Proteintech

Chk1 Polyclonal Antibody

Chk1 Antibody in Immunocytochemistry (ICC/IF)

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Chk1 Antibody (25887-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293T cells using Chk1 antibody (Product # 25887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Chk1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Chk1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

25887-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Chk1 Fusion Protein Ag22993 (262-476 aa encoded by BC004202)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DRWYNKPLK KGAKRPRVTS GGVSESPSGF SKHIQSNLDF SPVNSASSEE NVKYSSSQPE PRTGLSLWDT SPSYIDKLVQ GISFSQPTCP DHMLLNSQLL GTPGSSQNPW QRLVKRMTRF FTKLDADKSY QCLKETCEKL GYQWKKSCMN QVTISTTDRR NNKLIFKVNL LEMDDKILVD FRLSKGDGLE FKRHFLKIKG KLIDIVSSQK VWLPAT (262-476 aa encoded by BC004202)

Target Information

CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability.

Nature communications

Luo Z,Xin D,Liao Y,Berry K,Ogurek S,Zhang F,Zhang L,Zhao C,Rao R,Dong X,Li H,Yu J,Lin Y,Huang G,Xu L,Xin M,Nishinakamura R,Yu J,Kool M,Pfister SM,Roussel MF,Zhou W,Weiss WA,Andreassen P,Lu QR

Fri Feb 10 00:00:00 EST 2023

C11orf54 promotes DNA repair via blocking CMA-mediated degradation of HIF1A.

Communications biology

Tan J,Wang W,Liu X,Xu J,Che Y,Liu Y,Hu J,Hu L,Li J,Zhou Q

Mon Jun 05 00:00:00 EDT 2023

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Cell biology and toxicology

Luo Q,Pan Y,Fu Q,Zhang X,Zhou S,Yu P,Tian H,Liu P,Chen S,Zhang H,Qin T

Sun Oct 01 00:00:00 EDT 2023

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Cell biology and toxicology

Luo Q,Pan Y,Fu Q,Zhang X,Zhou S,Yu P,Tian H,Liu P,Chen S,Zhang H,Qin T

Sun Oct 01 00:00:00 EDT 2023

Inhibition of Checkpoint Kinase 1 (CHK1) Upregulates Interferon Regulatory Factor 1 (IRF1) to Promote Apoptosis and Activate Anti-Tumor Immunity via MICA in Hepatocellular Carcinoma (HCC).

Cancers

Li X,Huang J,Wu Q,Du Q,Wang Y,Huang Y,Cai X,Geller DA,Yan Y

Mon Jan 30 00:00:00 EST 2023

Inhibition of Checkpoint Kinase 1 (CHK1) Upregulates Interferon Regulatory Factor 1 (IRF1) to Promote Apoptosis and Activate Anti-Tumor Immunity via MICA in Hepatocellular Carcinoma (HCC).

Cancers

Li X,Huang J,Wu Q,Du Q,Wang Y,Huang Y,Cai X,Geller DA,Yan Y

Mon Jan 30 00:00:00 EST 2023

Inhibition of Checkpoint Kinase 1 (CHK1) Upregulates Interferon Regulatory Factor 1 (IRF1) to Promote Apoptosis and Activate Anti-Tumor Immunity via MICA in Hepatocellular Carcinoma (HCC).

Cancers

Li X,Huang J,Wu Q,Du Q,Wang Y,Huang Y,Cai X,Geller DA,Yan Y

Mon Jan 30 00:00:00 EST 2023

Nanoengineered Gallium Ion Incorporated Formulation for Safe and Efficient Reversal of PARP Inhibition and Platinum Resistance in Ovarian Cancer.

Research (Washington, D.C.)

Li Y,Cen Y,Tu M,Xiang Z,Tang S,Lu W,Zhang H,Xu J

Mon Mar 20 00:00:00 EDT 2023

Combined Inactivation of CTPS1 and ATR Is Synthetically Lethal to MYC-Overexpressing Cancer Cells.

Cancer research

Sun Z,Zhang Z,Wang QQ,Liu JL

Tue Mar 15 00:00:00 EDT 2022

Deubiquitinase OTUD6A promotes breast cancer progression by increasing TopBP1 stability and rendering tumor cells resistant to DNA-damaging therapy.

Cell death and differentiation

Zhao Y,Huang X,Zhu D,Wei M,Luo J,Yu S,Tian Y,Zheng X

Thu Dec 01 00:00:00 EST 2022

ACTL6A deficiency induces apoptosis through impairing DNA replication and inhibiting the ATR-Chk1 signaling in glioblastoma cells.

Biochemical and biophysical research communications

Hu X,Pei D,Ci M,Zhang G,Li B,Wang J,Shen Y,Zhai X,Liang P,Cui H

Sat Apr 09 00:00:00 EDT 2022

G-quadruplex DNA structures in human stem cells and differentiation.

