Proteintech

Cdc20 Polyclonal Antibody

Cdc20 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Cdc20 Antibody (10252-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human urothelial carcinoma tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Cdc20 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10252-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cdc20 Fusion Protein Ag0295 (1-211 aa encoded by BC001088)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAQFAFESD LHSLLQLDAP IPNAPPARWQ RKAKEAAGPA PSPMRAANRS HSAGRTPGRT PGKSSSKVQT TPSKPGGDRY IPHRSAAQME VASFLLSKEN QPENSQTPTK KEHQKAWALN LNGFDVEEAK ILRLSGKPQN APEGYQNRLK VLYSQKATPG SSRKTCRYIP SLPDRILDAP EIRNDYYLNL VDWSSGNVLA VALDNSVYLW SA (1-211 aa encoded by BC001088)

Target Information

Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CCT6A is associated with CDC20, Enneking stage and prognosis in osteosarcoma, and its knockdown suppresses osteosarcoma cell viability and invasion.

Oncology letters

Wang N,Chen Y,Wu J

Mon May 01 00:00:00 EDT 2023

CCT6A is associated with CDC20, Enneking stage and prognosis in osteosarcoma, and its knockdown suppresses osteosarcoma cell viability and invasion.

Oncology letters

Wang N,Chen Y,Wu J

Mon May 01 00:00:00 EDT 2023

KIF2C Facilitates Tumor Growth and Metastasis in Pancreatic Ductal Adenocarcinoma.

Cancers

Huang X,Zhao F,Wu Q,Wang Z,Ren H,Zhang Q,Wang Z,Xu J

Mon Feb 27 00:00:00 EST 2023

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation.

Nature communications

Xu X,Chang CW,Li M,Omabe K,Le N,Chen YH,Liang F,Liu Y

Sat Mar 04 00:00:00 EST 2023

p53 directly downregulates the expression of CDC20 to exert anti-tumor activity in mantle cell lymphoma.

Experimental hematology & oncology

Chen Y,Yang P,Wang J,Gao S,Xiao S,Zhang W,Zhu M,Wang Y,Ke X,Jing H

Tue Mar 07 00:00:00 EST 2023

p53 directly downregulates the expression of CDC20 to exert anti-tumor activity in mantle cell lymphoma.

Experimental hematology & oncology

Chen Y,Yang P,Wang J,Gao S,Xiao S,Zhang W,Zhu M,Wang Y,Ke X,Jing H

Tue Mar 07 00:00:00 EST 2023

p53 directly downregulates the expression of CDC20 to exert anti-tumor activity in mantle cell lymphoma.

Experimental hematology & oncology

Chen Y,Yang P,Wang J,Gao S,Xiao S,Zhang W,Zhu M,Wang Y,Ke X,Jing H

Tue Mar 07 00:00:00 EST 2023

p53 directly downregulates the expression of CDC20 to exert anti-tumor activity in mantle cell lymphoma.

Experimental hematology & oncology

Chen Y,Yang P,Wang J,Gao S,Xiao S,Zhang W,Zhu M,Wang Y,Ke X,Jing H

Tue Mar 07 00:00:00 EST 2023

N-glycosylation of GDF15 abolishes its inhibitory effect on EGFR in AR inhibitor-resistant prostate cancer cells.

Cell death & disease

Wang R,Wen P,Yang G,Feng Y,Mi Y,Wang X,Zhu S,Chen YQ

Tue Jul 19 00:00:00 EDT 2022

The bioinformatics and experimental analysis of the novel roles of virus infection-associated gene CDC20 for prognosis and immune infiltration in hepatocellular carcinoma.

Aging

Li J,Zhang X,Yao L,Hu K

Fri May 27 00:00:00 EDT 2022

Transcription factor Meis1 act as a new regulator of ischemic arrhythmias in mice.

Journal of advanced research

Liu Y,Li J,Xu N,Yu H,Gong L,Li Q,Yang Z,Li S,Yang J,Huang D,Xue Y,Xue G,Liu J,Chen H,Zhang R,Li A,Zhao Y,Li P,Li M,Liu M,Wang N,Cai B

Fri Jul 01 00:00:00 EDT 2022

TORC1 inactivation promotes APC/C-dependent mitotic slippage in yeast and human cells.

iScience

Yamada C,Morooka A,Miyazaki S,Nagai M,Mase S,Iemura K,Tasnin MN,Takuma T,Nakamura S,Morshed S,Koike N,Mostofa MG,Rahman MA,Sharmin T,Katsuta H,Ohara K,Tanaka K,Ushimaru T

Fri Feb 18 00:00:00 EST 2022

CDCA3 Predicts Poor Prognosis and Affects CD8⁺ T Cell Infiltration in Renal Cell Carcinoma.

