Invitrogen

Collagen I Polyclonal Antibody

View all (27) Collagen I antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA1-85312

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50
-

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:50
-

ELISA (ELISA)

1:500-1:3,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Collagen type I from human placenta.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Affinity chromatography

Storage buffer

0.01M sodium borate with 1% dextran, 1% mannitol, 0.1M NaCl

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_930051

Product Specific Information

This product requires antigen retrieval using 1 mg/mL pepsin in 0.5M acetic acid for 2 hours at 37ºC and multiple buffer washes prior to staining on paraffin sections.

Reconstitute with 0.5 mL of distilled water. Prior to reconstitution, store at 4ºC. After reconstitution, store undiluted at -20ºC, avoiding freeze/thaw cycles. For long term storage the addition of 1% BSA and 0.09% sodium azide is recommended.

Rabbit anti Human collagen I antibody recognizes human Collagen I, reacting with both native and heat denatured proteins, and has been cross-absorbed against Collagen types III, IV and V.

Target Information

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type I, alpha 1; collagen, type I, alpha 2; pro-alpha-1 collagen type 1; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain

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Gene Aliases: COL1A1; COL1A2; EDSC; OI1; OI2; OI3; OI4

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UniProt ID: (Human) P02452, (Human) P08123

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Entrez Gene ID: (Human) 1277, (Human) 1278

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Function(s)
extracellular matrix structural constituent protein binding identical protein binding metal ion binding platelet-derived growth factor binding protein binding, bridging SMAD binding extracellular matrix structural protein
Process(es)
skeletal system development blood vessel development osteoblast differentiation intramembranous ossification endochondral ossification blood coagulation visual perception sensory perception of sound positive regulation of epithelial to mesenchymal transition negative regulation of cell-substrate adhesion protein transport platelet activation extracellular matrix organization collagen fibril organization positive regulation of cell migration collagen catabolic process response to corticosteroid response to estradiol collagen biosynthetic process protein localization to nucleus tooth mineralization response to drug response to hydrogen peroxide response to peptide hormone skin morphogenesis cellular response to fibroblast growth factor stimulus tooth eruption positive regulation of transcription, DNA-templated embryonic skeletal system development regulation of immune response leukocyte migration response to cAMP response to hyperoxia face morphogenesis bone trabecula formation cartilage development involved in endochondral bone morphogenesis protein heterotrimerization cellular response to amino acid stimulus cellular response to mechanical stimulus cellular response to retinoic acid cellular response to vitamin E cellular response to tumor necrosis factor cellular response to epidermal growth factor stimulus cellular response to transforming growth factor beta stimulus positive regulation of canonical Wnt signaling pathway cellular response to fluoride transforming growth factor beta receptor signaling pathway Rho protein signal transduction regulation of blood pressure odontogenesis
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