Invitrogen

Claudin 18 Recombinant Rabbit Monoclonal Antibody (34H14L15)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

20 Published Figures

27 References

View all (9) Claudin 18 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

Claudin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Claudin 18 Antibody (700178) in IHC (P)

Immunohistochemistry analysis of Claudin-18 was performed on paraffin-embedded human normal stomach and squamous lung carcinoma tissue sections. To expose target proteins, endogenous peroxide blocking followed by heat induced epitope retrieval was performed using Tris-EDTA (pH 9. 0) buffer for 15 minutes. Following antigen retrieval, tissues were blocked in 0. 2% BSA with 0. 1% cold fish skin gelatin in 1X ... View More

Claudin 18 Antibody in Immunocytochemistry (ICC/IF)

Claudin 18 Antibody in Western Blot (WB)

Claudin 18 Antibody in Immunohistochemistry (IHC)

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Product Details

700178

Applications

Tested Dilution

Publications

Western Blot (WB)

2-3 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 6 publications 6 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Amphibian, Dog, Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

34H14L15

Immunogen

A peptide corresponding to amino acids 224-237 of P56856.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532290

Product Specific Information

This antibody is predicted to react with chimpanzee, equine, rat and Rhesus monkey based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.The immunogenic sequence for this antibody is GFKASTGFGSNTKNH, and the antibody is predicted to react with chimpanzee, equine, rat, and Rhesus monkey based on sequence identity and similarity.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Claudin-18 is a tight junction protein expressed as lung-and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene. The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor. A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human. Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein. Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells. These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach. Claudin-18 expression has also been reported in the inner ear.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Functional Analysis of Gastric Tight Junction Proteins in Xenopus laevis Oocytes.

Membranes

Stein L,Brunner N,Amasheh S

Published figure using Claudin 18 recombinant monoclonal antibody (Product # 700178) in Western Blot

Sat Jul 23 00:00:00 EDT 2022

Functional Analysis of Gastric Tight Junction Proteins in Xenopus laevis Oocytes.

Membranes

Stein L,Brunner N,Amasheh S

Published figure using Claudin 18 recombinant monoclonal antibody (Product # 700178) in Western Blot

Sat Jul 23 00:00:00 EDT 2022

Functional Analysis of Gastric Tight Junction Proteins in Xenopus laevis Oocytes.

Membranes

Stein L,Brunner N,Amasheh S

Published figure using Claudin 18 recombinant monoclonal antibody (Product # 700178) in Western Blot

Sat Jul 23 00:00:00 EDT 2022

Transcriptomic Analysis of Testicular Gene Expression in a Dog Model of Experimentally Induced Cryptorchidism.

Cells

Jhun H,Lee WY,Park JK,Hwang SG,Park HJ

700178 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to indicate that testicular hyperthermia caused by cryptorchidism affects claudin expression, regulated germ cell death, and the proliferation of Sertoli cells.

Wed Aug 10 00:00:00 EDT 2022

Transcriptomic Analysis of Testicular Gene Expression in a Dog Model of Experimentally Induced Cryptorchidism.

Cells

Jhun H,Lee WY,Park JK,Hwang SG,Park HJ

Wed Aug 10 00:00:00 EDT 2022

Asymmetric distribution of dynamin-2 and β-catenin relative to tight junction spikes in alveolar epithelial cells.

Tissue barriers

Lynn KS,Easley KF,Martinez FJ,Reed RC,Schlingmann B,Koval M

700178 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest a novel role for dynamin-2 in tight junction spike formation by reorienting junction-associated actin.

Sat Jul 03 00:00:00 EDT 2021

Clinical Significance of CLDN18.2 Expression in Metastatic Diffuse-Type Gastric Cancer.

Journal of gastric cancer

Kim SR,Shin K,Park JM,Lee HH,Song KY,Lee SH,Kim B,Kim SY,Seo J,Kim JO,Roh SY,Kim IH

700178 was used in Immunohistochemistry-immunofluorescence to investigate the clinical significance of CLDN18.2 and other cell-adherens junction molecules (Rho GTPase-activating protein [RhoGAP] and E-cadherin) in metastatic diffuse-type gastric cancer (mDGC).

Tue Dec 01 00:00:00 EST 2020

Clinical Significance of CLDN18.2 Expression in Metastatic Diffuse-Type Gastric Cancer.

Journal of gastric cancer

Kim SR,Shin K,Park JM,Lee HH,Song KY,Lee SH,Kim B,Kim SY,Seo J,Kim JO,Roh SY,Kim IH

Tue Dec 01 00:00:00 EST 2020

Core Binding Factors are essential for ovulation, luteinization, and female fertility in mice.

Scientific reports

Lee-Thacker S,Jeon H,Choi Y,Taniuchi I,Takarada T,Yoneda Y,Ko C,Jo M

700178 was used in Immunohistochemistry to provide in vivo evidence that CBFs act as essential transcriptional regulators of both ovulation and luteinization by regulating the expression of key genes that are involved in inflammation, matrix remodeling, cell differentiation, vascularization, and steroid metabolisms in mice.

