Proteintech

Chk1 Monoclonal Antibody (3A3A5)

Chk1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Chk1 Antibody (60277-1-IG) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 60277-1-IG) (Chk1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

60277-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3A3A5

Immunogen

Chk1 Fusion Protein Ag0409 (42-256 aa encoded by BC004202)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MKRAVDCPE NIKKEICINK MLNHENVVKF YGHRREGNIQ YLFLEYCSGG ELFDRIEPDI GMPEPDAQRF FHQLMAGVVY LHGIGITHRD IKPENLLLDE RDNLKISDFG LATVFRYNNR ERLLNKMCGT LPYVAPELLK RREFHAEPVD VWSCGIVLTA MLAGELPWDQ PSDSCQEYSD WKEKKTYLNP WKKIDSAPLA LLHKILVENP SARITI (42-256 aa encoded by BC004202)

Target Information

CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The FBXO32/ATR/ATM axis acts as a molecular switch to control the sensitivity of osteosarcoma cells to irradiation through its regulation of EXO1 expression.

Acta biochimica et biophysica Sinica

Lu Y,Huang P,Li Y,Liu W,Li J,Zhao R,Feng H,Shi C,Cao G

Wed May 24 00:00:00 EDT 2023

Comparable radiation sensitivity in p53 wild-type and p53 deficient tumor cells associated with different cell death modalities.

Cell death discovery

Li P,Liu X,Zhao T,Li F,Wang Q,Zhang P,Hirayama R,Chen W,Jin X,Zheng X,Wang Z,Li Q

Tue Jul 20 00:00:00 EDT 2021

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Nature structural & molecular biology

Zuo X,Zhou J,Li Y,Wu K,Chen Z,Luo Z,Zhang X,Liang Y,Esteban MA,Zhou Y,Fu XD

Mon Feb 01 00:00:00 EST 2021

Bioinformatics

Protein Aliases: Cell cycle checkpoint kinase; checkpoint kinase 1 homolog; Checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; rad27 homolog; Serine/threonine-protein kinase Chk1

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Gene Aliases: C85740; CHEK1; CHK1; rad27

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UniProt ID: (Human) O14757, (Rat) Q91ZN7, (Mouse) O35280

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Entrez Gene ID: (Human) 1111, (Rat) 140583, (Mouse) 12649

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity histone kinase activity (H3-T11 specific) nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups glycosyltransferase non-receptor serine/threonine protein kinase

Process(es)

DNA damage checkpoint G2/M transition of mitotic cell cycle DNA replication DNA repair cellular response to DNA damage stimulus DNA damage induced protein phosphorylation regulation of double-strand break repair via homologous recombination regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage peptidyl-threonine phosphorylation G2 DNA damage checkpoint histone H3-T11 phosphorylation regulation of cell proliferation negative regulation of mitotic nuclear division regulation of mitotic centrosome separation chromatin-mediated maintenance of transcription cellular response to mechanical stimulus replicative senescence regulation of signal transduction by p53 class mediator regulation of histone H3-K9 acetylation protein phosphorylation regulation of gene expression negative regulation of G2/M transition of mitotic cell cycle cellular response to organic substance cellular response to caffeine negative regulation of DNA biosynthetic process cell cycle phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Chk1 Monoclonal Antibody (3A3A5)

Chk1 Antibody (60277-1-IG) in IHC (P)

Product Details

60277-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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