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Cathepsin L Antibody (PA5-100424) in ICC/IF
Immunofluorescent analysis of Cathepsin L in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Cathepsin L polyclonal antibody (Product # PA5-100424) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor... View More
Product Details
PA5-100424
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CTSL(Accession P07711), corresponding to amino acid residues F187-K237.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2849935
Product Specific Information
Antibody detects endogenous levels of total Cathepsin L.
Target Information
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cat L; Cathepsin L1; furless; Major excreted protein; MEP; nackt; p39 cysteine proteinase; Procathepsin L
Gene Aliases: 1190035F06Rik; CATL; CTSL; CTSL1; fs; MEP; nkt
UniProt ID: (Human) P07711, (Mouse) P06797
Entrez Gene ID: (Human) 1514, (Mouse) 13039
fibronectin binding
cysteine-type endopeptidase activity
serine-type endopeptidase activity
protein binding
collagen binding
cysteine-type peptidase activity
histone binding
proteoglycan binding
serpin family protein binding
aminopeptidase activity
peptidase activity
hydrolase activity
cysteine-type carboxypeptidase activity
kininogen binding
protein complex binding
peptide binding
cysteine protease
toll-like receptor signaling pathway
adaptive immune response
proteolysis
antigen processing and presentation
antigen processing and presentation of exogenous peptide antigen via MHC class II
extracellular matrix disassembly
collagen catabolic process
proteolysis involved in cellular protein catabolic process
macrophage apoptotic process
cellular response to thyroid hormone stimulus
cell communication
negative regulation of keratinocyte proliferation
protein processing
protein autoprocessing
hair follicle morphogenesis
catagen
decidualization
epithelial tube branching involved in lung morphogenesis
regulation of actin cytoskeleton reorganization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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