Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
MA1-83073
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
NFI/20
Immunogen
Human Collagen type I.
Conjugate
Form
Lyophilized
Purification
Ion-exchange chromatography, Ammonium sulfate precipitation
Storage buffer
PBS/0.01M sodium borate with 1% mannitol, 1% dextran
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_930061
Product Specific Information
MA1-83073 detects Collagen I in human sample types, and does not cross-react with collagen types II, III, or IV.
Reconstitute with 1 mL of distilled water. Prior to reconstitution, store at 4ºC. After reconstitution, store undiluted at -20ºC, avoiding freeze/thaw cycles.
Mouse anti Human Collagen I, clone NFI/20 recognizes human Collagen I composed of trimers consisting of 2 Collagen alpha-2(I) chains and a single Collagen alpha-1(I) chain forming collagen fibrils in ligaments, tendon, skin and bone where they become mineralized by calcium hydroxyapatite.
Target Information
Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type I, alpha 1; collagen, type I, alpha 2; pro-alpha-1 collagen type 1; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain
Gene Aliases: COL1A1; COL1A2; EDSC; OI1; OI2; OI3; OI4
UniProt ID: (Human) P02452, (Human) P08123
Entrez Gene ID: (Human) 1277, (Human) 1278
skeletal system development
blood vessel development
osteoblast differentiation
intramembranous ossification
endochondral ossification
blood coagulation
visual perception
sensory perception of sound
positive regulation of epithelial to mesenchymal transition
negative regulation of cell-substrate adhesion
protein transport
platelet activation
extracellular matrix organization
collagen fibril organization
positive regulation of cell migration
collagen catabolic process
response to corticosteroid
response to estradiol
collagen biosynthetic process
protein localization to nucleus
tooth mineralization
response to drug
response to hydrogen peroxide
response to peptide hormone
skin morphogenesis
cellular response to fibroblast growth factor stimulus
tooth eruption
positive regulation of transcription, DNA-templated
embryonic skeletal system development
regulation of immune response
leukocyte migration
response to cAMP
response to hyperoxia
face morphogenesis
bone trabecula formation
cartilage development involved in endochondral bone morphogenesis
protein heterotrimerization
cellular response to amino acid stimulus
cellular response to mechanical stimulus
cellular response to retinoic acid
cellular response to vitamin E
cellular response to tumor necrosis factor
cellular response to epidermal growth factor stimulus
cellular response to transforming growth factor beta stimulus
positive regulation of canonical Wnt signaling pathway
cellular response to fluoride
transforming growth factor beta receptor signaling pathway
Rho protein signal transduction
regulation of blood pressure
odontogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support