Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
CSI 008-01-02 is highly specific for type I collagen. It has been shown to have no cross-reactivity with type II, III, V and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connnective tissue proteins (laminin, fibronectin, elastin). CSI 008-01-02 reacts with human, calf, sheep, pig, dog and goat type I collagens by ELISA and immunoblotting. No reactivity with mouse, rat, guinea pig, cat, rabbit, squirrel, horse, kangaroo or chicken type I collagen by ELISA and immunoblotting. CSI 008-01-02 has a Ka of approximately 2.5 x 10^6 for human type I collagen. CSI 008-01-02 has been successfully used in Western blotting, ELISA, and immunohistochemistry (frozen) procedures. In immunoblotting CSI 008-01 detects human type I collagen only in its native triple helix form. CSI 008-01 has been used successfully for immunohistology on frozen unfixed sections of human, and bovine skin, and for identification of collagen synthesis in cell culture. If fixation of tissue is required, acetone or ethanol is recommended. The specificity of the CSI 008-01-02 epitope is located near the C-terminal of the molecule. The CSI 008-01-02 immunogen is pepsin-solublized, salt-fractioned type I collagen from human placenta.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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