Invitrogen

Collagen I Recombinant Rabbit Monoclonal Antibody (ST58-04)

View all (27) Collagen I antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-32178

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000
-

Immunocytochemistry (ICC/IF)

1:2,000
-
Product Specifications

Species Reactivity

Bovine, Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ST58-04

Immunogen

Synthetic peptide within Human COL1A1 aa 1191-1238
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809466

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen 1 alpha 2 chain; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type I, alpha 1; collagen, type I, alpha 2; pro-alpha-1 collagen type 1; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain

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Gene Aliases: COL1A1; COL1A2; EDSC; OI1; OI2; OI3; OI4

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UniProt ID: (Human) P02452, (Bovine) P02453, (Human) P08123, (Bovine) P02465

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Entrez Gene ID: (Human) 1277, (Bovine) 282187, (Human) 1278, (Bovine) 282188

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Function(s)
extracellular matrix structural constituent protein binding identical protein binding metal ion binding platelet-derived growth factor binding protease binding protein binding, bridging SMAD binding extracellular matrix structural protein
Process(es)
skeletal system development blood vessel development osteoblast differentiation intramembranous ossification endochondral ossification blood coagulation visual perception sensory perception of sound positive regulation of epithelial to mesenchymal transition negative regulation of cell-substrate adhesion protein transport platelet activation extracellular matrix organization collagen fibril organization positive regulation of cell migration collagen catabolic process response to corticosteroid response to estradiol collagen biosynthetic process protein localization to nucleus tooth mineralization response to drug response to hydrogen peroxide response to peptide hormone skin morphogenesis cellular response to fibroblast growth factor stimulus tooth eruption positive regulation of transcription, DNA-templated embryonic skeletal system development regulation of immune response leukocyte migration response to cAMP response to hyperoxia face morphogenesis bone trabecula formation cartilage development involved in endochondral bone morphogenesis protein heterotrimerization cellular response to amino acid stimulus cellular response to mechanical stimulus cellular response to retinoic acid cellular response to vitamin E cellular response to tumor necrosis factor cellular response to epidermal growth factor stimulus cellular response to transforming growth factor beta stimulus positive regulation of canonical Wnt signaling pathway cellular response to fluoride transforming growth factor beta receptor signaling pathway Rho protein signal transduction regulation of blood pressure odontogenesis bone mineralization collagen metabolic process extracellular matrix assembly
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