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Cathepsin K Antibody (PA5-144672) in WB
Western blot analysis of Cathepsin K in different various lysates. Lane 1: Jurkat cell lysate; Lane 2: K562 cell lysate; Lane 3: A549 cell lysate; Lane 4: U87MG cell lysate; Lane 5: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa. Observed band size: 27 kDa. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Primary antibody Cathepsin K polyclonal antibody (Product # PA5-144672) with a dilution of 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilut... View More
Product Details
PA5-144672
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within Human Cathepsin K aa 101-250/329.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3092356
Product Specific Information
Positive control: Jurkat cell lysate, K562 cell lysate, A549 cell lysate, U87MG cell lysate, A431 cell lysate.
Predicted band size: 37 kDa
Subcellular Location: Lysosome, Secreted, Apical cell membrane.
Target Information
The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cathepsin K; Cathepsin O; cathepsin O1; Cathepsin O2; Cathepsin X; MGC23107
Gene Aliases: CTS02; CTSK; CTSO; CTSO1; CTSO2; PKND; PYCD
UniProt ID: (Human) P43235
Entrez Gene ID: (Human) 1513
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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