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Invitrogen
Collagen III (soluble) Monoclonal Antibody (1E7-D7/Col3)
Product Details
CSI 007-01-02
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Marsupial,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
1E7-D7/Col3
Immunogen
Acid-digested pepsin soluble human type III collagen.
Conjugate
Form
Liquid
Concentration
1.04 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1073771
Product Specific Information
CSI 007-01-02 is highly specific for type III collagen. It has been shown to have no cross-reactivity with type I and V collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). CSI 007-01-02 shows reactivity with human, dog, rat and kangaroo in type III collagens by ELISA and immunoblotting. The antibody does not show reactivity with calf and sheep type III collagen by ELISA and immunoblotting.
CSI 007-01-02 has been successfully used in Western blotting, ELISA, and immunohistochemistry (frozen) procedures. CSI 007-01 has been used successfully for immunohistology on frozen unfixed sections of human and kangaroo skin, and of new dog tissue associated with biomaterial implants. If fixation of tissue is required, acetone or ethanol is recommended.
The specificity of the CSI 007-01-02 epitope is located approximately 100 nm from the N-terminal of the molecule.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in th gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha1 (III) collagen; COL 3; COL3; COL3 alpha 1; Collagen 3; collagen alpha 1 type III; Collagen alpha-1(III) chain; collagen, fetal; collagen, type III, alpha 1; collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant); Collagen3; Ehlers-Danlos syndrome type IV, autosomal dominant; FLJ34534; MGC93704; procollagen, type III, alpha 1
Gene Aliases: COL3A1; EDS4A; PIIINP
UniProt ID: (Human) P02461, (Rat) P13941
Entrez Gene ID: (Dog) 478835, (Human) 1281, (Rat) 84032
skeletal system development
cell-matrix adhesion
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
heart development
aging
response to radiation
response to mechanical stimulus
peptide cross-linking
cerebral cortex development
platelet activation
extracellular matrix organization
collagen fibril organization
collagen catabolic process
response to cytokine
positive regulation of Rho protein signal transduction
wound healing
extracellular fibril organization
skin development
digestive tract development
regulation of immune response
negative regulation of immune response
aorta smooth muscle tissue morphogenesis
cellular response to amino acid stimulus
negative regulation of neuron migration
blood vessel development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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