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Complement C3a Antibody (MA1-40158) in WB
Western blot was performed using Anti-Complement C3a Monoclonal Antibody (2898) (Product # MA1-40158) and a 187 kDa band corresponding to Complement C3 was observed in HepG2 in comparison to HEK-293 which is reported to be negative. A band around 120 kDa was also observed in Hep G2 treated with LPS and PTI which can be the processed form of complement C3. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with protein transport inhibitor (1X for 4 hr) (Lane 2) and Hep G2 treated with LPS (10 ng/mL for 18 hr) followed by ... View More
Product Details
MA1-40158
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2898
Immunogen
C-terminus of C3.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein G
Storage buffer
PBS with 0.1% BSA
Contains
0.02% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1073817
Product Specific Information
MA1-40158 detects C3/C3a from human samples.
MA1-40158 has been successfully used in Western blot and ELISA applications.
The MA1-40158 immunogen is the C-terminus of C3.
Positive control: Human plasma; Negative control: C3-depleted serum
Target Information
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; C3r1; Complement C3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; prepro-C3
Gene Aliases: AHUS5; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p
UniProt ID: (Human) P01024
Entrez Gene ID: (Human) 718
positive regulation of type IIa hypersensitivity
positive regulation of protein phosphorylation
positive regulation of activation of membrane attack complex
tolerance induction
proteolysis
fatty acid metabolic process
inflammatory response
immune response
complement activation
complement activation, alternative pathway
complement activation, classical pathway
signal transduction
G-protein coupled receptor signaling pathway
blood coagulation
positive regulation of vascular endothelial growth factor production
positive regulation of glucose transport
regulation of triglyceride biosynthetic process
positive regulation of lipid storage
negative regulation of endopeptidase activity
regulation of complement activation
response to magnesium ion
response to estradiol
response to progesterone
response to estrogen
positive regulation of G-protein coupled receptor protein signaling pathway
positive regulation of angiogenesis
positive regulation of developmental growth
regulation of immune response
response to glucocorticoid
positive regulation of ERK1 and ERK2 cascade
positive regulation of apoptotic cell clearance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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