Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Collagen II Polyclonal Antibody
FIGURE: 1 / 2
Collagen II Antibody (PA1-26206) in IHC (P)
Immunohistochemistry (Paraffin) analysis of Collagen II was performed in FFPE human bronchiolar cartilage tissue using Collagen II Polyclonal Antibody (Product # PA1-26206) at a dilution of 1:400 for 45 min at RT. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.
Product Details
PA1-26206
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Collagen type II from adult human knee cartilage and bovine nasal cartilage.
Conjugate
Form
Liquid
Concentration
1.03 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.020M potassium phosphate, pH 8.0, with 0.150M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_779883
Product Specific Information
Recommended positive controls: chondrocyte tissue.
PA1-26206 is expected to cross react with most mammals due to sequence homology. This antibody shows negligable cross reactivity with the Collagens 1, 3, 4, 5, and 6.
Target Information
COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Alpha-1 type II collagen; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CGA; chondrocalcin; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type II (Col2A1) gene, enhancer region; collagen, type II, alpha 1; MGC131516; pro-alpha 1(II); Procollagen II alpha 1; procollagen, type II, alpha 1; unnamed protein product
Gene Aliases: ANFH; AOM; CB11; CG2A1A; COL11A3; COL2A1; COLLII; SEDC; STL1
UniProt ID: (Human) P02458, (Bovine) P02459, (Rat) P05539
Entrez Gene ID: (Human) 1280, (Bovine) 407142, (Rat) 25412
extracellular matrix structural constituent conferring tensile strength
identical protein binding
metal ion binding
platelet-derived growth factor binding
protein binding
MHC class II protein binding
proteoglycan binding
extracellular matrix structural constituent
extracellular matrix structural protein
skeletal system development
cartilage condensation
tissue homeostasis
endochondral ossification
chondrocyte differentiation
heart morphogenesis
proteoglycan metabolic process
central nervous system development
visual perception
sensory perception of sound
regulation of gene expression
extracellular matrix organization
collagen fibril organization
collagen catabolic process
notochord development
inner ear morphogenesis
regulation of immune response
cartilage development
palate development
limb bud formation
embryonic skeletal joint morphogenesis
cartilage development involved in endochondral bone morphogenesis
otic vesicle development
cellular response to BMP stimulus
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
anterior head development
growth plate cartilage development
response to mechanical stimulus
response to X-ray
limb morphogenesis
skeletal system morphogenesis
inner ear development
bone development
cellular response to mechanical stimulus
cellular response to retinoic acid
cellular response to vitamin E
cellular response to nicotine
cellular response to tumor necrosis factor
cellular response to parathyroid hormone stimulus
cellular response to peptide hormone stimulus
response to fibroblast growth factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support