Nature communications

Zyner KG,Simeone A,Flynn SM,Doyle C,Marsico G,Adhikari S,Portella G,Tannahill D,Balasubramanian S

Mon Jan 10 00:00:00 EST 2022

Inhibition of STAT3^Y705 phosphorylation by Stattic suppresses proliferation and induces mitochondrial-dependent apoptosis in pancreatic cancer cells.

Cell death discovery

Guo H,Xiao Y,Yuan Z,Yang X,Chen J,Chen C,Wang M,Xie L,Chen Q,Tong Y,Zhang Q,Bai Y

Mon Mar 14 00:00:00 EDT 2022

Induction of Mitosis Delay and Apoptosis by CDDO-TFEA in Glioblastoma Multiforme.

Frontiers in pharmacology

Tsai TH,Lieu AS,Huang TY,Kwan AL,Lin CL,Hsu YC

Sat Dec 04 00:00:00 EST 2021

The HDAC and PI3K dual inhibitor CUDC-907 synergistically enhances the antileukemic activity of venetoclax in preclinical models of acute myeloid leukemia.

Haematologica

Li X,Su Y,Hege K,Madlambayan G,Edwards H,Knight T,Polin L,Kushner J,Dzinic SH,White K,Yang J,Miller R,Wang G,Zhao L,Wang Y,Lin H,Taub JW,Ge Y

Sat May 01 00:00:00 EDT 2021

MAP4K1 functions as a tumor promotor and drug mediator for AML via modulation of DNA damage/repair system and MAPK pathway.

EBioMedicine

Ling Q,Li F,Zhang X,Mao S,Lin X,Pan J,Ye W,Wei W,Qian Y,Hu C,Huang X,Wang J,Wang H,Huang J,Wang Y,Jin J

Thu Jul 01 00:00:00 EDT 2021

Azidothymidine (AZT) Inhibits Proliferation of Human Ovarian Cancer Cells by Regulating Cell Cycle Progression.

Anticancer research

Hsieh Y,Tseng JJ

Thu Oct 01 00:00:00 EDT 2020

UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.

Journal of experimental & clinical cancer research : CR

Sun J,Zhu Z,Li W,Shen M,Cao C,Sun Q,Guo Z,Liu L,Wu D

Wed Oct 21 00:00:00 EDT 2020

UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.

Journal of experimental & clinical cancer research : CR

Sun J,Zhu Z,Li W,Shen M,Cao C,Sun Q,Guo Z,Liu L,Wu D

Wed Oct 21 00:00:00 EDT 2020

A novel alkylating deacetylase inhibitor molecule EDO-S101 in combination with cytarabine synergistically enhances apoptosis of acute myeloid leukemia cells.

Medical oncology (Northwood, London, England)

Jin J,Mao S,Li F,Li X,Huang X,Yu M,Guo W,Jin J

Thu Aug 01 00:00:00 EDT 2019

RNF126 as a Biomarker of a Poor Prognosis in Invasive Breast Cancer and CHEK1 Inhibitor Efficacy in Breast Cancer Cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Yang X,Pan Y,Qiu Z,Du Z,Zhang Y,Fa P,Gorityala S,Ma S,Li S,Chen C,Wang H,Xu Y,Yan C,Ruth K,Ma Z,Zhang J

Sun Apr 01 00:00:00 EDT 2018

RNF126 as a Biomarker of a Poor Prognosis in Invasive Breast Cancer and CHEK1 Inhibitor Efficacy in Breast Cancer Cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Yang X,Pan Y,Qiu Z,Du Z,Zhang Y,Fa P,Gorityala S,Ma S,Li S,Chen C,Wang H,Xu Y,Yan C,Ruth K,Ma Z,Zhang J

Sun Apr 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Cell cycle checkpoint kinase; Checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; Serine/threonine-protein kinase Chk1

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Gene Aliases: CHEK1; CHK1

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UniProt ID: (Human) O14757

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Entrez Gene ID: (Human) 1111

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity histone kinase activity (H3-T11 specific) non-receptor serine/threonine protein kinase

Process(es)

DNA damage checkpoint G2/M transition of mitotic cell cycle DNA replication DNA repair cellular response to DNA damage stimulus DNA damage induced protein phosphorylation regulation of double-strand break repair via homologous recombination regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage peptidyl-threonine phosphorylation G2 DNA damage checkpoint histone H3-T11 phosphorylation regulation of cell proliferation negative regulation of mitotic nuclear division regulation of mitotic centrosome separation chromatin-mediated maintenance of transcription cellular response to mechanical stimulus replicative senescence regulation of signal transduction by p53 class mediator regulation of histone H3-K9 acetylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Chk1 Polyclonal Antibody

Chk1 Antibody (25887-1-AP) in ICC/IF

Product Details

25887-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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