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Invitrogen
Claudin 5 Recombinant Rabbit Monoclonal Antibody (JM11-22)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 10
Claudin 5 Antibody (MA5-32614) in ICC/IF
Immunofluorescence analysis of CLDN5 was performed using 90% confluent log phase HUVEC cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Claudin 5 Recombinant Rabbit Monoclonal Antibody (JM11-22) (Product # MA5-32614) at 1:100 dilution in 0.1% BSA, ... View More
Product Details
MA5-32614
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JM11-22
Immunogen
Synthetic peptide within Human Claudin 5 aa 191-214
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809891
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Claudin-5; TMDVCF; Transmembrane protein deleted in VCFS; transmembrane protein deleted in velocardiofacial syndrome
Gene Aliases: AWAL; BEC1; CLDN5; CPETRL1; TMVCF
UniProt ID: (Human) O00501
Entrez Gene ID: (Human) 7122
outflow tract morphogenesis
cell-cell junction assembly
transforming growth factor beta receptor signaling pathway
learning
calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules
palate development
face morphogenesis
positive regulation of establishment of endothelial barrier
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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