binding peptidase activity cysteine-type peptidase activity kininogen binding peptide binding proteoglycan binding protein self-association endopeptidase activity hydrolase activity protein complex binding cysteine protease

Process(es)

toll-like receptor signaling pathway proteolysis autophagy spermatogenesis skeletal muscle tissue development response to wounding response to glucose response to organic cyclic compound response to amine collagen catabolic process epithelial cell differentiation regulation of apoptotic process response to peptide hormone response to ethanol decidualization viral entry into host cell regulation of catalytic activity proteolysis involved in cellular protein catabolic process negative regulation of cell death response to interleukin-4 cellular response to mechanical stimulus cellular response to thyroid hormone stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cathepsin B Polyclonal Antibody

Cathepsin B Antibody (12216-1-AP) in ICC/IF

Product Details

12216-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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