Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Chicken anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
FIGURE: 1 / 1
Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A15981) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected by chemiluminescence using Chicken anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # A15981) at dilutions 1:500 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 22 kDa band corresponding to ... View More
Product Details
A15981
Applications
Tested Dilution
Western Blot (WB)
1:500-1:20,000
Immunohistochemistry (IHC)
1:500-1:20,000
ELISA (ELISA)
1:500-1:20,000
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobins Heavy and Light chains
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.1% ProClin 150
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2534655
Target
IgG
Cross Adsorption
Against bovine, horse and human serum proteins
Antibody Form
Whole Antibody
Product Specific Information
Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid. Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum immunoglobulins, IgG/serum from human or rabbit.
Target Information
Mouse IgG is an immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant immunoglobulin in the serum and plays a crucial role in the adaptive immune response. Mouse IgG is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. Mouse IgG is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support