Invitrogen

ChAT Polyclonal Antibody

ChAT Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

ChAT Antibody (PA1-9027) in ICC/IF

Immunofluorescence analysis of ChAT was performed using Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ChAT Polyclonal Antibody (Product # PA1-9027) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Rabbit anti-Goat IgG Heavy Chain, Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27012) (1:2000 dilution) for 45 mi... View More

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Product Details

PA1-9027

Applications

Tested Dilution

Publications

Western Blot (WB)

0.03-0.3 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the C terminus amino acids CKEKATRPSQGHQP.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2079590

Product Specific Information

This antibody is predicted to react with bovine, canine and porcine based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

Target Information

ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genome-wide association study identifies five new susceptibility loci for primary angle closure glaucoma.

Nature genetics

Khor CC,Do T,Jia H,Nakano M,George R,Abu-Amero K,Duvesh R,Chen LJ,Li Z,Nongpiur ME,Perera SA,Qiao C,Wong HT,Sakai H,Barbosa de Melo M,Lee MC,Chan AS,Azhany Y,Dao TL,Ikeda Y,Perez-Grossmann RA,Zarnowski T,Day AC,Jonas JB,Tam PO,Tran TA,Ayub H,Akhtar F,Micheal S,Chew PT,Aljasim LA,Dada T,Luu TT,Awadalla MS,Kitnarong N,Wanichwecharungruang B,Aung YY,Mohamed-Noor J,Vijayan S,Sarangapani S,Husain R,Jap A,Baskaran M,Goh D,Su DH,Wang H,Yong VK,Yip LW,Trinh TB,Makornwattana M,Nguyen TT,Leuenberger EU,Park KH,Wiyogo WA,Kumar RS,Tello C,Kurimoto Y,Thapa SS,Pathanapitoon K,Salmon JF,Sohn YH,Fea A,Ozaki M,Lai JS,Tantisevi V,Khaing CC,Mizoguchi T,Nakano S,Kim CY,Tang G,Fan S,Wu R,Meng H,Nguyen TT,Tran TD,Ueno M,Martinez JM,Ramli N,Aung YM,Reyes RD,Vernon SA,Fang SK,Xie Z,Chen XY,Foo JN,Sim KS,Wong TT,Quek DT,Venkatesh R,Kavitha S,Krishnadas SR,Soumittra N,Shantha B,Lim BA,Ogle J,de Vasconcellos JP,Costa VP,Abe RY,de Souza BB,Sng CC,Aquino MC,Kosior-Jarecka E,Fong GB,Tamanaja VC,Fujita R,Jiang Y,Waseem N,Low S,Pham HN,Al-Shahwan S,Craven ER,Khan MI,Dada R,Mohanty K,Faiq MA,Hewitt AW,Burdon KP,Gan EH,Prutthipongsit A,Patthanathamrongkasem T,Catacutan MA,Felarca IR,Liao CS,Rusmayani E,Istiantoro VW,Consolandi G,Pignata G,Lavia C,Rojanapongpun P,Mangkornkanokpong L,Chansangpetch S,Chan JC,Choy BN,Shum JW,Than HM,Oo KT,Han AT,Yong VH,Ng XY,Goh SR,Chong YF,Hibberd ML,Seielstad M,Png E,Dunstan SJ,Chau NV,Bei J,Zeng YX,Karkey A,Basnyat B,Pasutto F,Paoli D,Frezzotti P,Wang JJ,Mitchell P,Fingert JH,Allingham RR,Hauser MA,Lim ST,Chew SH,Ebstein RP,Sakuntabhai A,Park KH,Ahn J,Boland G,Snippe H,Stead R,Quino R,Zaw SN,Lukasik U,Shetty R,Zahari M,Bae HW,Oo NL,Kubota T,Manassakorn A,Ho WL,Dallorto L,Hwang YH,Kiire CA,Kuroda M,Djamal ZE,Peregrino JI,Ghosh A,Jeoung JW,Hoan TS,Srisamran N,Sandragasu T,Set SH,Doan VH,Bhattacharya SS,Ho CL,Tan DT,Sihota R,Loon SC,Mori K,Kinoshita S,Hollander AI,Qamar R,Wang YX,Teo YY,Tai ES,Hartleben-Matkin C,Lozano-Giral D,Saw SM,Cheng CY,Zenteno JC,Pang CP,Bui HT,Hee O,Craig JE,Edward DP,Yonahara M,Neto JM,Guevara-Fujita ML,Xu L,Ritch R,Liza-Sharmini AT,Wong TY,Al-Obeidan S,Do NH,Sundaresan P,Tham CC,Foster PJ,Vijaya L,Tashiro K,Vithana EN,Wang N,Aung T

PA1-9027 was used in western blot to identify five new susceptibility loci for primary angle closure glaucoma through a genome-wide assocation study

Sun May 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: acetyl CoA; acetyl CoA:choline O-acetyltransferase; CHOACTase; choline acetylase; Choline O-acetyltransferase

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Gene Aliases: CHAT; CHOACTASE; CMS1A; CMS1A2; CMS6

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UniProt ID: (Human) P28329

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Entrez Gene ID: (Human) 1103

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Function(s)

choline O-acetyltransferase activity choline binding acyltransferase

Process(es)

antral ovarian follicle growth response to hypoxia phosphatidylcholine biosynthetic process neurotransmitter secretion response to nutrient memory acetylcholine biosynthetic process response to drug response to ethanol

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ChAT Polyclonal Antibody

ChAT Antibody (PA1-9027) in ICC/IF

Product Details

PA1-9027

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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