Thu Jun 18 00:00:00 EDT 2020

Core Binding Factors are essential for ovulation, luteinization, and female fertility in mice.

Scientific reports

Lee-Thacker S,Jeon H,Choi Y,Taniuchi I,Takarada T,Yoneda Y,Ko C,Jo M

Thu Jun 18 00:00:00 EDT 2020

Nuclear staining of claudin-18 is a new immunohistochemical marker for diagnosing intramucosal well-differentiated gastric adenocarcinoma.

Pathology international

Takahashi Y,Tsutsumi Y,Takeuchi C,Shiogama K,Mizutani Y,Inada KI,Yamamichi N,Koike K

700178 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to evaluate the association between histopathology and immunohistochemical expression of MUC1, HER2, p53, CEA, E-cadherin, β-catenin and claudin-18.

Tue Sep 01 00:00:00 EDT 2020

Prevention and Reversion of Pancreatic Tumorigenesis through a Differentiation-Based Mechanism.

Developmental cell

Krah NM,Narayanan SM,Yugawa DE,Straley JA,Wright CVE,MacDonald RJ,Murtaugh LC

700178 was used in Immunohistochemistry to suggest that reactivation of an endogenous differentiation program can prevent and reverse oncogene-driven transformation in cells harboring tumor-driving mutations, introducing a potential paradigm for solid tumor prevention and treatment.

Mon Sep 23 00:00:00 EDT 2019

Pancreatic Ductal Deletion of Hnf1b Disrupts Exocrine Homeostasis, Leads to Pancreatitis, and Facilitates Tumorigenesis.

Cellular and molecular gastroenterology and hepatology

Quilichini E,Fabre M,Dirami T,Stedman A,De Vas M,Ozguc O,Pasek RC,Cereghini S,Morillon L,Guerra C,Couvelard A,Gannon M,Haumaitre C

700178 was used in Immunohistochemistry to reveal that Hnf1b deficiency may contribute to diseases of the exocrine pancreas and could gain further insight into the etiology of pancreatitis and tumorigenesis.

Mon Jul 20 00:00:00 EDT 2020

Lung injury after asphyxia and hemorrhagic shock in newborn piglets: Analysis of structural and inflammatory changes.

PloS one

Weber B,Mendler MR,Lackner I,von Zelewski A,Höfler S,Baur M,Braun CK,Hummler H,Schwarz S,Pressmar J,Kalbitz M

700178 was used in Immunocytochemistry-immunoflourescence to study early morphological, immunological and structural alterations in lung tissue after asphyxia and hemorrhage.

Fri Feb 21 00:00:00 EST 2020

Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis.

The Journal of clinical investigation

Zhou B,Flodby P,Luo J,Castillo DR,Liu Y,Yu FX,McConnell A,Varghese B,Li G,Chimge NO,Sunohara M,Koss MN,Elatre W,Conti P,Liebler JM,Yang C,Marconett CN,Laird-Offringa IA,Minoo P,Guan K,Stripp BR,Crandall ED,Borok Z

700178 was used in Immunocytochemistry to establish CLDN18 as a regulator of YAP activity that serves to restrict organ size, progenitor cell proliferation, and tumorigenesis in the murine lung.

Thu Mar 01 00:00:00 EST 2018

Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis.

The Journal of clinical investigation

700178 was used in Immunocytochemistry to establish CLDN18 as a regulator of YAP activity that serves to restrict organ size, progenitor cell proliferation, and tumorigenesis in the murine lung.

Thu Mar 01 00:00:00 EST 2018

Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis.

The Journal of clinical investigation

700178 was used in Immunocytochemistry to establish CLDN18 as a regulator of YAP activity that serves to restrict organ size, progenitor cell proliferation, and tumorigenesis in the murine lung.

Thu Mar 01 00:00:00 EST 2018

CLDN18.1 attenuates malignancy and related signaling pathways of lung adenocarcinoma in vivo and in vitro.

International journal of cancer

Luo J,Chimge NO,Zhou B,Flodby P,Castaldi A,Firth AL,Liu Y,Wang H,Yang C,Marconett CN,Crandall ED,Offringa IA,Frenkel B,Borok Z

Published figure using Claudin 18 recombinant monoclonal antibody (Product # 700178) in Western Blot

Sat Dec 15 00:00:00 EST 2018

Vitamin D Receptor Deletion Leads to the Destruction of Tight and Adherens Junctions in Lungs.

Tissue barriers

Chen H,Lu R,Zhang YG,Sun J

700178 was used in Western Blotting to study the role of the vitamin D receptor in lung barrier integrity.

Mon Apr 01 00:00:00 EDT 2019

The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma.

eLife

Krah NM,De La O JP,Swift GH,Hoang CQ,Willet SG,Chen Pan F,Cash GM,Bronner MP,Wright CV,MacDonald RJ,Murtaugh LC

Published figure using Claudin 18 monoclonal antibody (Product # 700178) in Immunohistochemistry

Tue Jul 07 00:00:00 EDT 2015

The relative balance of GM-CSF and TGF-β1 regulates lung epithelial barrier function.

