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ChAT Antibody (OSC00326W-100UL) in IHC (P)
IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen fro... View More
Product Details
OSC00326W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from mouse ChAT conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
The peptide is homologous in rat and human.
Specificity of this antibody: ChAT.
Target Information
ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetyl CoA; acetyl CoA:choline O-acetyltransferase; CHOACTase; choline acetylase; choline acetyltransferase; Choline O-acetyltransferase
Gene Aliases: B230380D24Rik; CHAT; CHOACTASE; CMS1A; CMS1A2; CMS6
UniProt ID: (Human) P28329
Entrez Gene ID: (Human) 1103, (Rat) 290567, (Mouse) 12647
antral ovarian follicle growth
response to hypoxia
phosphatidylcholine biosynthetic process
neurotransmitter secretion
response to nutrient
memory
acetylcholine biosynthetic process
response to drug
response to ethanol
chemical synaptic transmission
neuromuscular synaptic transmission
muscle organ development
establishment of synaptic specificity at neuromuscular junction
rhythmic behavior
adult walking behavior
metabolic process
dendrite development
neuron differentiation
neurotransmitter biosynthetic process
rhythmic excitation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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