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Invitrogen
Collagen X Recombinant Rabbit Monoclonal Antibody (ARC0659)
FIGURE: 1 / 1
Collagen X Antibody (MA5-35145) in WB
Western blot analysis of Collagen X in extracts of various cell lines. Samples were incubated with Collagen X Monoclonal antibody (Product # MA5-35145) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-35145
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC0659
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Collagen X/COL10A1 (Q03692)
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849050
Product Specific Information
Immunogen sequence: HSGEPGLPGP PGPPGPPGQA VMPEGFIKAG QRPSLSGTPL VSANQGVTGM PVSAFTVILS KAYPAIGTPI PFDKILYNRQ QHYDPRTGIF TCQIPGIYYF S
Target Information
Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Collagen alpha-1(X) chain; pro alpha 1; procollagen, type X, alpha 1
Gene Aliases: Col10; Col10a-1; Col10a1
UniProt ID: (Mouse) Q05306
Entrez Gene ID: (Mouse) 12813, (Rat) 25681
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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