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Collagen Type II Antibody (600-403-104) in IHC (P)
Immunohistochemistry of Rabbit Anti-Collagen II Biotin Conjugated Antibody. Tissue: human bronchiolar cartilage (shown). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skele... View More
Product Details
600-403-104
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Collagen Type II antibody was produced by repeated immunizations with Collagen Type II from adult human knee cartilage and bovine nasal cartilage.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 10mg/mL BSA
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Target Information
COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-1 type II collagen; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CGA; chondrocalcin; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen, type II, alpha 1; MGC131516; pro-alpha 1(II); unnamed protein product
Gene Aliases: ANFH; AOM; CB11; COL11A3; COL2A1; SEDC; STL1
UniProt ID: (Human) P02458, (Bovine) P02459
Entrez Gene ID: (Human) 1280, (Bovine) 407142
skeletal system development
cartilage condensation
tissue homeostasis
endochondral ossification
chondrocyte differentiation
heart morphogenesis
proteoglycan metabolic process
central nervous system development
visual perception
sensory perception of sound
regulation of gene expression
extracellular matrix organization
collagen fibril organization
collagen catabolic process
notochord development
inner ear morphogenesis
regulation of immune response
cartilage development
palate development
limb bud formation
embryonic skeletal joint morphogenesis
cartilage development involved in endochondral bone morphogenesis
otic vesicle development
cellular response to BMP stimulus
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
anterior head development
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