Invitrogen

ChAT Polyclonal Antibody

ChAT Antibody in Immunocytochemistry (ICC/IF)

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ChAT Antibody (PA5-29653) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of ChAT was performed in DIV14 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: ChAT Polyclonal Antibody (Product # PA5-29653) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

ChAT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ChAT Antibody in Immunohistochemistry (Frozen) (IHC (F))

ChAT Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-29653

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 102 and 351 of Human CHAT

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2547128

Product Specific Information

Recommended positive controls: IMR32, mouse brain, rat brain, DDDDK-tagged CHAT-transfected 293T.

Predicted reactivity: Mouse (93%), Rat (92%), Pig (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Focal impaired awareness seizures in a rodent model: A functional anatomy.

Epilepsia open

Adotevi N,Kapur J

PA5-29653 was used in Immunohistochemistry to highlight neuronal circuits preferentially activated during a focal awareness impaired seizure in a rodent model.

Tue Mar 01 00:00:00 EST 2022

Focal impaired awareness seizures in a rodent model: A functional anatomy.

Epilepsia open

Adotevi N,Kapur J

PA5-29653 was used in Immunohistochemistry to highlight neuronal circuits preferentially activated during a focal awareness impaired seizure in a rodent model.

Tue Mar 01 00:00:00 EST 2022

Targeting the tamoxifen receptor within sodium channels to block osteoarthritic pain.

Cell reports

McCollum MM,Larmore M,Ishihara S,Ng LCT,Kimura LF,Guadarrama E,Ta MC,Vien TN,Frost GB,Scheidt KA,Miller RE,DeCaen PG

PA5-29653 was used in Functional Assay to show that when evaluated using a mouse OA pain model, ND-Tam has long-lasting efficacy, which supports the potential of repurposing ND-Tam analogs as NaV antagonists for OA pain management.

Tue Aug 23 00:00:00 EDT 2022

Pegylated Insulin-Like Growth Factor 1 attenuates Hair Cell Loss and promotes Presynaptic Maintenance of Medial Olivocochlear Cholinergic Fibers in the Cochlea of the Progressive Motor Neuropathy Mouse.

Frontiers in neurology

Bieniussa L,Kahraman B,Skornicka J,Schulte A,Voelker J,Jablonka S,Hagen R,Rak K

PA5-29653 was used in Immunohistochemistry to investigate the effect of peg-IGF-1 on the auditory system by treatment starting at postnatal day 15 (p15).

Sat Jul 16 00:00:00 EDT 2022

Angiopoietin-1 Knockout Mice as a Genetic Model of Open-Angle Glaucoma.

Translational vision science & technology

Thomson BR,Grannonico M,Liu F,Liu M,Mendapara P,Xu Y,Liu X,Quaggin SE

PA5-29653 was used in Immunohistochemistry to develop and characterise Angpt1 conditional knockout mice.

Sun Mar 01 00:00:00 EST 2020

Angiopoietin-1 Knockout Mice as a Genetic Model of Open-Angle Glaucoma.

Translational vision science & technology

Thomson BR,Grannonico M,Liu F,Liu M,Mendapara P,Xu Y,Liu X,Quaggin SE

PA5-29653 was used in Immunohistochemistry to develop and characterise Angpt1 conditional knockout mice.

Sun Mar 01 00:00:00 EST 2020

Bioinformatics

Protein Aliases: acetyl CoA; acetyl CoA:choline O-acetyltransferase; CHOACTase; choline acetylase; choline acetyltransferase; Choline O-acetyltransferase

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Gene Aliases: B230380D24Rik; CHAT; CHOACTASE; CMS1A; CMS1A2; CMS6

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UniProt ID: (Human) P28329

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Entrez Gene ID: (Human) 1103, (Rat) 290567, (Mouse) 12647

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Function(s)

choline O-acetyltransferase activity choline binding transferase activity transferase activity, transferring acyl groups acyltransferase

Process(es)

antral ovarian follicle growth response to hypoxia phosphatidylcholine biosynthetic process neurotransmitter secretion response to nutrient memory acetylcholine biosynthetic process response to drug response to ethanol chemical synaptic transmission neuromuscular synaptic transmission muscle organ development establishment of synaptic specificity at neuromuscular junction rhythmic behavior adult walking behavior metabolic process dendrite development neuron differentiation neurotransmitter biosynthetic process rhythmic excitation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ChAT Polyclonal Antibody

ChAT Antibody (PA5-29653) in ICC/IF

Product Details

PA5-29653

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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