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ChAT Antibody (PA5-77838) in ICC/IF
Immunofluorescent analysis of ChAT in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ChAT polyclonal antibody (Product # PA5-77838) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain B) Phalloidin Texas Red F-Actin stain C) Choline Acetyltransferase Antibody D) Merged image... View More
Product Details
PA5-77838
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2735637
Product Specific Information
A 1:1,000 dilution of PA5-77838 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.|Predicted molecular weight at approximately 82.5kDa. Observed molecular weights between 68-70kDa.
Target Information
ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acetyl CoA; acetyl CoA:choline O-acetyltransferase; CHOACTase; choline acetylase; Choline O-acetyltransferase
Gene Aliases: B230380D24Rik; CHAT; CHOACTASE; CMS1A; CMS1A2; CMS6
UniProt ID: (Human) P28329
Entrez Gene ID: (Human) 1103, (Mouse) 12647
antral ovarian follicle growth
response to hypoxia
phosphatidylcholine biosynthetic process
neurotransmitter secretion
response to nutrient
memory
acetylcholine biosynthetic process
response to drug
response to ethanol
chemical synaptic transmission
neuromuscular synaptic transmission
muscle organ development
establishment of synaptic specificity at neuromuscular junction
rhythmic behavior
adult walking behavior
dendrite development
neuron differentiation
neurotransmitter biosynthetic process
rhythmic excitation
Performance Guarantee
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