Invitrogen

Collagen III Polyclonal Antibody

2 Published Figures

10 References

View all (40) Collagen III antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (2)

Collagen III Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 6

Collagen III Antibody (PA5-27828) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human PAPILLARY CA_STROMAL CELLS, using Collagen III (Product # PA5-27828) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Collagen III Antibody in Western Blot (WB)

Collagen III Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))

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Product Details

PA5-27828

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 1180 and 1444 of Human COL3A1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.46 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545304

Product Specific Information

Recommended positive controls: HeLa, COL3A1 shRNA-transfected HeLa, SK-N-SH, SK-N-AS, Neuro2A, NIH-3T3, BCL-1, Raw264.7, C2C12, Mouse ESC, PC-12, Rat2, rat liver.

Predicted reactivity: Mouse (92%), Rat (92%), Xenopus laevis (80%), Pig (94%), Chicken (83%), Rhesus Monkey (98%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in th gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Scutellarin Attenuates Doxorubicin-Induced Cardiotoxicity by Inhibiting Myocardial Fibrosis, Apoptosis and Autophagy in Rats.

Chemistry & biodiversity

Sun X,Zhou L,Han Y,Yang Q,Li X,Xin B,Chi M,Wang Y,Guo C

PA5-27828 was used in Western Blot to reveal that the cardioprotective effect of SCU on DOX-induced chronic cardiotoxicity may be attributed to reducing oxidative stress, myocardial fibrosis, apoptosis and autophagy.

Sun Jan 01 00:00:00 EST 2023

Downregulation of senescence-associated secretory phenotype by knockdown of secreted frizzled-related protein 4 contributes to the prevention of skin aging.

Aging

Takaya K,Asou T,Kishi K

PA5-27828 was used in Western Blotting to suggest a potential candidate for the development of new skin rejuvenation therapies that suppress SASP.

Wed Sep 07 00:00:00 EDT 2022

Effects and Mechanism of Particulate Matter on Tendon Healing Based on Integrated Analysis of DNA Methylation and RNA Sequencing Data in a Rat Model.

International journal of molecular sciences

Lee SY,Lee MH,Jo SK,Yoo IH,Sarankhuu BE,Kim HJ,Kang YE,Lee SE,Kim TY,Park MH,Lee CS,Han SY,Moon JH,Jung JY,Hong GL,Yoo NJ,Yoon ES,Choi JK,Won HR,Son JW,Song JH

PA5-27828 was used in Western Blotting to investigate the relationship between particulate matter exposure and tendon healing.

Mon Jul 25 00:00:00 EDT 2022

Impaired non-canonical transforming growth factor-β signalling prevents profibrotic phenotypes in cultured peptidylarginine deiminase 4-deficient murine cardiac fibroblasts.

Journal of cellular and molecular medicine

Akboua H,Eghbalzadeh K,Keser U,Wahlers T,Paunel-Görgülü A

PA5-27828 was used in Western Blot to investigate the impact of peptidyl arginine deiminase 4 on cardiac fibroblast function and myofibroblast trans differentiation in vitro.

Fri Oct 01 00:00:00 EDT 2021

Impaired non-canonical transforming growth factor-β signalling prevents profibrotic phenotypes in cultured peptidylarginine deiminase 4-deficient murine cardiac fibroblasts.

Journal of cellular and molecular medicine

Akboua H,Eghbalzadeh K,Keser U,Wahlers T,Paunel-Görgülü A

PA5-27828 was used in Western Blot to investigate the impact of peptidyl arginine deiminase 4 on cardiac fibroblast function and myofibroblast trans differentiation in vitro.

Fri Oct 01 00:00:00 EDT 2021

Necrostatin-1, RIP1/RIP3 inhibitor, relieves transforming growth factor β-induced wound-healing process in formation of hypertrophic scars.

Journal of cosmetic dermatology

Lin PT,Xue XD,Zhao ZD,Lu JY,Xie PL

PA5-27828 was used in Immunohistochemistry (Paraffin), Western Blot to investigate Necrostatin-1 (Nec-1), a RIP1/RIP3 inhibitor, in the formation of hypertrophic scar.

