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Cbl-b Antibody (A302-903A) in WB
Detection of human Cbl-b by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cbl-b antibody A302-903A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Cbl-b was also immunoprecipitated by rabbit anti-Cbl-b antibody A302-902A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
Product Details
A302-903A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 932 and 982 of human Cas-Br-M (murine) Ecotropic Retroviral Transforming Sequence b.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
This gene encodes a protein of unknown function. The protein contains a bromodomain, a sequence motif often found in transcriptional coactivators, and localizes to the nucleus in testis and several other cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cas-Br-M (murine) ecotropic retroviral transforming sequence b; Casitas B-lineage lymphoma proto-oncogene b; Cbl proto-oncogene B, E3 ubiquitin protein ligase; Cbl proto-oncogene, E3 ubiquitin protein ligase B; E3 ubiquitin-protein ligase CBL-B; RING finger protein 56; RING-type E3 ubiquitin transferase CBL-B; SH3-binding protein CBL-B; Signal transduction protein CBL-B
Gene Aliases: Cbl-b; CBLB; Nbla00127; RNF56
UniProt ID: (Human) Q13191
Entrez Gene ID: (Human) 868
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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