Journal of oncology

Bai Y,Liao S,Yin Z,You B,Lu D,Chen Y,Chen D,Wu Y

Tue Oct 11 00:00:00 EDT 2022

The Prognostic Assessment of CDC20 in Patients with Renal Clear Cell Carcinoma and Its Relationship with Body Immunity.

Contrast media & molecular imaging

Shi J,Chen Y,Gu X,Wang X,Liu J,Chen X

Mon Jul 11 00:00:00 EDT 2022

Identification and verification of hub genes associated with the progression of non-small cell lung cancer by integrated analysis.

Frontiers in pharmacology

Mengyan X,Kun D,Xinming J,Yutian W,Yongqian S

Sat Oct 01 00:00:00 EDT 2022

Identification and verification of hub genes associated with the progression of non-small cell lung cancer by integrated analysis.

Frontiers in pharmacology

Mengyan X,Kun D,Xinming J,Yutian W,Yongqian S

Sat Oct 01 00:00:00 EDT 2022

Comparison of the major cell populations among osteoarthritis, Kashin-Beck disease and healthy chondrocytes by single-cell RNA-seq analysis.

Cell death & disease

Wang X,Ning Y,Zhang P,Poulet B,Huang R,Gong Y,Hu M,Li C,Zhou R,Lammi MJ,Guo X

Thu May 27 00:00:00 EDT 2021

The Oncogenic Role of APC/C Activator Protein Cdc20 by an Integrated Pan-Cancer Analysis in Human Tumors.

Frontiers in oncology

Wu F,Sun Y,Chen J,Li H,Yao K,Liu Y,Liu Q,Lu J

Fri Sep 17 00:00:00 EDT 2021

CDC20 regulates the cell proliferation and radiosensitivity of P53 mutant HCC cells through the Bcl-2/Bax pathway.

International journal of biological sciences

Zhao S,Zhang Y,Lu X,Ding H,Han B,Song X,Miao H,Cui X,Wei S,Liu W,Chen S,Wang J

Tue Feb 22 00:00:00 EST 2022

Ursolic Acid Regulates Cell Cycle and Proliferation in Colon Adenocarcinoma by Suppressing Cyclin B1.

Frontiers in pharmacology

Yang M,Hu C,Cao Y,Liang W,Yang X,Xiao T

Fri Feb 26 00:00:00 EST 2021

Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics.

bioRxiv : the preprint server for biology

Wang L,Muneer A,Xie L,Zhang F,Wu B,Mei L,Lenarcic EM,Feng EH,Song J,Xiong Y,Yu X,Wang C,Gheorghe C,Torralba K,Cook JG,Wan YY,Moorman NJ,Song H,Jin J,Chen X

Mon Nov 16 00:00:00 EST 2020

Anaphase-promoting complex/cyclosome-Cdc-20 promotes Zwint-1 degradation.

Cell biochemistry and function

He Y,Li R,Gu L,Deng H,Zhao Y,Guo Y,Yu S,Wang G

Mon Jun 01 00:00:00 EDT 2020

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

Molecular cell

Marmor-Kollet H,Siany A,Kedersha N,Knafo N,Rivkin N,Danino YM,Moens TG,Olender T,Sheban D,Cohen N,Dadosh T,Addadi Y,Ravid R,Eitan C,Toth Cohen B,Hofmann S,Riggs CL,Advani VM,Higginbottom A,Cooper-Knock J,Hanna JH,Merbl Y,Van Den Bosch L,Anderson P,Ivanov P,Geiger T,Hornstein E

Thu Dec 03 00:00:00 EST 2020

Downregulation of CDC20 Increases Radiosensitivity through Mcl-1/p-Chk1-Mediated DNA Damage and Apoptosis in Tumor Cells.

International journal of molecular sciences

Gao Y,Wen P,Chen B,Hu G,Wu L,Xu A,Zhao G

Sat Sep 12 00:00:00 EDT 2020

Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit.

eLife

Holder J,Mohammed S,Barr FA

Tue Sep 01 00:00:00 EDT 2020

CDC20 Knockdown and Acidic Microenvironment Collaboratively Promote Tumorigenesis through Inhibiting Autophagy and Apoptosis.

