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Collagen Type I Antibody (600-406-103) in WB
Western Blot of Rabbit anti-Collagen I antibody. Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 µg per lane. Protein staining shown below western blot depicts equal protein loading. Primary antibody: Anti-Collagen I antibody at 0.2-2 µg/10 ml for overnight at 4°C. Secondary antibody: horseradish peroxidase-conjugated rabbit secondary antibody at 1 µg/10 ml for overnight at 4°C. Block: TBS with 5% Non-fat milk. Predicted/Observed size: 138.9 kDa for Collag... View More
Product Details
600-406-103
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Collagen Type I from human and bovine placenta.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Target Information
Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha-1 type 1 collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen 1 alpha 2 chain; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen COL1A2; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; osteogenesis imperfecta; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagen
Gene Aliases: AA960264; AI325291; Col1a-1; Col1a-2; COL1A1; COL1A2; Cola-1; Cola-2; Cola1; Cola2; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4; oim
UniProt ID: (Human) P02452, (Bovine) P02453, (Human) P08123, (Bovine) P02465, (Rat) P02454, (Mouse) P11087, (Mouse) Q01149, (Rat) P02466
Entrez Gene ID: (Human) 1277, (Bovine) 282187, (Human) 1278, (Bovine) 282188, (Rat) 29393, (Mouse) 12842, (Mouse) 12843, (Rat) 84352
skeletal system development
blood vessel development
osteoblast differentiation
intramembranous ossification
endochondral ossification
blood coagulation
visual perception
sensory perception of sound
positive regulation of epithelial to mesenchymal transition
negative regulation of cell-substrate adhesion
protein transport
platelet activation
extracellular matrix organization
collagen fibril organization
positive regulation of cell migration
collagen catabolic process
response to corticosteroid
response to estradiol
collagen biosynthetic process
protein localization to nucleus
tooth mineralization
response to drug
response to hydrogen peroxide
response to peptide hormone
skin morphogenesis
cellular response to fibroblast growth factor stimulus
tooth eruption
positive regulation of transcription, DNA-templated
embryonic skeletal system development
regulation of immune response
leukocyte migration
response to cAMP
response to hyperoxia
face morphogenesis
bone trabecula formation
cartilage development involved in endochondral bone morphogenesis
protein heterotrimerization
cellular response to amino acid stimulus
cellular response to mechanical stimulus
cellular response to retinoic acid
cellular response to vitamin E
cellular response to tumor necrosis factor
cellular response to epidermal growth factor stimulus
cellular response to transforming growth factor beta stimulus
positive regulation of canonical Wnt signaling pathway
cellular response to fluoride
transforming growth factor beta receptor signaling pathway
Rho protein signal transduction
regulation of blood pressure
odontogenesis
bone mineralization
collagen metabolic process
extracellular matrix assembly
ossification
response to nutrient
response to mechanical stimulus
response to nutrient levels
wound healing
skin development
response to steroid hormone
skeletal system morphogenesis
response to fluoride
cellular response to organic substance
response to norepinephrine
cellular response to thyroid hormone stimulus
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