Sun Aug 01 00:00:00 EDT 2021

Cathepsin B deficiency ameliorates liver lipid deposition, inflammatory cell infiltration, and fibrosis after diet-induced nonalcoholic steatohepatitis.

Translational research : the journal of laboratory and clinical medicine

Fang W,Deng Z,Benadjaoud F,Yang C,Shi GP

PA5-27828 was used in Western Blot to show that CatB deficiency improves liver function in NASH mice by suppressing de novo lipogenesis and liver inflammation and fibrosis.

Sat Aug 01 00:00:00 EDT 2020

Chronic High Glucose Concentration Induces Inflammatory and Remodeling Changes in Valvular Endothelial Cells and Valvular Interstitial Cells in a Gelatin Methacrylate 3D Model of the Human Aortic Valve.

Polymers

Ciortan L,Macarie RD,Cecoltan S,Vadana M,Tucureanu MM,Mihaila AC,Droc I,Butoi E,Manduteanu I

PA5-27828 was used in Immunohistochemistry (Frozen) to study the effect of high glucose on valvular cell phenotype and function in a 3D model.

Wed Nov 25 00:00:00 EST 2020

Polymers

Ciortan L,Macarie RD,Cecoltan S,Vadana M,Tucureanu MM,Mihaila AC,Droc I,Butoi E,Manduteanu I

PA5-27828 was used in Immunohistochemistry (Frozen) to study the effect of high glucose on valvular cell phenotype and function in a 3D model.

Wed Nov 25 00:00:00 EST 2020

Polymers

Ciortan L,Macarie RD,Cecoltan S,Vadana M,Tucureanu MM,Mihaila AC,Droc I,Butoi E,Manduteanu I

PA5-27828 was used in Immunohistochemistry (Frozen) to study the effect of high glucose on valvular cell phenotype and function in a 3D model.

Wed Nov 25 00:00:00 EST 2020

ACVR1^R206H FOP mutation alters mechanosensing and tissue stiffness during heterotopic ossification.

Molecular biology of the cell

Haupt J,Stanley A,McLeod CM,Cosgrove BD,Culbert AL,Wang L,Mourkioti F,Mauck RL,Shore EM

PA5-27828 was used in Immunohistochemistry to examine the fibroproliferative tissue preceding heterotopic bone and identified pathologic stiffening at this stage of repair.

Tue Jan 01 00:00:00 EST 2019

Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.

Scientific reports

Buken C,Sahana J,Corydon TJ,Melnik D,Bauer J,Wehland M,Krüger M,Balk S,Abuagela N,Infanger M,Grimm D

PA5-27828 was used in Flow cytometry/Cell sorting to show simulated microgravity causes changes in the cytoskeleton, extracellular matrix, focal adhesion and growth behaviour of normal human dermal fibroblasts and to identify the factors involved in the assembly of the fibroblasts.

Thu Aug 15 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: alpha1 (III) collagen; COL 3; COL3; COL3 alpha 1; Collagen 3; Collagen alpha-1(III) chain; collagen, fetal; collagen, type III, alpha 1; Collagen3; Ehlers-Danlos syndrome type IV, autosomal dominant; FLJ34534; MGC93704

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Gene Aliases: COL3A1; EDS4A

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UniProt ID: (Human) P02461

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Entrez Gene ID: (Human) 1281

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Function(s)

integrin binding extracellular matrix structural constituent protein binding SMAD binding metal ion binding platelet-derived growth factor binding extracellular matrix structural protein

Process(es)

skeletal system development cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway heart development aging response to radiation response to mechanical stimulus peptide cross-linking cerebral cortex development platelet activation extracellular matrix organization collagen fibril organization collagen catabolic process response to cytokine positive regulation of Rho protein signal transduction wound healing extracellular fibril organization skin development digestive tract development regulation of immune response negative regulation of immune response aorta smooth muscle tissue morphogenesis cellular response to amino acid stimulus negative regulation of neuron migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Collagen III Polyclonal Antibody

Collagen III Antibody (PA5-27828) in IHC (P)

Product Details

PA5-27828

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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