Invitrogen

Claudin 18 Polyclonal Antibody

Claudin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Claudin 18 Antibody (38-8000) in IHC (P)

Immunohistochemistry analysis of Claudin-18 (C-Term) showing staining in the membrane of paraffin-embedded human stomach tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room t... View More

Claudin 18 Antibody in Western Blot (WB)

Claudin 18 Antibody in Immunohistochemistry (IHC)

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Product Details

38-8000

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the human Claudin-18 protein, which differs from mouse by 4 conservative amino acid replacements and one non-conservative amino acid replacement

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533382

Target Information

Claudin-18 is a tight junction protein expressed as lung-and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene. The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor. A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human. Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein. Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells. These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach. Claudin-18 expression has also been reported in the inner ear.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Asymmetric distribution of dynamin-2 and β-catenin relative to tight junction spikes in alveolar epithelial cells.

Tissue barriers

Lynn KS,Easley KF,Martinez FJ,Reed RC,Schlingmann B,Koval M

38-8000 was used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry to suggest a novel role for dynamin-2 in tight junction spike formation by reorienting junction-associated actin.

Sat Jul 03 00:00:00 EDT 2021

Clinicopathological significance of intelectin-1 in colorectal cancer: Intelectin-1 participates in tumor suppression and favorable progress.

Pathology international

Katsuya N,Sentani K,Sekino Y,Yamamoto Y,Kobayashi G,Babasaki T,Oue N,Amatya VJ,Takeshima Y,Yasui W

38-8000 was used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to indicate that ITLN1 plays an important role in regulating colorectal tumorigenesis, tending toward a more favorable prognosis.

Tue Dec 01 00:00:00 EST 2020

Mucin 5B, carbonic anhydrase 9 and claudin 18 are potential theranostic markers of gallbladder carcinoma.

Histopathology

Espinoza JA,Riquelme I,Sagredo EA,Rosa L,García P,Bizama C,Apud-Bell M,Leal P,Weber H,Benavente F,Vargas S,Romero D,Kalergis AM,Roa JC

38-8000 was used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to find that mRNA and protein levels of mucin 5B (MUC5B) and carbonic anhydrase 9 (CA9) were highly increased in gallbladder tissues.

Fri Mar 01 00:00:00 EST 2019

The Cdx2 homeobox gene suppresses intestinal tumorigenesis through non-cell-autonomous mechanisms.

The Journal of experimental medicine

Balbinot C,Armant O,Elarouci N,Marisa L,Martin E,De Clara E,Onea A,Deschamps J,Beck F,Freund JN,Duluc I

Published figure using Claudin 18 polyclonal antibody (Product # 38-8000) in Immunohistochemistry

Mon Mar 05 00:00:00 EST 2018

The Cdx2 homeobox gene suppresses intestinal tumorigenesis through non-cell-autonomous mechanisms.

The Journal of experimental medicine

Balbinot C,Armant O,Elarouci N,Marisa L,Martin E,De Clara E,Onea A,Deschamps J,Beck F,Freund JN,Duluc I

Published figure using Claudin 18 polyclonal antibody (Product # 38-8000) in Immunohistochemistry

Mon Mar 05 00:00:00 EST 2018

Frequent CLDN18-ARHGAP fusion in highly metastatic diffuse-type gastric cancer with relatively early onset.

Oncotarget

Tanaka A,Ishikawa S,Ushiku T,Yamazawa S,Katoh H,Hayashi A,Kunita A,Fukayama M

38-8000 was used in Immunohistochemistry to examine the early-onset CLDN18-ARHGAP fusion-positive diffuse-type gastric cancer as a biologically distinct entity requiring more advanced therapeutic options.

Fri Jun 29 00:00:00 EDT 2018

Receptor for advanced glycation endproducts (RAGE) maintains pulmonary structure and regulates the response to cigarette smoke.

PloS one

Wolf L,Herr C,Niederstraßer J,Beisswenger C,Bals R

38-8000 was used in Western Blotting to suggest that RAGE regulates the differentiation of alveolar epithelial cells and impacts on the development and maintenance of pulmonary structure.

Fri Sep 22 00:00:00 EDT 2017

Receptor for advanced glycation endproducts (RAGE) maintains pulmonary structure and regulates the response to cigarette smoke.

PloS one

Wolf L,Herr C,Niederstraßer J,Beisswenger C,Bals R

38-8000 was used in Western Blotting to suggest that RAGE regulates the differentiation of alveolar epithelial cells and impacts on the development and maintenance of pulmonary structure.

Fri Sep 22 00:00:00 EDT 2017

P2X7R-dependent regulation of glycogen synthase kinase 3β and claudin-18 in alveolar epithelial type I cells of mice lung.

Histochemistry and cell biology

Barth K,Bläsche R,Neißer A,Bramke S,Frank JA,Kasper M

388000 was used in immunohistochemistry - paraffin section and immunoprecipitation to elucidate the role of P2X7 in alveolar epithelial cells

Thu Dec 01 00:00:00 EST 2016

Inhibition of BMP activity protects epithelial barrier function in lung injury.

The Journal of pathology

Helbing T,Herold EM,Hornstein A,Wintrich S,Heinke J,Grundmann S,Patterson C,Bode C,Moser M

38-8000 was used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to investigate the role of BMP2 and BMPER signaling and mechanisms involved in injured lung epithelium.

Sun Sep 01 00:00:00 EDT 2013

Claudin 18 is a novel negative regulator of bone resorption and osteoclast differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Linares GR,Brommage R,Powell DR,Xing W,Chen ST,Alshbool FZ,Lau KH,Wergedal JE,Mohan S

Sun Jul 01 00:00:00 EDT 2012

Expression of claudins 7 and 18 in pancreatic ductal adenocarcinoma: association with features of differentiation.

Journal of clinical pathology

Soini Y,Takasawa A,Eskelinen M,Juvonen P,Kärjä V,Hasegawa T,Murata M,Tanaka S,Kojima T,Sawada N

38-8000 was used in immunohistochemistry - paraffin section to measure the expression of claudins 7 and 8 in pancreatic ductal adenocarcinoma.

Tue May 01 00:00:00 EDT 2012

Claudin-18 is an early-stage marker of pancreatic carcinogenesis.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Tanaka M,Shibahara J,Fukushima N,Shinozaki A,Umeda M,Ishikawa S,Kokudo N,Fukayama M

Sat Oct 01 00:00:00 EDT 2011

Claudins 10 and 18 are predominantly expressed in lung adenocarcinomas and in tumors of nonsmokers.

International journal of clinical and experimental pathology

Merikallio H,Pääkkö P,Harju T,Soini Y

38-8000 was used in immunohistochemistry to measure claudins 8 and 0 expression in primary lung carcinomas.

Fri Mar 02 00:00:00 EST 2012

Bioinformatics

Protein Aliases: Claudin-18; surfactant associated 5; surfactant associated protein J; surfactant, pulmonary associated protein J

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Gene Aliases: CLDN18; SFTA5; SFTPJ; UNQ778/PRO1572

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UniProt ID: (Human) P56856, (Mouse) P56857

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Entrez Gene ID: (Human) 51208, (Mouse) 56492

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Function(s)

structural molecule activity identical protein binding tight junction

Process(es)

calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules negative regulation of bone resorption digestive tract development TNFSF11-mediated signaling pathway negative regulation of protein localization to nucleus negative regulation of osteoclast development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Claudin 18 Polyclonal Antibody

Claudin 18 Antibody (38-8000) in IHC (P)

Product Details

38-8000

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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