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ChAT Antibody (PA5-18518) in WB
Western blot was performed using Anti-ChAT Polyclonal Antibody (Product # PA5-18518) and a 74 kDa band corresponding to ChAT was observed across all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), Neuro-2a (Lane 2), IMR32 (Lane 3), Caco-2 (Lane 4), HCT-116 (Lane 5), HT-29 (Lane 6) and tissue extracts of Mouse Colon (Lane 7), Mouse Brain (Lane 8), Mouse Placenta (Lane 9), and Rat Brain (Lane 10) were electrophoresed using NuPAGE™ NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved prot... View More
Product Details
PA5-18518
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (KERARGPTKPSNLD) corresponding to the C-terminus amino acids of Chat
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_10980568
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
Target Information
ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetyl CoA; CHOACTase; choline acetylase; choline acetyltransferase; choline O-acetyltransferase
Gene Aliases: B230380D24Rik
Entrez Gene ID: (Rat) 290567, (Mouse) 12647
antral ovarian follicle growth
response to hypoxia
chemical synaptic transmission
neuromuscular synaptic transmission
muscle organ development
establishment of synaptic specificity at neuromuscular junction
response to nutrient
memory
rhythmic behavior
adult walking behavior
metabolic process
acetylcholine biosynthetic process
dendrite development
neuron differentiation
neurotransmitter biosynthetic process
response to drug
rhythmic excitation
response to ethanol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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