Invitrogen

Claudin 8 Polyclonal Antibody

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

9 Published Figures

14 References

View all (4) Claudin 8 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Claudin 8 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

Claudin 8 Antibody (40-0700Z) in ICC/IF

Immunofluorescent co-localization (yellow) of Claudin-8 (red) and ZO-1 (green) with Rb anti-Claudin-8 (Product # 40-0700Z). Nuclei are labeled with DAPI (blue). Image courtesy of Jennifer Holmes and Dr. James Anderson, University of North Carolina at Chapel Hill, NC.

Claudin 8 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Claudin 8 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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Product Details

40-0700Z

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of mouse and rat Claudin-8.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533445

Target Information

Claudin-8 belongs to the claudin family of integral membrane proteins. Claudins are responsible for the formation of tight junction strands, and are connected with the actin cytoskeleton mediated by ZO-1. Claudin-8 is a tight junction protein expressed in several distal nephron segments, suggesting that it may have a role as a paracellular cation barrier protein. Claudin-8 is expressed in the inner ear, and the umbrella cell tight junctions in the bladder epithelium.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Claudin-2 promotes colorectal cancer liver metastasis and is a biomarker of the replacement type growth pattern.

Communications biology

Tabariès S,Annis MG,Lazaris A,Petrillo SK,Huxham J,Abdellatif A,Palmieri V,Chabot J,Johnson RM,Van Laere S,Verhoef C,Hachem Y,Yumeen S,Meti N,Omeroglu A,Altinel G,Gao ZH,Yu ASL,Grünhagen DJ,Vermeulen P,Metrakos P,Siegel PM

40-0700Z was used in Immunohistochemistry to provide evidence that Claudin-2 status in patient-derived extracellular vesicles may serve as a relevant prognostic biomarker to predict whether colorectal cancer patients have developed replacement type liver metastases.

Wed Jun 02 00:00:00 EDT 2021

Circulating Ouabain Modulates Expression of Claudins in Rat Intestine and Cerebral Blood Vessels.

International journal of molecular sciences

Markov AG,Fedorova AA,Kravtsova VV,Bikmurzina AE,Okorokova LS,Matchkov VV,Cornelius V,Amasheh S,Krivoi II

40-0700Z was used in Western Blotting to confirm that circulating ouabain can functionally and tissue-specifically affect barrier properties of epithelial and endothelial tissues via Na,K-ATPase-mediated modulation of claudins expression.

Fri Jul 17 00:00:00 EDT 2020

Circulating Ouabain Modulates Expression of Claudins in Rat Intestine and Cerebral Blood Vessels.

International journal of molecular sciences

Markov AG,Fedorova AA,Kravtsova VV,Bikmurzina AE,Okorokova LS,Matchkov VV,Cornelius V,Amasheh S,Krivoi II

Fri Jul 17 00:00:00 EDT 2020

Campylobacter jejuni Serine Protease HtrA Cleaves the Tight Junction Component Claudin-8.

Frontiers in cellular and infection microbiology

Sharafutdinov I,Esmaeili DS,Harrer A,Tegtmeyer N,Sticht H,Backert S

40-0700Z was used in Immunocytochemistry-immunoflourescence to study the interaction of HtrA with another human tight junction protein, claudin-8.

Thu Jun 17 00:00:00 EDT 2021

Campylobacter jejuni Serine Protease HtrA Cleaves the Tight Junction Component Claudin-8.

Frontiers in cellular and infection microbiology

Sharafutdinov I,Esmaeili DS,Harrer A,Tegtmeyer N,Sticht H,Backert S

40-0700Z was used in Immunocytochemistry-immunoflourescence to study the interaction of HtrA with another human tight junction protein, claudin-8.

Thu Jun 17 00:00:00 EDT 2021

Role of Claudins in Renal Branching Morphogenesis.

Physiological reports

El Andalousi J,Khairallah H,Zhuang Y,Ryan AK,Gupta IR

40-0700Z was used in Immunocytochemistry-Immunofluorescence to demonstrate that claudins are expressed in the ureteric bud lineage during the period of most rapid branching morphogenesis in vivo.

Tue Sep 01 00:00:00 EDT 2020

Role of Claudins in Renal Branching Morphogenesis.

Physiological reports

El Andalousi J,Khairallah H,Zhuang Y,Ryan AK,Gupta IR

40-0700Z was used in Immunocytochemistry-Immunofluorescence to demonstrate that claudins are expressed in the ureteric bud lineage during the period of most rapid branching morphogenesis in vivo.

