Invitrogen

Chk1 Monoclonal Antibody (DCS-310)

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

5 Published Figures

3 References

View all (73) Chk1 antibodies

Datasheet

Protocols

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Datasheet

Protocols

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FIGURE: 1 / 8

Chk1 Antibody (MA1-91087) in WB

Western blot was performed using anti-Chk1 Mouse Monoclonal Antibody (Product # MA1-91087) a 54 kDa band was observed corresponding to Chk1 across the cell lines tested along with uncharacterized bands (*). Whole cell extracts of (30 µg lysate) of HeLa (Lane 1), SHSY5Y (Lane 2), HCT 116 (Lane 3), A549 (Lane 4), K562 (Lane 5) and HEK 293 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blott... View More

Chk1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Chk1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Chk1 Antibody — FIGURE 6. Che-1 binds pericentrin and mediates its binding to Chk1. A, human lymphoblastoid AHH1 cells and CV1576 lymphoblastoid cells lacking PCNT expression were immunostained with the indicated antibodies. Bar diagrams show the percentages of cells with centrosomal colocalization of γ-tubulin and Che-1. B, Western blot analysis of isolated centrosome preparations or whole cell lysates from human lymphoblastoid AHH1 cells and PCNT-deficient cells. C, Western blot analysis of HCT116 cells untreated or treated with 1 µm Doxorubicin for 2 h. Total cellular extracts were immunoprecipitated (I.P.) with anti-Che-1 antibody and analyzed by Western blot using the indicated antibodies. D, total cellular extracts from HCT116 cells transiently transfected with GFP siRNA (siControl) or Che-1 siRNA (siChe-1) were immunoprecipitated with anti-Chk1 antibody and analyzed by Western blot using the indicated antibodies. The scale bar represents 5 µm. E, total cellular extracts from human lymphoblastoid AHH1 cells and CV1576 PCNT-deficient cells were immunoprecipitated (I.P.) with anti-Che-1 antibody and analyzed by Western blot using the indicated antibodies. F, total cellular extracts from HCT116 cells transiently transfected with GFP siRNA (siControl) or Chk1 siRNA (siChk1) were immunoprecipitated (I.P.) with anti-Che-1 antibody and analyzed by Western blot using the indicated antibodies. Immunoprecipitation negative control (Neg. control) was performed by using normal rabbit IgG. Scale bar represents 5 µm. Error bars represent S.D. after combining the results of {KD}

Product Details

MA1-91087

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

Immunoprecipitation (IP)

2 µg / 0.2 mL of cell extract

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

DCS-310

Immunogen

Recombinant full length protein (Human).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2080477

Product Specific Information

Heat in a microwave oven, twice for 10 minutes in citrate buffer, pH 6.5.

Target Information

CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chk1 Inhibition of the Replication Factor Drf1 Guarantees Cell-Cycle Elongation at the Xenopus laevis Mid-blastula Transition.

Developmental cell

Collart C,Smith JC,Zegerman P

Published figure using Chk1 monoclonal antibody (Product # MA1-91087) in Western Blot

Mon Jul 10 00:00:00 EDT 2017

Centrosomal Che-1 protein is involved in the regulation of mitosis and DNA damage response by mediating pericentrin (PCNT)-dependent Chk1 protein localization.

The Journal of biological chemistry

Sorino C,Bruno T,Desantis A,Di Certo MG,Iezzi S,De Nicola F,Catena V,Floridi A,Chessa L,Passananti C,Cundari E,Fanciulli M

MA1-91087 was used in western blot the study the mechanism by which centrosomal Che-1 regulates the DNA damage response and mitosis

Fri Aug 09 00:00:00 EDT 2013

Centrosomal Che-1 protein is involved in the regulation of mitosis and DNA damage response by mediating pericentrin (PCNT)-dependent Chk1 protein localization.

The Journal of biological chemistry

Sorino C,Bruno T,Desantis A,Di Certo MG,Iezzi S,De Nicola F,Catena V,Floridi A,Chessa L,Passananti C,Cundari E,Fanciulli M

MA1-91087 was used in western blot the study the mechanism by which centrosomal Che-1 regulates the DNA damage response and mitosis

Fri Aug 09 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Cell cycle checkpoint kinase; checkpoint kinase 1 homolog; Checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; rad27 homolog; Serine/threonine-protein kinase Chk1

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Gene Aliases: C85740; CHEK1; CHK1; rad27

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UniProt ID: (Human) O14757, (Rat) Q91ZN7, (Mouse) O35280

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Entrez Gene ID: (Human) 1111, (Rat) 140583, (Mouse) 12649

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity histone kinase activity (H3-T11 specific) nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups glycosyltransferase non-receptor serine/threonine protein kinase

Process(es)

DNA damage checkpoint G2/M transition of mitotic cell cycle DNA replication DNA repair cellular response to DNA damage stimulus DNA damage induced protein phosphorylation regulation of double-strand break repair via homologous recombination regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage peptidyl-threonine phosphorylation G2 DNA damage checkpoint histone H3-T11 phosphorylation regulation of cell proliferation negative regulation of mitotic nuclear division regulation of mitotic centrosome separation chromatin-mediated maintenance of transcription cellular response to mechanical stimulus replicative senescence regulation of signal transduction by p53 class mediator regulation of histone H3-K9 acetylation protein phosphorylation regulation of gene expression negative regulation of G2/M transition of mitotic cell cycle cellular response to organic substance cellular response to caffeine negative regulation of DNA biosynthetic process cell cycle phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Chk1 Monoclonal Antibody (DCS-310)

Chk1 Antibody (MA1-91087) in WB

Product Details

MA1-91087

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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