Invitrogen

Cathepsin B Recombinant Rabbit Monoclonal Antibody (JA11-02)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

3 References

View all (24) Cathepsin B antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Cathepsin B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 14

Cathepsin B Antibody (MA5-32651) in IHC (P)

Immunohistochemical analysis of Cathepsin B of paraffin-embedded Human liver cancer tissue using a Cathepsin-B Monoclonal antibody (Product #MA5-32651). Counter stained with hematoxylin.

Cathepsin B Antibody in Western Blot (WB)

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Product Details

MA5-32651

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JA11-02

Immunogen

Recombinant protein within Human Cathepsin B aa 7-176

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809928

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Cathepsin B is a lysosomal cysteine protease that has been implicated in the pathogenesis of rheumatoid arthritis, muscular dystrophy and tumor metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SARS-CoV-2 Permissive glioblastoma cell line for high throughput antiviral screening.

Antiviral research

Vanhulle E,Stroobants J,Provinciael B,Camps A,Noppen S,Maes P,Vermeire K

Published figure using Cathepsin B recombinant monoclonal antibody (Product # MA5-32651) in Western Blot

Fri Jul 01 00:00:00 EDT 2022

ANP and BNP Exert Anti-Inflammatory Action via NPR-1/cGMP Axis by Interfering with Canonical, Non-Canonical, and Alternative Routes of Inflammasome Activation in Human THP1 Cells.

International journal of molecular sciences

Mezzasoma L,Talesa VN,Romani R,Bellezza I

MA5-32651 was used in Western Blotting to aim to decipher the molecular mechanism underlying NPs effects on THP-1 cells stimulated with lipopolysaccharide (LPS) + ATP. Involvement of cGMP and PKG-I were assessed pre-treating THP-1 cells with the membrane-permeable analogue, 8-Br-cGMP, and the specific inhibitor KT-5823, respectively.

Tue Dec 22 00:00:00 EST 2020

ANP and BNP Exert Anti-Inflammatory Action via NPR-1/cGMP Axis by Interfering with Canonical, Non-Canonical, and Alternative Routes of Inflammasome Activation in Human THP1 Cells.

International journal of molecular sciences

Mezzasoma L,Talesa VN,Romani R,Bellezza I

Tue Dec 22 00:00:00 EST 2020

Extracellular Vesicles from Human Advanced-Stage Prostate Cancer Cells Modify the Inflammatory Response of Microenvironment-Residing Cells.

Cancers

Mezzasoma L,Costanzi E,Scarpelli P,Talesa VN,Bellezza I

MA5-32651 was used in Western Blotting to evaluate the role of extracellular vesicles in the tumour microenvironment.

Fri Aug 30 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: amyloid precursor protein secretase; APP secretase; APPS; Cathepsin B; Cathepsin B1; cysteine protease

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Gene Aliases: APPS; CPSB; CTSB

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UniProt ID: (Human) P07858

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Entrez Gene ID: (Human) 1508

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Function(s)

cysteine-type endopeptidase activity serine-type endopeptidase activity protein binding collagen binding peptidase activity cysteine-type peptidase activity kininogen binding peptide binding proteoglycan binding protein self-association cysteine protease

Process(es)

toll-like receptor signaling pathway proteolysis autophagy spermatogenesis skeletal muscle tissue development response to wounding response to glucose response to organic cyclic compound response to amine collagen catabolic process epithelial cell differentiation regulation of apoptotic process response to peptide hormone response to ethanol decidualization viral entry into host cell regulation of catalytic activity proteolysis involved in cellular protein catabolic process negative regulation of cell death response to interleukin-4 cellular response to mechanical stimulus cellular response to thyroid hormone stimulus

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cathepsin B Recombinant Rabbit Monoclonal Antibody (JA11-02)

Cathepsin B Antibody (MA5-32651) in IHC (P)

Product Details

MA5-32651

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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