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Invitrogen
Caveolin 2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
Caveolin 2 Antibody (PA1-065) in ICC/IF
Immunofluorescence analysis of Caveolin2 was performed using 3T3L-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Caveolin 2 Polyclonal Antibody (Product # PA1-065) at 5µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat... View More
Product Details
PA1-065
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues M(1) G L E T E K A D V Q L F M A D D A Y(19) C of rat CAV2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2244214
Product Specific Information
PA1-065 detects caveolin-2 from rat and mouse tissues tissues. This antibody does not detect caveolin-1 or -3.
PA1-065 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a ~21 kDa protein representing caveolin-2 from rat heart homogenate.
The PA1-065 immunogen is a synthetic peptide corresponding to residues M(1) G L E T E K A D V Q L F M A D D A Y(19) C of rat CAV2. This sequence is completely conserved in mouse, and ~90% conserved in human caveolin-2. The PA1-065 immunizing peptide (Cat. # PEP-080) is available for use in neutralization and control experiments.
Target Information
Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: caveolae; Caveolin-2; MGC12294; Phospho-CAV2
Gene Aliases: AI447843; Cav2
UniProt ID: (Mouse) Q9WVC3, (Rat) Q2IBC5
Entrez Gene ID: (Mouse) 12390, (Rat) 363425
SNARE binding
protein kinase binding
syntaxin binding
protein binding, bridging
mitogen-activated protein kinase kinase kinase binding
D1 dopamine receptor binding
protein complex scaffold
protein homodimerization activity
protein heterodimerization activity
phosphoprotein binding
scaffold protein binding
protein binding
scaffold/adaptor protein
negative regulation of endothelial cell proliferation
positive regulation of endothelial cell proliferation
endocytosis
vesicle fusion
mitochondrion organization
endoplasmic reticulum organization
chemical synaptic transmission
negative regulation of cell proliferation
insulin receptor signaling pathway
vesicle organization
receptor-mediated endocytosis of virus by host cell
negative regulation of transforming growth factor beta receptor signaling pathway
positive regulation of MAPK cascade
positive regulation of GTPase activity
vesicle docking
skeletal muscle fiber development
positive regulation of peptidyl-tyrosine phosphorylation
protein oligomerization
positive regulation of dopamine receptor signaling pathway
caveola assembly
positive regulation of protein localization to nucleus
regulation of mitotic nuclear division
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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