Molecular therapy oncolytics

Gu Q,Li F,Ge S,Zhang F,Jia R,Fan X

Fri Jun 26 00:00:00 EDT 2020

Identification of biomarkers for the transition from low-grade glioma to secondary glioblastoma by an integrated bioinformatic analysis.

American journal of translational research

Zhao L,Zhang J,Liu Z,Zhao P

Mon Sep 28 00:00:00 EDT 2020

Translation control of the immune checkpoint in cancer and its therapeutic targeting.

Nature medicine

Xu Y,Poggio M,Jin HY,Shi Z,Forester CM,Wang Y,Stumpf CR,Xue L,Devericks E,So L,Nguyen HG,Griselin A,Gordan JD,Umetsu SE,Reich SH,Worland ST,Asthana S,Barna M,Webster KR,Cunningham JT,Ruggero D

Fri Feb 01 00:00:00 EST 2019

Translation control of the immune checkpoint in cancer and its therapeutic targeting.

Nature medicine

Xu Y,Poggio M,Jin HY,Shi Z,Forester CM,Wang Y,Stumpf CR,Xue L,Devericks E,So L,Nguyen HG,Griselin A,Gordan JD,Umetsu SE,Reich SH,Worland ST,Asthana S,Barna M,Webster KR,Cunningham JT,Ruggero D

Fri Feb 01 00:00:00 EST 2019

Upregulation of kinesin family member 4A enhanced cell proliferation via activation of Akt signaling and predicted a poor prognosis in hepatocellular carcinoma.

Cell death & disease

Huang Y,Wang H,Lian Y,Wu X,Zhou L,Wang J,Deng M,Huang Y

Fri Feb 02 00:00:00 EST 2018

Upregulation of kinesin family member 4A enhanced cell proliferation via activation of Akt signaling and predicted a poor prognosis in hepatocellular carcinoma.

Cell death & disease

Huang Y,Wang H,Lian Y,Wu X,Zhou L,Wang J,Deng M,Huang Y

Fri Feb 02 00:00:00 EST 2018

Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis.

Scientific reports

Ikeda M,Tanaka K

Fri Aug 18 00:00:00 EDT 2017

Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis.

Scientific reports

Ikeda M,Tanaka K

Fri Aug 18 00:00:00 EDT 2017

Evaluation of the Antitumor Efficacy of RNAi-Mediated Inhibition of CDC20 and Heparanase in an Orthotopic Liver Tumor Model.

Cancer biotherapy & radiopharmaceuticals

Liu M,Zhang Y,Liao Y,Chen Y,Pan Y,Tian H,Zhan Y,Liu D

Sat Aug 01 00:00:00 EDT 2015

Evaluation of the Antitumor Efficacy of RNAi-Mediated Inhibition of CDC20 and Heparanase in an Orthotopic Liver Tumor Model.

Cancer biotherapy & radiopharmaceuticals

Liu M,Zhang Y,Liao Y,Chen Y,Pan Y,Tian H,Zhan Y,Liu D

Sat Aug 01 00:00:00 EDT 2015

Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine

Wu J,Chen W,Yin Y,Zheng Z,Zou G

Sun Jun 01 00:00:00 EDT 2014

Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine

Wu J,Chen W,Yin Y,Zheng Z,Zou G

Sun Jun 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CDC20 cell division cycle 20 homolog; cell division cycle 20 homolog; Cell division cycle protein 20 homolog; p55CDC

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Gene Aliases: bA276H19.3; CDC20; CDC20A; p55CDC

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UniProt ID: (Human) Q12834

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Entrez Gene ID: (Human) 991

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Function(s)

protein binding protein C-terminus binding anaphase-promoting complex binding enzyme binding histone deacetylase binding ubiquitin-protein transferase activator activity ubiquitin-protein ligase

Process(es)

cell cycle sister chromatid cohesion mitotic nuclear division positive regulation of cell proliferation anaphase-promoting complex-dependent catabolic process positive regulation of synaptic plasticity regulation of meiotic nuclear division protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process regulation of dendrite development cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of synapse maturation positive regulation of ubiquitin protein ligase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cdc20 Polyclonal Antibody

Cdc20 Antibody (10252-1-AP) in IHC (P)

Product Details

10252-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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