Invitrogen

Clathrin Heavy Chain Monoclonal Antibody (X22), Alexa Fluor™ 488

10 Published Figures

9 References

View all (36) Clathrin Heavy Chain antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (10)

Clathrin Heavy Chain Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

Clathrin Heavy Chain Antibody (MA1-065-A488) in ICC/IF

Immunofluorescent analysis of Clathrin Heavy Chain (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Clathrin Heavy Chain Monoclonal Antibody, AlexaFluor 488 conjugate (Product # MA1-065-A488) at a dilution of 5 µg/mL in blocking buffer ... View More

Clathrin Heavy Chain Antibody in Immunocytochemistry, Immunoprecipitation (ICC/IF, IP)

Clathrin Heavy Chain Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

Clathrin Heavy Chain Antibody in Western Blot (WB)

Clathrin Heavy Chain Antibody in Immunoprecipitation (IP)

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Product Details

MA1-065-A488

Applications

Tested Dilution

Publications

Western Blot (WB)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1-10 µg/mL

View 3 publications 3 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

X22

Immunogen

Purified human brain clathrin heavy chain

View immunogen

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2662916

Product Specific Information

MA1065A488 detects clathrin heavy chain in non-human primate, bovine, human, rat and mouse tissues as well as hamster (CHO) cells.

The MA1065A488 immunogen is purified human brain clathrin heavy chain. Electron microscopy and proteolysis mapping demonstrate that MA1065A488 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.

Target Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients.

Frontiers in molecular neuroscience

Poulsen ET,Iannuzzi F,Rasmussen HF,Maier TJ,Enghild JJ,Jørgensen AL,Matrone C

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Immunoprecipitation

Mon Sep 25 00:00:00 EDT 2023

An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients.

Frontiers in molecular neuroscience

Poulsen ET,Iannuzzi F,Rasmussen HF,Maier TJ,Enghild JJ,Jørgensen AL,Matrone C

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Immunoprecipitation

Mon Sep 25 00:00:00 EDT 2023

ROCK1 is a novel Rac1 effector to regulate tubular endocytic membrane formation during clathrin-independent endocytosis.

Scientific reports

Soriano-Castell D,Chavero A,Rentero C,Bosch M,Vidal-Quadras M,Pol A,Enrich C,Tebar F

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Western Blot

Mon Jul 31 00:00:00 EDT 2017

Surface Toll-like receptor 3 expression in metastatic intestinal epithelial cells induces inflammatory cytokine production and promotes invasiveness.

The Journal of biological chemistry

Bugge M,Bergstrom B,Eide OK,Solli H,Kjønstad IF,Stenvik J,Espevik T,Nilsen NJ

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Western Blot

Fri Sep 15 00:00:00 EDT 2017

Endocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial Cells.

PloS one

Chiang CF,Flint M,Lin JS,Spiropoulou CF

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Western Blot

Tue Jun 13 00:00:00 EDT 2017

Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance.

Nature neuroscience

Zhao Z,Sagare AP,Ma Q,Halliday MR,Kong P,Kisler K,Winkler EA,Ramanathan A,Kanekiyo T,Bu G,Owens NC,Rege SV,Si G,Ahuja A,Zhu D,Miller CA,Schneider JA,Maeda M,Maeda T,Sugawara T,Ichida JK,Zlokovic BV

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Immunofluorescence

Wed Jul 01 00:00:00 EDT 2015

Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance.

Nature neuroscience

Zhao Z,Sagare AP,Ma Q,Halliday MR,Kong P,Kisler K,Winkler EA,Ramanathan A,Kanekiyo T,Bu G,Owens NC,Rege SV,Si G,Ahuja A,Zhu D,Miller CA,Schneider JA,Maeda M,Maeda T,Sugawara T,Ichida JK,Zlokovic BV

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Immunofluorescence

Wed Jul 01 00:00:00 EDT 2015

Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance.

Nature neuroscience

Zhao Z,Sagare AP,Ma Q,Halliday MR,Kong P,Kisler K,Winkler EA,Ramanathan A,Kanekiyo T,Bu G,Owens NC,Rege SV,Si G,Ahuja A,Zhu D,Miller CA,Schneider JA,Maeda M,Maeda T,Sugawara T,Ichida JK,Zlokovic BV

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Immunofluorescence

Wed Jul 01 00:00:00 EDT 2015

The X-linked mental retardation protein OPHN1 interacts with Homer1b/c to control spine endocytic zone positioning and expression of synaptic potentiation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Nakano-Kobayashi A,Tai Y,Nadif Kasri N,Van Aelst L

Published figure using Clathrin Heavy Chain monoclonal antibody (Product # MA1-065-A488) in Immunofluorescence

Wed Jun 25 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain on chromosome 17; Clathrin heavy chain on chromosome 22; clathrin, heavy chain (Hc); clathrin, heavy chain-like 1; clathrin, heavy polypeptide (Hc); Clathrin, heavy polypeptide D; clathrin, heavy polypeptide-like 1; clathrin, heavy polypeptide-like 2; CLH-17; CLH-22

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Gene Aliases: 3110065L21Rik; CHC; CHC17; CHC22; CLH-17; CLH17; CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD; Hc; KIAA0034; R74732

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UniProt ID: (Human) Q00610, (Bovine) P49951, (Human) P53675, (Rat) P11442, (Mouse) Q68FD5

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Entrez Gene ID: (Human) 1213, (Bovine) 281080, (Human) 8218, (Chinese hamster) 100756644, (Rat) 54241, (Mouse) 67300

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Function(s)

double-stranded RNA binding structural molecule activity protein binding protein kinase binding clathrin light chain binding poly(A) RNA binding protein domain specific binding identical protein binding disordered domain specific binding arrestin family protein binding signal transducer activity protein C-terminus binding ankyrin binding heat shock protein binding peptide binding vesicle coat protein

Process(es)

osteoblast differentiation intracellular protein transport receptor-mediated endocytosis mitotic nuclear division antigen processing and presentation of exogenous peptide antigen via MHC class II receptor internalization transferrin transport retrograde transport, endosome to Golgi Wnt signaling pathway, planar cell polarity pathway negative regulation of hyaluronan biosynthetic process negative regulation of protein localization to plasma membrane autophagy clathrin coat assembly cell division signal transduction anatomical structure morphogenesis positive regulation of glucose import Golgi organization vesicle-mediated transport mitotic spindle assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Clathrin Heavy Chain Monoclonal Antibody (X22), Alexa Fluor™ 488

Clathrin Heavy Chain Antibody (MA1-065-A488) in ICC/IF

Product Details

MA1-065-A488

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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