Tue Sep 01 00:00:00 EDT 2020

Impact of Sensitization and Inflammation on the Interaction of Mast Cells With the Intestinal Epithelium in Rats.

Frontiers in physiology

Becker J,Ott D,Diener M

40-0700Z was used in Immunohistochemistry-immunofluorescence to study the impact of sensitisation and inflammation on the interaction of mast cells with the intestinal epithelium in rats.

Wed Sep 30 00:00:00 EDT 2020

Alterations of intercellular junctions in peritoneal mesothelial cells from patients undergoing dialysis: effect of retinoic Acid.

Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis

Retana C,Sanchez E,Perez-Lopez A,Cruz A,Lagunas J,Cruz C,Vital S,Reyes JL

40-0700Z was used in immunocytochemistry and western blot to determine the quantity, distribution and function of tight junction proteins in patients undergoing dialysis treatments

Tue Apr 05 00:00:00 EDT 2016

Alterations of intercellular junctions in peritoneal mesothelial cells from patients undergoing dialysis: effect of retinoic Acid.

Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis

Retana C,Sanchez E,Perez-Lopez A,Cruz A,Lagunas J,Cruz C,Vital S,Reyes JL

40-0700Z was used in immunocytochemistry and western blot to determine the quantity, distribution and function of tight junction proteins in patients undergoing dialysis treatments

Tue Apr 05 00:00:00 EDT 2016

Different effects of ZO-1, ZO-2 and ZO-3 silencing on kidney collecting duct principal cell proliferation and adhesion.

Cell cycle (Georgetown, Tex.)

Qiao X,Roth I,Féraille E,Hasler U

40-0700Z was used in immunocytochemistry to study the contribution of ZO-1, -2, and -3 on collecting duct cell proliferation.

Mon Aug 31 00:00:00 EDT 2015

Disrupted tight junctions in the small intestine of cystic fibrosis mice.

Cell and tissue research

De Lisle RC

40-0700Z was used in immunohistochemistry and western blot to characterize tight junctions in the digestive tract of cystic fibrosis mice

Wed Jan 01 00:00:00 EST 2014

Differential expression of claudin tight junction proteins in the human cortical nephron.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Kirk A,Campbell S,Bass P,Mason J,Collins J

Thu Jul 01 00:00:00 EDT 2010

Inward rectifier channel, ROMK, is localized to the apical tips of glial-like cells in mouse taste buds.

The Journal of comparative neurology

Dvoryanchikov G,Sinclair MS,Perea-Martinez I,Wang T,Chaudhari N

40-0700Z was used in immunohistochemistry to localize inward rectifier channel ROMK in the apical tips of glial-like cells in rodent taste buds

Sun Nov 01 00:00:00 EDT 2009

Loss of hepatocyte-nuclear-factor-4alpha affects colonic ion transport and causes chronic inflammation resembling inflammatory bowel disease in mice.

PloS one

Darsigny M,Babeu JP,Dupuis AA,Furth EE,Seidman EG,Lévy E,Verdu EF,Gendron FP,Boudreau F

Thu Oct 29 00:00:00 EDT 2009

Loss of hepatocyte-nuclear-factor-4alpha affects colonic ion transport and causes chronic inflammation resembling inflammatory bowel disease in mice.

PloS one

Darsigny M,Babeu JP,Dupuis AA,Furth EE,Seidman EG,Lévy E,Verdu EF,Gendron FP,Boudreau F

Thu Oct 29 00:00:00 EDT 2009

Claudin-based permeability barriers in taste buds.

The Journal of comparative neurology

Michlig S,Damak S,Le Coutre J

40-0700Z was used in immunohistochemistry to study the role of claudin in permeability barriers in taste buds

Wed Jun 20 00:00:00 EDT 2007

Claudin-based permeability barriers in taste buds.

The Journal of comparative neurology

Michlig S,Damak S,Le Coutre J

40-0700Z was used in immunohistochemistry to study the role of claudin in permeability barriers in taste buds

Wed Jun 20 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: Claudin-8

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Gene Aliases: AI648025; Cldn8

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UniProt ID: (Mouse) Q9Z260

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Entrez Gene ID: (Dog) 478401, (Rat) 304124, (Mouse) 54420

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Function(s)

structural molecule activity protein binding tight junction

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Claudin 8 Polyclonal Antibody

Claudin 8 Antibody (40-0700Z) in ICC/IF

Product Details

40-0700Z

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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