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Invitrogen
ChAT Monoclonal Antibody (CL3173)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
ChAT Antibody (MA5-31383) in WB
Western blot was performed using Anti-ChAT Monoclonal Antibody (CL3173) (Product # MA5-31383) and a ~70 kDa band corresponding to acetyl CoA: choline O-acetyltransferase was observed in Mouse and Rat Brain lysates but not in Mouse and Rat Kidney lysates. Whole cell extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Kidney (Lane 3) and Rat Kidney (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane... View More
Product Details
MA5-31383
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
CL3173
Immunogen
Recombinant protein corresponding to Human CHAT
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2787020
Product Specific Information
Immunogen sequence: GLFSSYRLPG HTQDTLVAQN SSIMPEPEHV IVACCNQFFV LDVVINFRRL SEGDLFTQLR KIVKMASNED ERLPPIGLLT SDGRSEWAEA RTVLVKDSTN
Target Information
ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetyl CoA; acetyl CoA:choline O-acetyltransferase; CHOACTase; choline acetylase; choline acetyltransferase; Choline O-acetyltransferase
Gene Aliases: B230380D24Rik; CHAT; CHOACTASE; CMS1A; CMS1A2; CMS6
UniProt ID: (Human) P28329
Entrez Gene ID: (Human) 1103, (Rat) 290567, (Mouse) 12647
antral ovarian follicle growth
response to hypoxia
phosphatidylcholine biosynthetic process
neurotransmitter secretion
response to nutrient
memory
acetylcholine biosynthetic process
response to drug
response to ethanol
chemical synaptic transmission
neuromuscular synaptic transmission
muscle organ development
establishment of synaptic specificity at neuromuscular junction
rhythmic behavior
adult walking behavior
metabolic process
dendrite development
neuron differentiation
neurotransmitter biosynthetic process
rhythmic excitation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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