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Invitrogen
Collagen III Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
Collagen III Antibody (PA1-28870) in IHC (P)
Immunohistochemistry analysis of Collagen III was performed in human skin and testis tissue using Collagen III Polyclonal Antibody (Product # PA1-28870) at a dilution of 1:400 for 45 minutes a room temperature. The antibody showed strong staining in FFPE sections of human skin (left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue b... View More
Product Details
PA1-28870
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Collagen Type III from human and bovine placenta
Conjugate
Form
Liquid
Concentration
1.16 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_1956965
Product Specific Information
PA1-28870 has been successfully used in ELISA, immunohistochemistry (frozen tissue), immunoprecipitation, and Western blot applications. NOTE: The preparation of a specific antibody to Collagen III's uninterrupted "Glycine-X-Y" triplet repeat requires a non-denatured epitope format. Consequently, it is recommended to use this antibody in a Western blot using non-denaturing or "native" conditions.
Store as concentrated solution. Centrifuge briefly prior to opening vial.
Target Information
Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in th gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha1 (III) collagen; COL 3; COL3; COL3 alpha 1; Collagen 3; Collagen alpha-1(III) chain; collagen, fetal; collagen, type III, alpha 1; collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant); collagen, type III, alpha 1 chain, fibril forming; Collagen3; Ehlers-Danlos syndrome type IV, autosomal dominant; FLJ34534; MGC93704
Gene Aliases: COL3A1; EDS4A
UniProt ID: (Human) P02461
Entrez Gene ID: (Bovine) 510833, (Human) 1281
cerebral cortex development
positive regulation of Rho protein signal transduction
negative regulation of neuron migration
skeletal system development
cell-matrix adhesion
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
heart development
aging
response to radiation
response to mechanical stimulus
peptide cross-linking
platelet activation
extracellular matrix organization
collagen fibril organization
collagen catabolic process
response to cytokine
wound healing
extracellular fibril organization
skin development
digestive tract development
regulation of immune response
negative regulation of immune response
aorta smooth muscle tissue morphogenesis
cellular response to amino acid stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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