Invitrogen

Collagen II Polyclonal Antibody

Collagen II Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Collagen II Antibody (PA5-99159) in IHC (P)

Immunohistochemistry analysis of Collagen II in human lung cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Collagen II polyclonal antibody (Product # PA5-99159) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Collagen II Antibody in Western Blot (WB)

Collagen II Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

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Product Details

PA5-99159

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human COL2A1(Accession P02458), corresponding to amino acid residues K113-P163.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2818092

Product Specific Information

Antibody detects endogenous levels of total Collagen II.

Target Information

COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Caffeic acid phenethyl ester attenuates osteoarthritis progression by activating NRF2/HO‑1 and inhibiting the NF‑κB signaling pathway.

International journal of molecular medicine

Sun W,Xie W,Huang D,Cui Y,Yue J,He Q,Jiang L,Xiong J,Sun W,Yi Q

PA5-99159 was used in Western Blot, Immunohistochemistry to investigate the antiinflammatory effects of CAPE on IL‚1β‚stimulated chondrocytes in vitro and surgically induced rat models of OA in vivo.

Tue Nov 01 00:00:00 EDT 2022

Caffeic acid phenethyl ester attenuates osteoarthritis progression by activating NRF2/HO‑1 and inhibiting the NF‑κB signaling pathway.

International journal of molecular medicine

Sun W,Xie W,Huang D,Cui Y,Yue J,He Q,Jiang L,Xiong J,Sun W,Yi Q

Tue Nov 01 00:00:00 EDT 2022

Pharmacological Disruption of Phosphorylated Eukaryotic Initiation Factor-2α/Activating Transcription Factor 4/Indian Hedgehog Protects Intervertebral Disc Degeneration via Reducing the Reactive Oxygen Species and Apoptosis of Nucleus Pulposus Cells.

Frontiers in cell and developmental biology

Bao J,Qian Z,Liu L,Hong X,Che H,Wu X

PA5-99159 was used in Immunohistochemistry to investigate the impact of phosphorylation of eukaryotic initiation factor-2α (eIF2α)/activating transcription factor 4 (ATF4)/Indian hedgehog (Ihh) signalling in intervertebral disc degeneration.

Fri Jun 25 00:00:00 EDT 2021

Frontiers in cell and developmental biology

Bao J,Qian Z,Liu L,Hong X,Che H,Wu X

Fri Jun 25 00:00:00 EDT 2021

Frontiers in cell and developmental biology

Bao J,Qian Z,Liu L,Hong X,Che H,Wu X

Fri Jun 25 00:00:00 EDT 2021

Nanoscale Thermosensitive Hydrogel Scaffolds Promote the Chondrogenic Differentiation of Dental Pulp Stem and Progenitor Cells: A Minimally Invasive Approach for Cartilage Regeneration.

International journal of nanomedicine

Talaat W,Aryal Ac S,Al Kawas S,Samsudin ABR,Kandile NG,Harding DRK,Ghoneim MM,Zeiada W,Jagal J,Aboelnaga A,Haider M

PA5-99159 was used in Immunohistochemistry to prepare nanocellulose-based thermosensitive injectable hydrogel scaffolds and assess their potential as 3D scaffolds allowing the chondrogenic differentiation of embedded human dental pulp stem and progenitor cells (hDPSCs).

Fri Nov 20 00:00:00 EST 2020

Nanoscale Thermosensitive Hydrogel Scaffolds Promote the Chondrogenic Differentiation of Dental Pulp Stem and Progenitor Cells: A Minimally Invasive Approach for Cartilage Regeneration.

International journal of nanomedicine

Talaat W,Aryal Ac S,Al Kawas S,Samsudin ABR,Kandile NG,Harding DRK,Ghoneim MM,Zeiada W,Jagal J,Aboelnaga A,Haider M

Fri Nov 20 00:00:00 EST 2020

Nanoscale Thermosensitive Hydrogel Scaffolds Promote the Chondrogenic Differentiation of Dental Pulp Stem and Progenitor Cells: A Minimally Invasive Approach for Cartilage Regeneration.

International journal of nanomedicine

Talaat W,Aryal Ac S,Al Kawas S,Samsudin ABR,Kandile NG,Harding DRK,Ghoneim MM,Zeiada W,Jagal J,Aboelnaga A,Haider M

Fri Nov 20 00:00:00 EST 2020

Bioinformatics

Protein Aliases: Alpha-1 type II collagen; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CGA; chondrocalcin; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type II (Col2A1) gene, enhancer region; collagen, type II, alpha 1; disproportionate micromelia; MGC131516; Procollagen II alpha 1; procollagen, type II, alpha 1

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Gene Aliases: ANFH; AOM; CG2A1A; COL11A3; Col2; Col2a; Col2a-1; COL2A1; COLLII; Del1; Dmm; Lpk; M100856; Rgsc856; SEDC; STL1

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UniProt ID: (Human) P02458, (Rat) P05539, (Mouse) P28481

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Entrez Gene ID: (Human) 1280, (Rat) 25412, (Mouse) 12824

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Function(s)

extracellular matrix structural constituent conferring tensile strength identical protein binding metal ion binding platelet-derived growth factor binding extracellular matrix structural constituent extracellular matrix structural protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Collagen II Polyclonal Antibody

Collagen II Antibody (PA5-99159) in IHC (P)

Product Details

PA5-99159

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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