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Invitrogen
Chicken anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, TRITC
FIGURE: 1 / 1
Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A15986) in ICC/IF
Immunofluorescence analysis of Chicken anti-Mouse IgG (H+L) Secondary Antibody, TRITC was performed using HeLa cells stained with alpha Tubulin (23610501) Mouse Monoclonal Primary Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Mouse primary antibody (1:250 dilutio... View More
Product Details
A15986
Applications
Tested Dilution
Immunocytochemistry (ICC/IF)
0.5 µg/mL
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobins Heavy and Light chains
Conjugate
Excitation/Emission Max
552/578 nm
View spectra
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2534660
Target
IgG
Cross Adsorption
Against bovine, horse and human serum proteins
Antibody Form
Whole Antibody
Product Specific Information
Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. 1 year from date of receipt. Prepare working dilution prior to use and then discard. Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum immunoglobulins, IgG/serum from human or rabbit.
Target Information
Mouse IgG is an immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant immunoglobulin in the serum and plays a crucial role in the adaptive immune response. Mouse IgG is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. Mouse IgG is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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