American journal of physiology. Lung cellular and molecular physiology

Overgaard CE,Schlingmann B,Dorsainvil White S,Ward C,Fan X,Swarnakar S,Brown LA,Guidot DM,Koval M

700178 was used in western blot to test if the relative balance of GM-CSF and TGF-β signaling regulates lung epithelial barrier function.

Mon Jun 15 00:00:00 EDT 2015

The relative balance of GM-CSF and TGF-β1 regulates lung epithelial barrier function.

American journal of physiology. Lung cellular and molecular physiology

Overgaard CE,Schlingmann B,Dorsainvil White S,Ward C,Fan X,Swarnakar S,Brown LA,Guidot DM,Koval M

700178 was used in western blot to test if the relative balance of GM-CSF and TGF-β signaling regulates lung epithelial barrier function.

Mon Jun 15 00:00:00 EDT 2015

NF-κB inhibitors impair lung epithelial tight junctions in the absence of inflammation.

Tissue barriers

Ward C,Schlingmann B,Stecenko AA,Guidot DM,Koval M

700178 was used in immunohistochemistry to investigate the effect of NF-kappaB inhibitors on lung epithelial tight junctions in the absence of inflammation

Fri Apr 03 00:00:00 EDT 2015

NF-κB inhibitors impair lung epithelial tight junctions in the absence of inflammation.

Tissue barriers

Ward C,Schlingmann B,Stecenko AA,Guidot DM,Koval M

700178 was used in immunohistochemistry to investigate the effect of NF-kappaB inhibitors on lung epithelial tight junctions in the absence of inflammation

Fri Apr 03 00:00:00 EDT 2015

CD4+ T lymphocyte ablation prevents pancreatic carcinogenesis in mice.

Cancer immunology research

Zhang Y,Yan W,Mathew E,Bednar F,Wan S,Collins MA,Evans RA,Welling TH,Vonderheide RH,di Magliano MP

Published figure using Claudin 18 recombinant monoclonal antibody (Product # 700178) in Immunohistochemistry

Thu May 01 00:00:00 EDT 2014

Primary gastric Merkel cell carcinoma harboring DNA polyomavirus: first description of an unusual high-grade neuroendocrine carcinoma.

Human pathology

Capella C,Marando A,Longhi E,Bernasconi B,Finzi G,Parravicini C,Sessa F,La Rosa S

700178 was used in immunohistochemistry to reoprt on a case of an unusual primary gastric Merkel cell carcinoma

Sun Jun 01 00:00:00 EDT 2014

Interleukin-6 is required for pancreatic cancer progression by promoting MAPK signaling activation and oxidative stress resistance.

Cancer research

Zhang Y,Yan W,Collins MA,Bednar F,Rakshit S,Zetter BR,Stanger BZ,Chung I,Rhim AD,di Magliano MP

Published figure using Claudin 18 recombinant monoclonal antibody (Product # 700178) in Immunohistochemistry

Tue Oct 15 00:00:00 EDT 2013

Linear and micronodular neuroendocrine cell hyperplasia in an ovarian mucinous cystadenoma.

Pathology, research and practice

Dainese E,Cimetti L,Capella C,Riva C

700178 was used in immunohistochemistry to report on a case of ovarian mucinous cystadenoma

Tue Oct 01 00:00:00 EDT 2013

Distribution of LGR5+ cells and associated implications during the early stage of gastric tumorigenesis.

PloS one

Jang BG,Lee BL,Kim WH

700178 was used in immunohistochemistry to study the potential cancer stem cell role of LGR5+ cells in gastric tumorigenesis

Fri Sep 19 00:00:00 EDT 2014

Oncogenic Kras is required for both the initiation and maintenance of pancreatic cancer in mice.

The Journal of clinical investigation

Collins MA,Bednar F,Zhang Y,Brisset JC,Galbán S,Galbán CJ,Rakshit S,Flannagan KS,Adsay NV,Pasca di Magliano M

Published figure using Claudin 18 recombinant monoclonal antibody (Product # 700178) in Immunohistochemistry

Wed Feb 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: claudin 18-like; Claudin-18; surfactant associated 5; surfactant associated protein J; surfactant, pulmonary associated protein J

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Gene Aliases: CLDN18; SFTA5; SFTPJ; UNQ778/PRO1572

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UniProt ID: (Human) P56856, (Mouse) P56857, (Rat) Q5I0E5

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Entrez Gene ID: (Human) 51208, (Mouse) 56492, (Rat) 315953

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Function(s)

structural molecule activity identical protein binding tight junction

Process(es)

calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules negative regulation of bone resorption digestive tract development TNFSF11-mediated signaling pathway negative regulation of protein localization to nucleus negative regulation of osteoclast development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Claudin 18 Recombinant Rabbit Monoclonal Antibody (34H14L15)

Claudin 18 Antibody (700178) in IHC (P)

Product Details

700178

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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