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Invitrogen
Claudin 4 Monoclonal Antibody (3E2C1), Alexa Fluor™ 488
FIGURE: 1 / 2
Claudin 4 Antibody (329488) in IHC (P)
Immunohistochemistry analysis of Claudin 4 showing staining in the tight junctions of paraffin-embedded human colon tissue. To expose target proteins, antigen retrieval was performed by heating the tissue to 95 C in 10mM sodium citrate (pH 6.0) for 20 min. Following antigen retrieval, tissues were blocked in 3% H2O2 for 10 min at room temperature, washed with ddH2O and PBS, and then blocked with 10% goat se... View More
Product Details
329488
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Dog,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3E2C1
Immunogen
Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin-4.
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2532183
Target Information
Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Claudin-4; CLAUDIN4; Clostridium perfringens enterotoxin receptor; Clostridium perfringens enterotoxin receptor 1; CPE-R; CPE-receptor; h CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein
Gene Aliases: Cep-r; CLDN4; CPE-R; CPER; CPETR; CPETR1; hCPE-R; WBSCR8
UniProt ID: (Human) O14493, (Dog) A0A8C0N7E5, (Mouse) O35054
Entrez Gene ID: (Human) 1364, (Dog) 100856416, (Rat) 304407, (Mouse) 12740
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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