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Invitrogen
Clathrin Heavy Chain Recombinant Rabbit Monoclonal Antibody (JA53-02)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
Clathrin Heavy Chain Antibody (MA5-32690) in ICC/IF
Immunofluorescence analysis of CLTC/CLTCL1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Clathrin Heavy Chain Recombinant Rabbit Monoclonal Antibody (JA53-02) (Product # MA5-32690) at 1:100 in 0.1% BS... View More
Product Details
MA5-32690
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Non-human primate,
Rat,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JA53-02
Immunogen
Synthetic peptide within Human Clathrin heavy chain aa 201-250.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809967
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain on chromosome 17; Clathrin heavy chain on chromosome 22; clathrin, heavy chain (Hc); clathrin, heavy chain-like 1; clathrin, heavy polypeptide (Hc); Clathrin, heavy polypeptide D; clathrin, heavy polypeptide-like 1; clathrin, heavy polypeptide-like 2; CLH-17; CLH-22
Gene Aliases: 3110065L21Rik; CHC; CHC17; CHC22; CLH-17; CLH17; CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD; Hc; KIAA0034; R74732
UniProt ID: (Human) Q00610, (Human) P53675, (Rat) P11442, (Mouse) Q68FD5
Entrez Gene ID: (Human) 1213, (Human) 8218, (Rat) 54241, (Mouse) 67300
osteoblast differentiation
intracellular protein transport
receptor-mediated endocytosis
mitotic nuclear division
antigen processing and presentation of exogenous peptide antigen via MHC class II
receptor internalization
transferrin transport
retrograde transport, endosome to Golgi
Wnt signaling pathway, planar cell polarity pathway
negative regulation of hyaluronan biosynthetic process
negative regulation of protein localization to plasma membrane
signal transduction
anatomical structure morphogenesis
positive regulation of glucose import
Golgi organization
vesicle-mediated transport
mitotic spindle assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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