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Invitrogen
Complement C5a Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
Complement C5a Antibody (PA5-47260) in Neu
Neutralization of Complement C5a in dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line. Samples were incubated in Complement C5a polyclonal antibody (Product # PA5-47260). Recombinant Mouse Complement Component C5a inducesN‚acetyl- beta -D-glucosaminidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N‚Acetyl- beta -D-Glucosaminidase Release elicited by Recombinant Mouse Complement Component C5a (100 ng/mL) is neutralized (gr... View More
Product Details
PA5-47260
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse Complement Component C5a Asn679-Arg755
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609686
Product Specific Information
In direct ELISAs and Western blots (non-reducing conditions), approximately 50% cross-reactivity with recombinant human Complement Component C5a is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C4B1; C4B12; C4B2; C4B3; C4B5; C4F; CH; CO4; Complement C5; CPAMD3; DADB-112B14.11; FLJ17816; FLJ17822; FLJ60561; Hemolytic complement; MGC142298; MGC164979
Gene Aliases: C5; C5a; Hc; He
UniProt ID: (Mouse) P06684
Entrez Gene ID: (Mouse) 15139
in utero embryonic development
immune system process
inflammatory response
complement activation, alternative pathway
complement activation, classical pathway
positive regulation of vascular endothelial growth factor production
negative regulation of macrophage chemotaxis
cytolysis
innate immune response
positive regulation of angiogenesis
positive regulation of chemokine secretion
mitophagy in response to mitochondrial depolarization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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